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  4. Recombinant Mouse Core histone macro-H2A.1 (H2afy)

Recombinant Mouse Core histone macro-H2A.1 (H2afy)

  • 货号：
    CSB-YP879480MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP879480MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP879480MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP879480MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP879480MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Macroh2a1; H2afy; Core histone macro-H2A.1; Histone macroH2A1; mH2A1; H2A.y; H2A/y
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-372
  • 氨基酸序列
    MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVAKKTG GKKGARKSKK KQGEVSKAAS ADSTTEGTPT DGFTVLSTKS LFLGQKLNLI HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK KNGPLEVAGA AISAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL ADDRKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY FMLFDSESIG IYVQEMAKLD AN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.; Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.; Represses SOD3 gene expression.
  • 基因功能参考文献：
    1. These data indicate that macroH2A1.1-containing chromatin regulates mitochondrial respiration by limiting nuclear NAD(+) consumption and establishing a buffer of NAD(+) precursors in differentiated cells. PMID: 28991266
    2. Both histone H3 lysine 27 acetylation and recruitment of the transcription factor Pbx1 at prospective enhancers are regulated by mH2A1.2. PMID: 26832413
    3. The Skp2-mH2A1-CDK8 axis has a critical role in breast cancer development via dysregulation of the G2/M transition, polyploidy, cell growth dysregulation, and loss of tumor suppression. PMID: 25818643
    4. tumor suppressive histone variant macroH2A1 is a critical component of the positive feedback loop that maintains the cell senescence-associated secretory phenotype, including activation of ATM PMID: 26300260
    5. The macroH2A, a critical component of chromatin, suppresses the development of melanoma through regulating cyclin D1, cyclin D3 and CDK6 genes. PMID: 25119498
    6. Data show that MacroH2A.1 and macroH2A.2 regulate gene expression with them acting synergistically on the expression of some genes and apparently having opposing effects on others. PMID: 25312643
    7. The results demonstrate that macroH2A1 is a new factor involved in the regulation of rDNA transcription. PMID: 24071584
    8. Both macroH2A1 isoforms may play a role in hepatocellular carcinoma pathogenesis. PMID: 23372727
    9. macroH2A1 and macroH2A2, together with histone 3 lysine 27 methylated 3, co-occupy pluripotency genes in wild-type (wt) fibroblasts. PMID: 23463008
    10. macroH2A1 is a critical chromatin component that regulates the delicate balance between self-renewal and differentiation of embryonic and adult stem cells PMID: 22331466
    11. H2AFY is specifically overexpressed in two models of Huntington disease PMID: 21969577
    12. MacroH2A1 does not localize to the centrosome. PMID: 21364955
    13. Decreased stability of the inactive X chromosome is revealed in mouse epiblast stem cells, highlighting the importance of combinatorial epigenetic repression involving macroH2A in restricting transcriptional reprogramming by oocytes. PMID: 21552206
    14. Knockdown of mH2A isoforms in melanoma cells of low malignancy results in significantly increased proliferation and migration in vitro and growth and metastasis in vivo PMID: 21179167
    15. macroH2A1 functions primarily as a repressor in adult liver. This repressor activity is further supported by the substantial and relatively uniform macroH2A1 enrichment along the inactive X chromosome, which averages 4-fold. PMID: 20937776
    16. C-terminal amino acids of H2A.X function specifically to target this variant histone into chromatin in embryos after fertilization and that the absence of H2A.Z and macroH2A from the chromatin is required for normal development. PMID: 20943707
    17. Rapid elimination of MacroH2A from somatic cell heterochromatin after nuclear transfer is reported. PMID: 20132012
    18. distributed during the maintainance phase of x inactivation throughout cell cycle PMID: 12082075
    19. A maternal store of macroH2A is removed from the maternal genome prior to synkaryogamy. PMID: 15680357
    20. distribution of macroH2A1 histone variants in liver chromatin PMID: 16738309
    21. The function of macroH2A1 histones is not restricted to gene silencing but also involves fine tuning the expression of specific genes. PMID: 17242180
    22. macroH2A1 histones have a significant role in silencing endogenous MLVs in vivo. Specific internal MLV sequences are targeted by a macroH2A1-dependent silencing mechanism. PMID: 18195046

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 组织特异性：
    Widely expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, expressed in proximal and distal convoluted
  • 数据库链接：

    KEGG: mmu:26914

    STRING: 10090.ENSMUSP00000016081

    UniGene: Mm.283802