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  4. Recombinant Mouse Class E basic helix-loop-helix protein 40 (Bhlhe40)

Recombinant Mouse Class E basic helix-loop-helix protein 40 (Bhlhe40)

  • 货号：
    CSB-YP002691MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002691MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002691MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002691MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002691MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Bhlhe40
  • Uniprot No.：
  • 别名：
    Bhlhe40; Bhlhb2; Clast5; Dec1; Stra13Class E basic helix-loop-helix protein 40; bHLHe40; Class B basic helix-loop-helix protein 2; bHLHb2; Differentially expressed in chondrocytes protein 1; DEC1; E47 interaction protein 1; EIP1; Stimulated by retinoic acid gene 13 protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-411
  • 氨基酸序列
    MERIPSAQPP PTCLPKAPGL EHGDLSGMDF AHMYQVYKSR RGIKRSEDSK ETYKLPHRLI EKKRRDRINE CIAQLKDLLP EHLKLTTLGH LEKAVVLELT LKHVKALTNL IDQQQQKIIA LQSGLQAGDL SGRNLEAGQE MFCSGFQTCA REVLQYLAKH ENTRDLKSSQ LVTHLHRVVS ELLQGGASRK PLDSAPKAVD LKEKPSFLAK GSEGPGKNCV PVIQRTFAPS GGEQSGSDTD TDSGYGGELE KGDLRSEQPY FKSDHGRRFA VGERVSTIKQ ESEEPPTKKS RMQLSEEEGH FAGSDLMGSP FLGPHPHQPP FCLPFYLIPP SATAYLPMLE KCWYPTSVPV LYPGLNTSAA ALSSFMNPDK IPTPLLLPQR LPSPLAHSSL DSSALLQALK QIPPLNLETK D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May function as a transcriptional factor for neuronal differentiation. Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-ARNTL/BMAL1 complex. Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system.
  • 基因功能参考文献：
    1. Loss of Bhlhe40 expression is associated with impaired long term potentiation and long term depression at CA1 synapses. PMID: 29715265
    2. This study demonstrates that downregulation of DEC1 contributes to MPP(+) -induced neurotoxicity by suppressing PI3K/Akt/GSK3beta pathway. PMID: 28734031
    3. These results indicate that Bhlhe40 works as a cofactor of T-bet for enhancing IFN-gamma production in iNKT cells. PMID: 27226296
    4. Bhlhe40 expression identifies encephalitogenic Th cells and defines a PTX-IL-1-Bhlhe40 pathway active in experimental autoimmune encephalomyelitis. PMID: 26834156
    5. Collectively, the data obtained in this study suggest that DEC1 is a novel negative regulator of hepatic FGF21 expression PMID: 26697751
    6. establishes a crucial role of Bhlhe40 in mediating the repressive effect of hypoxia on myogenic differentiation and suggests that inhibition of Bhlhe40 or p53 may facilitate muscle regeneration after ischemic injuries PMID: 26468276
    7. Data show that the bHLH transcription factors SHARP1 and SHARP2 are involved in cognitive processing by controlling insulin-like growth factor II (Igf2) expression and associated signaling cascades. PMID: 26100875
    8. Data indicate that bHLH transcription factors Bhlhe40 interacts with PGC-1alpha protein (PGC-1alpha) and represses its transcriptional activity. PMID: 25963661
    9. abnormal sleep and certain (endo)phenotypes of psychiatric diseases may be caused by common mechanisms involving components of the molecular clock including SHARP1 and SHARP2. PMID: 25340473
    10. Study demonstrates that DEC1 is involved in osteogenesis. PMID: 24397494
    11. Bhlhe40 is required positively regulates the production of GM-CSF and negatively regulates the production of IL-10 in T cells. PMID: 24699451
    12. we identify DEC1 as a critical transcriptional mediator in the activation of naive CD4(+) T cells that is required for the development of a T cell-mediated autoimmune disease. PMID: 23878307
    13. Bhlhe40 is a possible candidate diabetic modifier gene responsible for Dbm1 locus affecting diabetes and/or obesity through negatively controlling fatty acid oxidation in muscle. PMID: 23229312
    14. Stra13 protects muscle cells from oxidative damage. PMID: 19679564
    15. SUMO modification of Stra13 is required for repression of cyclin D1 expression and cellular growth arrest PMID: 22905217
    16. Stra13, Dec and Sharp bHLH repressors are dynamically regulated during mammary gland development and may function to regulate apoptosis PMID: 15108316
    17. Hand1 and Stra13 promote cell cycle exit and restrict cells towards the Trophoblast Giant cell fate. PMID: 15196947
    18. Stra13 is expressed during mouse endochondral bone development PMID: 15465485
    19. RORalpha suppresses adipogenic differentiation at a later stage of differentiation by RORE-mediated stimulation of Dec1 and Dec2 expression. PMID: 22244086
    20. DEC1 is one of the mediators that reset the pattern of PPARgamma2 expression in response to hypoxia. PMID: 22610404
    21. Results suggest a partially redundant and bidirectional regulatory function for Dec1/2 genes in transcriptional translational feedback loops and conservation of Per1-Dec synergism between vertebrate and invertebrate clocks. PMID: 22215611
    22. measured circadian locomotor behavior and clock gene expression in the SCN of Per2/Dec1/2 double- and triple-mutant mice to analyze the functional interaction of PER2 and DEC1/2 feedback on circadian pacemaker function in the SCN PMID: 22080784
    23. Dec1 upregulates CD25 expression during thymocyte development and increases the number of Treg cells in the periphery. PMID: 21057086
    24. a mechanism for regulation of adipogenesis by hypoxia PMID: 11879638
    25. Stra13 is a negative regulator of B lymphocyte activation PMID: 11890681
    26. Stra13 binds with high affinity to CACGTG class B E-box elements as a homodimer with preference for elements preceded by T and/or followed by A residues PMID: 12297495
    27. DEC1 is the first transcription factor that can promote both chondrogenic differentiation and terminal differentiation PMID: 12384505
    28. Dec1 and Dec2 are regulators of the mammalian molecular clock, and form a fifth clock-gene family. [dec1] PMID: 12397359
    29. Data indicate that Stra13 may play an important protective role against oxidative stress in podocytes. PMID: 12594185
    30. expression regulated by mSharp-1/DEC2 PMID: 12657651
    31. STRA13 has a role as a circadian output regulator in the periphery PMID: 14581485
    32. Dec1 and Dec2 are upregulated by clock protein PMID: 15038852
    33. Marked changes in the Dec1 and Dec2 expression suggest that CLOCK plays a major role in the expression of these genes in most tissues. PMID: 16267380
    34. Stra13 is involved in DNA-damage-induced apoptosis and indicate in tumorigenesis. PMID: 17347673
    35. Plays an important role in postnatal myogenesis by attenuating Notch signaling to reduce myoblast proliferation and promote myogenic differentiation. PMID: 17502421
    36. BHLHB2 is a new modulator of Bdnf transcription and neuronal excitability. PMID: 18234890
    37. These observations collectively support the notion that overexpression of Stra13 and Dec2 links hypoxia signalling to altered circadian clock gene expression. PMID: 18342625
    38. DEC1, along with DEC2, plays a role in the finer regulation and robustness of the molecular clock CLOCK/BMAL1 PMID: 18411297
    39. Sharp-1 and Sharp-2 have roles as negative regulators of the molecular clock PMID: 18648504
    40. These results indicate that ALK-SMAD3-Dec1 signalling provides an input pathway in the mammalian molecular clock. PMID: 19029909
    41. Hepatic DEC1 mediates the ligand-dependent LXR signal to regulate the expression of genes involved in the hepatic clock system and metabolism. PMID: 19032342

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: mmu:20893

    STRING: 10090.ENSMUSP00000032194

    UniGene: Mm.2436