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  4. Recombinant Mouse Chromogranin-A (Chga)

Recombinant Mouse Chromogranin-A (Chga)

  • 货号：
    CSB-YP005344MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005344MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005344MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005344MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005344MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ChgaChromogranin-A; CgA) [Cleaved into: Pancreastatin; Beta-granin; WE-14; Catestatin; GE-25; Serpinin-RRG; Serpinin; AL26); p-Glu serpinin precursor]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-463
  • 氨基酸序列
    LP VNSPMTKGDT KVMKCVLEVI SDSLSKPSPM PVSPECLETL QGDERILSIL RHQNLLKELQ DLALQGAKER AQQPLKQQQP PKQQQQQQQQ QQQEQQHSSF EDELSEVFEN QSPDAKHRDA AAEVPSRDTM EKRKDSDKGQ QDGFEATTEG PRPQAFPEPN QESPMMGDSE SPGEDTATNT QSPTSLPSQE HVDPQATGDS ERGLSAQQQA RKAKQEEKEE EEEEEAVARE KAGPEEVPTA ASSSHFHAGY KAIQKDDGQS DSQAVDGDGK TEASEALPSE GKGELEHSQQ EEDGEEAMVG TPQGLFPQGG KGRELEHKQE EEEEEEERLS REWEDKRWSR MDQLAKELTA EKRLEGEDDP DRSMKLSFRT RAYGFRDPGP QLRRGWRPSS REDSVEARSD FEEKKEEEGS ANRRAEDQEL ESLSAIEAEL EKVAHQLQAL RRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Strongly inhibits glucose induced insulin release from the pancreas.; Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines.; Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation. Pyroglutaminated (pGlu)-serpinin exerts an antiapoptotic effect on cells exposed to oxidative stress.
  • 基因功能参考文献：
    1. Data suggest that hybrid insulin peptides (HIPs), formed in insulin-secreting-cells by fusion of insulin C-peptide fragments to peptide fragments of chromogranin A or islet amyloid polypeptide, and reactivity of CD4+-T-lymphocytes to HIPs may act as biomarkers of autoimmunity in type 1 diabetes. PMID: 29976617
    2. Systemic CST knockout (CST-KO) mice on normal chow diet (NCD) ate more food, gained weight, and displayed elevated blood glucose and insulin levels. PMID: 29432123
    3. CgA is an important regulator for coordination of mitochondrial dynamics, secretory vesicular quanta and glucose-stimulated insulin secretion for optimal secretory functioning of beta-cells, suggesting a strong, CgA-dependent positive link between mitochondrial fusion and glucose-stimulated insulin secretion PMID: 28220294
    4. Increased myocardial CgA glycosylation and impaired CgA processing to catestatin in heart failure be considered detrimental because CST reduces diastolic Ca2+ leak via direct CaMKIIdelta inhibition. PMID: 28209766
    5. performance of CgA-deficient Chga-KO mice in treadmill exercise was impaired. CgA deficiency renders the muscle energy deficient, impairs performance in treadmill exercise and prevents regeneration after exercise-induced tissue damage. PMID: 27799464
    6. dilated mitochondrial cristae, endoplasmic reticulum and Golgi complex, as well as increased synaptic mitochondria, synaptic vesicles and glycogen granules in Chga-knockout mice compared to WT mice. PMID: 26572539
    7. the presence of ChgA and subsequent activation of ChgA-reactive T cells are essential for the initiation and development of autoimmune diabetes in NOD mice. PMID: 26608914
    8. Studied leptin and CST modulation of SGLT1 expression in hyperleptinemic type 2 diabetic mice. PMID: 26552046
    9. N-terminal additions to the WE14 peptide of chromogranin A create strong autoantigen agonists in type 1 diabetes. PMID: 26453556
    10. Data indicate that T-cell receptors that react to chromogranin A (ChgA) and islet amyloid polypeptide precursor (IAPP) autoantigens were impaired when the thymic stromal cells lacked thymus-specific serine protease (TSSP). PMID: 26209627
    11. the important roles of CgA and CgB in glucose and cardiovascular homeostasis. This study also unveils the existence of direct implications of Cgs in the control of behaviour and mood. PMID: 25257107
    12. Chromogranin A (10-19) and chromogranin A (43-52) were identified as antigens for autoreactive CD8(+) T cells in NOD.beta2m(null).HHD mice. PMID: 25958206
    13. Report QT/heart rate variability in a genomically "humanized" chromogranin a monogenic mouse model with hyperadrenergic hypertension. PMID: 24821335
    14. We probed the role of the chromogranin A-derived peptide pancreastatin (PST: CHGA(273-301)) by investigating the effect of diet-induced obesity (DIO) on insulin sensitivity of these mice. PMID: 25048197
    15. Findings indicate that peptides from the N-terminal region of chromogranin A (ChgA) are able to induce cellular and humoral immune responses in NOD mice. PMID: 24443235
    16. It is a neuroinflammatory factor,which activates interleukin-1beta in brain microglia. PMID: 23831373
    17. Chromogranin A contributes to a highly efficient system that directly mediates monoamine accumulation and exocytosis in large dense core secretory vesicles. PMID: 22415354
    18. Although chromogranins regulate the amine concentration in chromaffin cell secretory vesicles, they are not completely essential. PMID: 21990378
    19. serpinin, a novel chromogranin A-derived peptide, is secreted upon stimulation of corticotrophs and plays an important autocrine role in up-regulating protease nexin-1-dependent granule biogenesis PMID: 21436258
    20. study concludes that cardiac secretory granules contain Chga, Chgb and Scg2 and that Chga is processed to catestatin in murine heart PMID: 21052719
    21. Study propose that CGA may function in the CNS as a prohormone and granulogenic factor in some terminal fields, but also possesses as-yet unknown unique cellular functions within neuronal somata and dendrites. PMID: 20005907
    22. Mice displayed a direct Chga gene dose-dependent (copies/genome) activation of Chga expression in both plasma and adrenal glands. PMID: 20139771
    23. The exaggerated action of Ucn 2 to reduce BP in the Chga-/- mouse is mediated through inhibition of both catecholamine synthesis and secretion. PMID: 20668031
    24. These data suggest that RAGE signaling is capable of driving neuronal differentiation involving CREB activation and induction of chromogranin expression. PMID: 12167613
    25. Chromogranin A has a role in secretory granule biogenesis, but is less effective than chromogranin B PMID: 12902350
    26. Rescue of elevated BP to normalcy was achieved by humanization of Chga mice. PMID: 16007257
    27. essential role of CgA in regulating chromaffin dense core secretory granules biogenesis and catecholamine storage PMID: 16049171
    28. Increased expression of the other granin family members is likely to compensate for the Chga deficiency in Chga-null mice. PMID: 16556729
    29. Ablation of the Chga gene affected the islet volume, the composition, distribution and nuclear size of islet cell types and plasma insulin concentration. PMID: 18722481
    30. Full-length CgA as well as deletion constructs containing the N-terminal 77 residues generated granule-like structures in the cell periphery that co-localized with co-expressed SgII (secretogranin II) PMID: 18973469
    31. CHGA acts through the glomerular endothelium to regulate renal function. PMID: 19520754
    32. pancreastatin has a role in glucose homeostasis and demonstrates a novel pathway of insulin sensitivity in chromogranin A null mice PMID: 19706599
    33. Ablation of Chga expression results in global disturbances in autonomic function, both sympathetic and parasympathetic. PMID: 19819970

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. Secreted.
  • 蛋白家族：
    Chromogranin/secretogranin protein family
  • 数据库链接：

    KEGG: mmu:12652

    STRING: 10090.ENSMUSP00000021610

    UniGene: Mm.4137