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  4. Recombinant Mouse Cytochrome P450 7A1 (Cyp7a1)

Recombinant Mouse Cytochrome P450 7A1 (Cyp7a1)

  • 货号：
    CSB-YP720674MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720674MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720674MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720674MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720674MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cyp7a1; Cyp7Cytochrome P450 7A1; 24-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.26; CYPVII; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; EC 1.14.14.23
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-503
  • 氨基酸序列
    MMSISLIWGI AVVVSCCIWF IIGIRRRKVG EPPLDNGLIP YLGCALKFGS NPLEFLRAKQ RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPSDGN TTENINKTFN KTLQGDALCS LSEAMMQNLQ SVMRPPGLPK SKSAVWVTEG MYAFCYRVMF EAGYLTLFGK DISKTDSQRA FIQNNLDSFK QFDQVFPALV AGVPIHLFKT AHKARERLAE SLKHKNLYMR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQ MIRSPEAMKA ASEEVNGALQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAK TTFYRNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFELELV ESHTKCPPLD QSRAGLGILP PLNDIEFKYK LKH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (Probable). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.
  • 基因功能参考文献：
    1. vitamin D-deficiency resulted in down-regulation of liver Cyp7a1 and renal Oat3, conditions that are alleviated upon replenishment of cholecalciferol. PMID: 29243851
    2. Hypercholesterolemia in TALLYHO/jng mice might increase the Bile acids amounts via the interrupted Fxr/Fgf15/Fgfr4-mediated feedback regulation of Cyp7a1. PMID: 28843503
    3. although feeding LS-Xbp1(-/-) mice cholesterol did not increase CYP7A1 expression, serum C4 levels increased significantly up to levels similar to chow-fed Xbp1(fl/fl) mice and the total bile acid pool normalized. In conclusion, loss of hepatic XBP1 decreased the bile acid pool and CYP7A1 synthetic activity. PMID: 28039331
    4. Cyp7a1(-/-) mice have improved glucose sensitivity with reduced liver triglycerides and fecal bile acid excretion, but increased fecal fatty acid excretion and respiratory exchange ratio (RER) when fed a high-fat/high-cholesterol diet PMID: 27146480
    5. Current studies suggest that increased Cyp7a1 expression and bile acid synthesis ameliorate hepatic inflammation through activation of FXR PMID: 27534992
    6. HSF-1 ablation not only eliminates heat shock response, but it also transcriptionally up-regulates CYP7A1 and MDR1/P-gp axis in WD-diet fed HSF-1(-/-)/LDLr(-/-) mice to reduce atherosclerosis. PMID: 27131506
    7. Mice deficient in cholesterol 7 alpha-hydroxylase, supplemented with chenodeoxycholic acid, have altered liver and intestinal bile acids. PMID: 25447797
    8. Activation of the VDR represses hepatic SHP to increase levels of CYP7A1 and reduce cholesterol. PMID: 24365583
    9. Cholesterol lowering properties of oats involve increased production of bile acids via the classic pathway with up-regulation of CYP7A1 and CYP8B1. PMID: 23262749
    10. A CYP7A1/SREBP2/miR-33a axis plays a critical role in regulation of hepatic cholesterol, bile acid, and fatty acid synthesis. PMID: 23536474
    11. Ileal Fibroblast Growth Factor-15 (FGF15) expression was significantly reduced in Asbt(-/-)apoE(-/-) mice and was inversely correlated with expression of hepatic cholesterol 7-hydroxylase (Cyp7a1). PMID: 23880190
    12. HNF4alpha and LRH-1 promote active transcription histone marks on the Cyp7a1 promoter that are reversed by FGF19 in a SHP-dependent manner PMID: 23038264
    13. these data suggest that FGF7 is a novel regulator of CYP7A1 expression in hepatocytes and may prevent hepatocytes from accumulating toxic bile acids during liver injury and fibrosis. PMID: 22713451
    14. Results indicate that although PPARgamma activation increased PCSK9 expression, PPARgamma activation induced LDLR and CYP7A1 expression that enhanced LDL cholesterol metabolism. PMID: 22593575
    15. Cyanidin-3-O-beta-glucoside consumption reduced hypercholesterolemia, promoted fecal bile acid excretion and upregulated hepatic cholesterol 7a-hydroxylase expression (CYP7A1). PMID: 22495986
    16. Chitosan oligosaccharides promote reverse cholesterol transport and expression of scavenger receptor BI and CYP7A1 in mice PMID: 22302708
    17. Although gene expressions of HMG-CoAR and CYP7A1 in diabetic buckwheat sprout-fed groups were higher than in the diabetic control group, the elevation of mRNA level of CYP7A1 was greater than that of HMG-CoAR. PMID: 21535603
    18. Liver receptor homolog-1 is critical for adequate up-regulation of Cyp7a1 gene transcription and bile salt synthesis during bile salt sequestration. PMID: 21391220
    19. induction of CYP7A1 expression and expansion of a hydrophobic bile acid pool stimulate cholesterol conversion into bile acids, de novo cholesterol synthesis, and biliary free cholesterol secretion, without increasing intestinal cholesterol absorption. PMID: 21319191
    20. lack of stimulation of gene by dietary cholesterol PMID: 11967256
    21. interaction of peroxisome proliferator-activted receptor alpha with liver x receptor alpha antagonizes the stimulatory effect of their respective ligands on the murine cholesterol 7alpha-hydroxylase gene promoter PMID: 12117567
    22. transcription suppressed by alpha1-antitrypsin peptide via interaction with AFP PMID: 12223476
    23. Feedback regulation of the rate-limiting biosynthetic enzyme CYP7A1 is lost in Cyp8b1(-/-) mice, causing expansion of the bile acid pool and alterations in cholesterol metabolism. PMID: 12393855
    24. bile acids suppress transcription of the gene (CYP7A1) encoding cholesterol 7alpha-hydroxylase, the rate-limiting enzyme in bile acid biosynthesis PMID: 12865425
    25. CYP7a1 synthesis is not stimulated by leptin PMID: 12917427
    26. CYP7A1 is activated by PGC-1alpha PMID: 14522988
    27. dietary cholesterol regulates bile acid pool size, fecal bile acid excretion, and plasma cholesterol independently of Cyp7a1 activity PMID: 14762172
    28. LC omega-3 fatty acid-rich diet upregulated transcriptional level of Cyp7a1, D-site binding protein, and liver X receptor-alpha. PMID: 14960319
    29. Cyp7a1 deficiency in APOE*3-Leiden mice decreases the VLDL particle production rate, as a consequence of a strongly reduced bile acid biosynthesis, leading to a decrease in plasma triglycerides. PMID: 14962946
    30. Expression of gene Cyp7a1 indicates hepatic differentiation in embryonid bodies. PMID: 15569160
    31. Hepatic neutral cytosolic cholesteryl ester hydrolase decreased mRNAs for cholesterol 7alpha-hydroxylase, sterol 27-hydroxylase, and HMG-CoA reductase on day 7 after transfection, coinciding with peak hepatic free cholesterol concentrations PMID: 15825828
    32. LDL receptor-deficient mice expressing the CYP7A1 transgene exhibited complete resistance to diet-induced hypercholesterolemia and to the accumulation of cholesterol in the liver. PMID: 16609145
    33. Identification of two new functional thyroid hormone receptor-binding sites in the CYP7A1 5' flanking sequence located 3 kb upstream from the transcription start site. PMID: 16899449
    34. Strongly reduced bile acid synthesis in Cyp7a1-/-.APOE*3-Leiden mice leads to increased plasma HDL-cholesterol levels, as related to an increased hepatic expression of ABCA1. PMID: 17008588
    35. The expression pattern of Cyp7a1 was studied in hepatocytes. PMID: 17237956
    36. SREBP-1c functions as a non-DNA-binding inhibitor and mediates, in part, suppression of Cyp7a1 by blocking functional interaction of HNF-4 and PGC-1alpha PMID: 17636037
    37. PGC-1alpha is an important co-activator for LRH-1 and that SHP targets the interaction between LRH-1 and PGC-1alpha to inhibit CYP7A1 expression. PMID: 18385139
    38. The mouse Cyp7A1 promoter, used for trasient transfection of fluorescent gene reporters in ES spontaneously differentiated into hepatocytes, was better than the albumin promoter for cell sorting. PMID: 18942772
    39. Results suggest that CYP7a1 suppression is required for liver protection after 70% partial hepatectomy and there are two distinct phases of CYP7a1 gene regulation during liver regeneration. PMID: 19056864
    40. The present findings demonstrate that IL-1alpha and IL-1beta are positive regulators for the Cyp7a1 gene in steady-state mice and that Cyp7a1 is one of the factors that mediate the difference in serum total cholesterol level between sexes. PMID: 19101514
    41. the AU-rich RNA binding-protein Apobec-1 mediates post-transcriptional regulation of Cyp7a1 expression and influences susceptibility to diet-induced gallstone formation PMID: 19386592
    42. Impaired regulation of FoxO1 may cause down-regulation of CYP7A1 gene expression and contribute to dyslipidemia in insulin resistance. PMID: 19463968

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: mmu:13122

    STRING: 10090.ENSMUSP00000029905

    UniGene: Mm.57029