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  4. Recombinant Mouse Cholecystokinin (Cck)

Recombinant Mouse Cholecystokinin (Cck)

  • 货号：
    CSB-YP004771MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004771MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004771MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004771MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004771MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cck
  • Uniprot No.：
  • 别名：
    CckCholecystokinin; CCK) [Cleaved into: Cholecystokinin-33; CCK33); Cholecystokinin-12; CCK12); Cholecystokinin-8; CCK8)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    21-115
  • 氨基酸序列
    QPVVPAEATD PVEQRAQEAP RRQLRAVLRT DGEPRARLGA LLARYIQQVR KAPSGRMSVL KNLQSLDPSH RISDRDYMGW MDFGRRSAED YEYPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  • 基因功能参考文献：
    1. Study shows that independent of the neurochemical content, cholecystokinin/type 1 cannabinoid receptor-expressing interneurons have similar physiological and morphological properties, providing an endocannabinoid-sensitive synaptic inhibition onto the amygdalar principal neurons. PMID: 28391401
    2. In summary, our study is the first to indicate that CCK/CCK-AR pathway is critical and protective against liver I/R injury. The activation of this pathway not only prevents hepatocellular apoptosis, but also reduces inflammatory response by suppressing NF-kappaB. PMID: 28521244
    3. CCK/GLP-1 play contributory roles in anorexia induction by trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol and neosolaniol. PMID: 28964791
    4. GPR120-induced incretin glucse-dependent insulinotropic polypeptide secretion is indirectly mediated by cholecystokinin. PMID: 28324023
    5. Medullary interstitial cells respond to body fluid expansion by CCK release for feedback regulation of the late proximal tubular reabsorption. PMID: 28298361
    6. results suggest that normal integration of CCK+ basket cells in cortical networks is key to support spatial coding in the hippocampus. PMID: 28394324
    7. PC7 has a critical role in normal processing of cholecystokinin in mouse brain PMID: 27923657
    8. These studies reemphasize the beneficial effects imparted by co-administration of obestatin and CCK8 and their potential use towards countering obesity. PMID: 27032885
    9. new information on the cell specific localization of NUCB2/nesfatin-1 in the intestinal mucosa, and a novel function for nesfatin-1 in modulating intestinal CCK and PYY expression and secretion PMID: 26920055
    10. Results showed that CCK is important for lipid transport and energy expenditure to control body weight in response to dietary lipid feeding PMID: 26171590
    11. SP1 results in a CCK response deficiency that may contribute to the increased meal size and overall hyperphagia in synphillin-1 transgenic mice PMID: 26569394
    12. active GLP-1 produced in the islet stimulates cholecystokinin production and secretion in a paracrine manner via cyclic AMP and CREB. PMID: 25984632
    13. Data (including data from studies using transgenic mice) suggest that enhanced Cck expression in pancreatic beta-cells protects these cells from the cell-withering effects of aging, apoptotic stress, and (streptozotocin-induced) diabetes type 2. PMID: 26394663
    14. total Ca(2+) mobilization evoked by CCK-8 was attenuated by a 30% in the presence of 100 microM melatonin compared with the responses induced by CCK-8 alone. PMID: 25084987
    15. Endogenous cholecystokinin is in part responsible for the development and progression of pancreatic cancer. PMID: 25058882
    16. octapeptide exerts a direct effect on T cells, which is dependent on cholecystokinin-2 receptor PMID: 24704498
    17. Data demonstrate that the ERK pathway is required for CCK-stimulated pancreatic adaptive growth. PMID: 25104499
    18. I cells in duodenum are enriched in expression of CCK and ghrelin. PMID: 25004095
    19. Data show the transcriptional activation of the cholecystokinin gene by DJ-1 through interaction of DJ-1 with RREB1 and the effect of DJ-1 on the cholecystokinin level. PMID: 24348900
    20. This study concluded that CCK is a mediator of dietary fat-associated pancreatic cancer, and it is also involved in the invasiveness of pancreatic tumors. PMID: 24817409
    21. CCK enhances cholesterol absorption by activation of a pathway involving CCK1R/CCK2R, Gbetagamma, PI3K, Akt, Rab11a, and NPC1L. PMID: 24692543
    22. mouse corneal afferent sensory neurons expressed CCK and GAST, and the CCK1R receptors. PMID: 24576871
    23. Gastric PAI-1 modulates vagal effects of cholecystokinin. PMID: 23816469
    24. ILDR1 regulates CCK release through a mechanism dependent on fatty acids and lipoproteins. PMID: 23863714
    25. hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
    26. CD36 is a major mediator of FA-induced release of CCK and secretin. These peptides contribute to the role of CD36 in fat absorption and to its pleiotropic metabolic effects. PMID: 23233532
    27. a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin PMID: 23064014
    28. peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
    29. These data suggest that a mixture of guar gum and fructo-oligosaccharide can maintain its appetite suppressant effect in fatty media. PMID: 23054308
    30. Our data demonstrate that CCK expressed in the basolateral amygdala represents a key brain substrate for anxiogenic and depressant effects of the peptide. PMID: 22613736
    31. expression of CCK is increased in hippocampal pyramidal cells in mice with recurrent, spontaneous seizures PMID: 22155653
    32. Data suggest that CCK acts to increase glutamatergic transmission to the preproglucagon-positive neurons; this action appears to involve activation of alpha1-adrenergic receptors. PMID: 21885869
    33. Simotang enhances the gastrointestinal motility in chronically stressed mice by regulating the serum motilin level and the expression of cholecystokinin. PMID: 21472126
    34. Gene expressions of ghrelin, PYY, and CCK was increased in the gastrointestinal tract of the hyperphagic intrauterine growth restriction rat offspring. PMID: 21264794
    35. Cholecystokinin signalling is not involved directly in light-induced resetting of the suprachiasmatic nucleus or in regulating its function. PMID: 20731710
    36. Short-term DeltaFosB overexpression increases both Cck promoter activity and gene expression. PMID: 20226774
    37. Conjugated linoleic acid isoforms are particularly potent CCK secretagogues, which also boost intracellular stores of CCK. PMID: 20352619
    38. CCK is up-regulated by islet cells during obesity and functions as a paracrine or autocrine factor to increase beta-cell survival and expand beta-cell mass to compensate for obesity-induced insulin resistance. PMID: 20534724
    39. CCK is involved in regulating the metabolic rate and is important for lipid absorption and control of body weight in mice placed on a high-fat diet. PMID: 20117110
    40. Lack of CCK induces gallbladder hypomotility that prolongs the residence time of excess cholesterol in the gallbladder, leading to rapid crystallization and precipitation of solid cholesterol crystals. PMID: 19836465
    41. role of anorectic effects possibly by enhancing release of pancreatic amylin PMID: 12576089
    42. In whole area of hippocampus, NPY-, SOM- (somatostatin), CCK-, and VIP-positive neurons accounted for about 31%, 17%, 7%, and 8% of GABAergic neurons, respectively. PMID: 12746872
    43. Intense cholecystokinin immunoreactivity is found in the mossy fiber pathway (stratum lucidum and dentate hilus) and in the inner molecular layer of the dentate gyrus. PMID: 14643757
    44. Lack of gastrin impairs gastrin-enterochromaffin-like cell axis, whereas lack of gastrin and CCK impairs both hormonal pathways. In gastrin-CCK double-knockout mice, acid secretion is controlled by cholinergic vagal stimulation, normalizing acid output. PMID: 14762785
    45. In this review, tissue-specific processing of cholecystokinin precursor in brain and gut to a large extent can be explained by the roles of prohormone convertases 1 and 2. PMID: 15075450
    46. These results indicate that CCK provides an inhibitory influence on GnRH-1 neuronal migration, contributing to the appropriate entrance of these neuroendocrine cells into the brain, and thus represent the first report of a developmental role for CCK PMID: 15152034
    47. Psychological stress inhibits the small intestinal transit, probably by down-regulating CCK and up-regulating VIP expression in small intestine. PMID: 15655834
    48. Both gastrin and combined gastrin-cholecytokinin deficiency reduced the gastric IAPP and Peptide Yy expression. PMID: 16002530
    49. prohormone convertase 2 is important for cholecystokinin processing PMID: 16174778
    50. results provide the first direct evidence that prohormone convertase 5 (PC5) is involved in CCK processing PMID: 16266771

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Gastrin/cholecystokinin family
  • 数据库链接：

    KEGG: mmu:12424

    STRING: 10090.ENSMUSP00000035120

    UniGene: Mm.2619