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  4. Recombinant Mouse Chitinase-3-like protein 3 (Chi3l3)

Recombinant Mouse Chitinase-3-like protein 3 (Chi3l3)

  • 货号：
    CSB-YP518578MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP518578MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP518578MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP518578MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP518578MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Chil3
  • Uniprot No.：
  • 别名：
    Chil3; Chi3l3; Ym1Chitinase-like protein 3; EC 3.2.1.52; Beta-N-acetylhexosaminidase Ym1; Chitinase-3-like protein 3; ECF-L; Eosinophil chemotactic cytokine; Secreted protein Ym1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-398
  • 氨基酸序列
    YQLMCYYTS WAKDRPIEGS FKPGNIDPCL CTHLIYAFAG MQNNEITYTH EQDLRDYEAL NGLKDKNTEL KTLLAIGGWK FGPAPFSAMV STPQNRQIFI QSVIRFLRQY NFDGLNLDWQ YPGSRGSPPK DKHLFSVLVK EMRKAFEEES VEKDIPRLLL TSTGAGIIDV IKSGYKIPEL SQSLDYIQVM TYDLHDPKDG YTGENSPLYK SPYDIGKSAD LNVDSIISYW KDHGAASEKL IVGFPAYGHT FILSDPSKTG IGAPTISTGP PGKYTDESGL LAYYEVCTFL NEGATEVWDA PQEVPYAYQG NEWVGYDNVR SFKLKAQWLK DNNLGGAVVW PLDMDDFSGS FCHQRHFPLT STLKGDLNIH SASCKGPY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.
  • 基因功能参考文献：
    1. The soluble antigen from A. cantonensis could promote the Chil3 expression in macrophage and microglial cell lines induced by interleukin-13. PMID: 27256220
    2. A significant increase of CHI3L3 mRNA expression was observed in the spinal cord of experimental autoimmune encephalitis mice compared to controls during the inflammatory phase. PMID: 26079949
    3. neutrophil-derived Ym1 protein uptake distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing PMID: 25307347
    4. These data indicate that helminth-induced immunomodulation occurs independently of changes in the microbiota but is dependent on Ym1. PMID: 25082704
    5. These results showed that Ym1 was most highly expressed in the brains and cerebrospinal fluid of the Angiostrongylus cantonensis infected mice along with the process of inflammation. PMID: 23703548
    6. findings implicate heparanase in normal processing of heparan sulfate in macrophages, and indicate that heparanase regulates intracellular Ym1 accumulation and crystal formation in airways. PMID: 20226534
    7. was shown to be a neutrophil granule protein and to have weak beta-N-acetylglucosaminidase activity, indicating that it might contribute to the digestion of glycosaminoglycans. PMID: 11733538
    8. TH2 cytokines and allergic challenge induce expression in macrophages by a STAT6-dependent mechanism PMID: 12215441
    9. data show ECF-L is a previously unknown factor that is a potent mediator of osteoclast formation, which acts at the later stages of osteoclast formation and enhances the effects of RANKL PMID: 12854845
    10. crystal structure of Ym1 at 1.31 A resolution PMID: 15522777
    11. Constitutively expressed in bone marrow cells and gradually decreased in the presence of IL-3. PMID: 16174095
    12. These results indicate that ECF-L may play a critical role in allergic inflammation and bronchial asthma. PMID: 16528013
    13. Results suggest that modulation of Ym1 production by dendritic cells identifies a previously undescribed mechanism of Th2 polarization by statin. PMID: 16682645
    14. Quantitative RT-PCR demonstrated a 976-fold increase of Chi3l4 mRNA expression and a 24-fold increase of Chi3l3 mRNA expression in the skin of cpdm/cpdm mice. PMID: 16984263
    15. ECF-L enhances RANKL and 1,25-(OH)2D3-induced OCL formation by increasing adhesive interactions between OCL precursors through increased expression of ICAM-1 and LFA-1. PMID: 16996813
    16. Bronchoalveolar lavage fluid protein Ym1 is cleaved by matrix metalloproteinases 2 and 9 and is essential for regulating inflammatory pathways in experimental asthma. PMID: 17082650
    17. Glucocorticoid receptor-null lungs included Chi3l3 (11-fold, P < 0.05), a macrophage inflammatory response gene and Ela1 (9.4-fold, P < 0.05), an extracellular matrix remodeling enzyme. PMID: 17901120
    18. Production of Ym1/2 in response to interleukin-13 promotes T helper type (Th)2 cell cytokine production and allergic airway inflammation by inhibiting the production of 12(S)-hydroxyeicosatetraenoic acid (HETE) by 12/15(S)-lipoxygenase (LOX). PMID: 19380786
    19. Crystalline macrophage pneumonia is due to overexpression of ym1 (now chi3I3) in lung macrophages which causes illness and death in mice. PMID: 19095958
    20. Hyalinosis is due to the overexpression of ym1 (now chi3I3) in cells and tissues, often associated with disease in that tissue. PMID: 11141507
    21. Eosinophilic crystalline pneumonia is due to over expression of ym1 in lung macrophages, which causes illness and death in mice. PMID: 16966445

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  • 亚细胞定位：
    Secreted. Rough endoplasmic reticulum lumen. Nucleus envelope. Cytoplasm. Cytoplasmic granule. Note=Predominantly localizes to the lumen of rough endoplasmic reticulum (rER) and nuclear envelope in alveolar macrophages. Localizes to the dilated lumen of rER in immature neutrophils in spleen and in cytoplasmic granules in peritoneal neutrophils. Detected in needle-shaped crystals present in the cytoplasm of bone marrow macrophages.
  • 蛋白家族：
    Glycosyl hydrolase 18 family, Chitinase class II subfamily
  • 组织特异性：
    Expressed in peritoneal cavity macrophages and in peritoneal and bone marrow-derived neutrophils. Abundantly expressed in bone marrow, with moderate levels detected in gastric antrum, spleen and in alveolar macrophages in lung. Not detected in brain, hear
  • 数据库链接：

    KEGG: mmu:12655

    STRING: 10090.ENSMUSP00000053923

    UniGene: Mm.387173