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  4. Recombinant Mouse Cerebellin-1 (Cbln1)

Recombinant Mouse Cerebellin-1 (Cbln1)

  • 货号：
    CSB-YP879503MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP879503MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP879503MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP879503MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP879503MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cbln1Cerebellin-1; Brain protein D3; Precerebellin) [Cleaved into: Cerebellin; CER); [des-Ser1]-cerebellin; des-Ser1-cerebellin)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-193
  • 氨基酸序列
    QNETEPIVL EGKCLVVCDS NPTSDPTGTA LGISVRSGSA KVAFSAIRST NHEPSEMSNR TMIIYFDQVL VNIGNNFDSE RSTFIAPRKG IYSFNFHVVK VYNRQTIQVS LMLNGWPVIS AFAGDQDVTR EAASNGVLIQ MEKGDRAYLK LERGNLMGGW KYSTFSGFLV FPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the matching and maintenance of pre- and post-synaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses. Plays a role as a synaptic organizer that acts bidirectionally on both pre- and post-synaptic components. On the one hand induces accumulation of synaptic vesicles in the pre-synaptic part by binding with NRXN1 and in other hand induces clustering of GRID2 and its associated proteins at the post-synaptic site through association of GRID2. NRXN1-CBLN1-GRID2 complex directly induces parallel fiber protrusions that encapsulate spines of Purkinje cells leading to accumulation of GRID2 and synaptic vesicles. Required for CBLN3 export from the endoplasmic reticulum and secretion. NRXN1-CBLN1-GRID2 complex mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis. Essential for long-term maintenance but not establishment of excitatory synapses. Inhibits the formation and function of inhibitory GABAergic synapses in cerebellar Purkinje cells.; The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
  • 基因功能参考文献：
    1. Results show that Cbln1, Cbln2, and Cbln4 are abundantly expressed in nearly all brain regions, but exhibit strikingly different expression patterns and developmental dynamics. Using newly generated knockin reporter mice for Cbln2 and Cbln4, we find that Cbln2 and Cbln4 are not universally expressed in all neurons, but only in specific subsets of neurons. PMID: 28714144
    2. results suggest that gene and seizure interactions in ventral tegmental area glutamatergic neurons impair sociability by downregulating Cbln1, a key node in the expanding protein interaction network of autism genes PMID: 28297715
    3. Despites its well known role in motor coordination and motor learning, whether and how the cerebellum is involved in cognitive functions remains less clear. Cerebellin 1 (Cbln1) is highly expressed in the cerebellum and serves as an essential synaptic organizer. Although genes encoding Cbln1 and its receptor are associated with many psychiatric disorders, it remains unknown whether such cognitive impairments are caused by PMID: 27852787
    4. amplitude and frequency of mIPSCs were increased in cbln1-null cerebellar slices. PMID: 24467251
    5. This study provides evidence for involvement of Cbln1 in nociception and provides a platform for continued exploration of involvement of novel neuropeptides in the regulation of nociceptive transmission PMID: 24749662
    6. that neurons expressing Cblns 1, 2, and 4 tend to occupy different laminar positions within the dorsal spinal cord PMID: 23853053
    7. extracerebellar structures or remaining PF-PC synapses in cbln1(-/-) mice may be sufficient for the expression, maintenance, and extinction of its memory trace. PMID: 24298240
    8. Cbln1 released from parallel fiber Purkinje cells induces dynamic structural changes in parallel fibers. PMID: 23141067
    9. [review] The Cbln1-GluRdelta2 receptor complex is located at the cleft of parallel fiber-Purkinje cell synapses and bidirectionally regulates both presynaptic and postsynaptic differentiation. PMID: 20535596
    10. GluRdelta1 stimulated inhibitory synapse formation in the presence of Cbln1 or Cbln2 PMID: 22191730
    11. Data show that Cbln1 and Cbln2, but not Cbln4, specifically bind to neurexin 1alpha and -beta and induce synaptogenesis in cerebellar, hippocampal and cortical neurons in vitro. PMID: 21410790
    12. These results suggest that Cbln1 and Cbln2 play a role in synapse formation of cortical neurons by interacting with NRXNs containing S4. PMID: 21356198
    13. Otolin 1 interacts with otoconial matrix protein Otoconin-90 and Cerebellin-1 in the inner ear. PMID: 20856818
    14. Our observation that loss of Cbln1 leads to increased numbers of striatal medium spiny neurons' dendritic spines suggests that modulating thalamostriatal Cbln1 may be a novel means of improving symptoms or slowing progression in Parkinson's disease. PMID: 20503425
    15. the flap loop (Arg321-Trp339) in the NTD of GluD2 (GluD2-NTD) was identified as a crucial region for the binding to Cbln1 and the induction of presynaptic differentiation. PMID: 20599760
    16. Results suggest that GluRdelta2 mediates cerebellar synapse formation by interacting with presynaptic NRXNs through Cbln1. PMID: 20537373
    17. study found that Cbln1 binds directly to the N-terminal domain of glutamate receptor delta2 (GluD2); results indicate that the Cbln1-GluD2 complex is a unique synapse organizer that acts bidirectionally on both pre- and postsynaptic components PMID: 20395510
    18. the cleavages required to produce cerebellin influence the subunit stoichiometry of Cbln1 complexes PMID: 16135095
    19. In the developing brain, Cbln1 mRNA appeared as early as embryonic day 10-13, and exhibited transient up-regulation during the late embryonic and neonatal periods. PMID: 16930405
    20. Cbln1 is required for endoplasmic reticulum export and secretion of Cbln3. PMID: 17030622
    21. During development of cbln1-lacZ mice, beta-galactosidase staining was first observed in proliferating granule cell precursors prior to synaptogenesis and thereafter in maturing and adult granule cells. PMID: 18001291
    22. Cbln1 is a unique synapse organizer that is required not only for the normal development of parallel fiber-Purkinje cell synapses but also for their maintenance in the mature cerebellum both in vitro and in vivo. PMID: 18524896
    23. These results indicate that Cbln1, released from granule cells as hexamers, specifically binds to a putative receptor located at the postsynaptic sites of parallel-Purkinje cell synapses. PMID: 19200061
    24. immunohistochemical localization suggests the basis for anatomical convergence of the Cbln-GluRdelta2 signaling pathway in parallel fiber-Purkinje cell synapses PMID: 19250438
    25. Data show that Cbln1 undergoes anterograde transport from granule cells to Purkinje cells and Bergmann glia and enters the endolysosomal trafficking system, suggesting that Cbln1 exerts its activity on or within Purkinje cells and Bergmann glia. PMID: 19344768
    26. Activity-dependent downregulation of Cbln1 may serve as a new presynaptic mechanism by which parallel fibers-Purkinje cell synapses adapt to chronically elevated activity, thereby maintaining homeostasis. PMID: 19403810

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  • 亚细胞定位：
    Secreted. Cell junction, synapse, postsynaptic cell membrane.
  • 组织特异性：
    Expressed and secreted by presynaptic neurons, such as cerebellar granule cells. Following secretion, the protein binds to GRID2 on the postsynaptic Purkinje cell membranes. Expressed at the highest level in the cerebellar cortex. High levels are also see
  • 数据库链接：

    KEGG: mmu:12404

    STRING: 10090.ENSMUSP00000034076

    UniGene: Mm.4880