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  4. Recombinant Mouse Cellular tumor antigen p53 (Tp53)

Recombinant Mouse Cellular tumor antigen p53 (Tp53)

In Stock
  • 货号：
    CSB-EP024077MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tp53; P53; Trp53; Cellular tumor antigen p53; Tumor suppressor p53
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    59.2kDa
  • 表达区域：
    1-387aa
  • 氨基酸序列
    MEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
  • 基因功能参考文献：
    1. results indicate that miR-148b-3p contributes to the regulation of hypoxia/reoxygenation-induced cardiomyocyte apoptosis in vitro through targeting SIRT7 and modulating p53-mediated pro-apoptotic signaling PMID: 30308185
    2. Data provide evidence that p53 enhances NOXA-induced apoptosis when autophagy is inhibited. PMID: 29758299
    3. The p53 is critical in activation of T lymphocytes not only by regulating proliferation and apoptosis but also because of its effective function in the pathogenesis of septic complications. PMID: 30453300
    4. 133p53 promotes tumour invasion via IL-6 by activation of the JAK-STAT and RhoA-ROCK pathways. PMID: 29343721
    5. Results show an association of PD with p53 and active caspase-3 overexpression and beta-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD. PMID: 30135418
    6. These results show that ischemic preconditioning increased neuronal MDM2 protein levels, which prevented ischemia-induced p53 stabilization and neuronal death. PMID: 29371613
    7. In studies of lymphomagenesis, mutant TRP53 drives tumorigenesis primarily through dominant-negative effects, which modulates wild-type TRP53 function in a manner advantageous for neoplastic transformation. PMID: 30366906
    8. p53 deteriorated cardiac functions and cardiac hypertrophy, apoptosis, and fibrosis by partially inhibition of HIF1alpha and VEGF. PMID: 29729274
    9. MDMX is an important regulator of p53 DNA binding, which complements the role of MDM2 in regulating p53 level. PMID: 29581299
    10. the disruption of Mdm2/p53 interaction affects the early-embryonic otic progenitor cells and their descendants. PMID: 28181574
    11. Deletion of NF1 leads to mutant oligodendrocyte precursor cell (OPC) expansion through increased proliferation and decreased differentiation, the deletion of p53 impairs OPC senescence. Signaling analysis showed that, while PI3K and MEK pathways go through stepwise over-activation, mTOR signaling remains at the basal level in pre-transforming mutant OPCs but is abruptly up-regulated in tumor OPCs. PMID: 29392777
    12. findings uncover a new function of p53 in the regulation of Akt signaling and reveal how p53, ASS1, and Akt are interrelated to each other. PMID: 28560349
    13. Rlip deficiency suppresses spontaneous malignancy in p53(-/-) mice. PMID: 29572430
    14. Obtained results suggest that Wip1 deficiency sensitizes cells to sodium butyrate and to MEK/ERK inhibitors independently from Wip1 main target protein - p53. Data acquired give insights into role of Wip1 in cellular responses to treatment with HDAC and MEK/ERK inhibitors. PMID: 30188093
    15. CYLD removes K48-linked ubiquitin chains from p53 indirectly by cleaving K63 chains, suggesting that p53 is decorated with complex ubiquitin chains. PMID: 27561390
    16. tumor suppressor p53 functions as an essential antiviral molecule against Japanese encephalitis virus PMID: 27956051
    17. Results show that p53 expression and mutational status do not interfere with CES2 expression in colorectal neoplasm. PMID: 29651325
    18. the beta-catenin C-terminus indirectly represses p53, and this function is essential for embryogenesis. PMID: 27499244
    19. Results suggest that the chronic inflammation due to constitutive overexpression of TNF is not sufficient to significantly accelerate tumorigenesis driven by the loss of p53. PMID: 28079895
    20. the MDM2-p53-PC signalling axis links mitochondrial metabolism to insulin secretion and glucose homeostasis, and could represent a therapeutic target in diabetes. PMID: 27265727
    21. Comparison of lineage-related p53 knockout and wild-type clones reveals a minor role of p53 in suppressing cell expansion in lung adenomas. In contrast, p53 loss promotes both the initiation and expansion of low-grade pancreatic intraepithelial neoplasia, likely through differential expression of the p53 regulator p19ARF. PMID: 27585860
    22. The neuroprotective effects of p53/microRNA22 may regulate inflammation and apoptosis in in subarachnoid hemorrhage. PMID: 29336471
    23. wild-type p53 exerts a dominant tumor-suppressive effect on mutant tumors, as all genotypes were similarly sensitive to its restoration in vivo PMID: 28790158
    24. Data demonstrate a causative role for TRP53 mutants in development of chemoresistant lung cancer. PMID: 28847987
    25. Activation of p53 blocks neural differentiation of miRNA-deficient pluripotent stem cells. PMID: 29141234
    26. p53 mediates the regulation of bile acid disposition and attenuates cholic acid-induced cholestasis in mice PMID: 28910492
    27. Data demonstrates that p53 inhibits OSX binding to their responsive Sp1/GC-rich sites in the promoters of their osteogenic target genes, such as IBSP or COL1A1. PMID: 28777372
    28. A summary of current knowledge of p53-mediated tumor-suppressive mechanisms gleaned from in vivo studies in mouse models (review). PMID: 29099489
    29. Increased Cdc7-dependent replication initiation is a hallmark of p53 gain-of function mutations in lung adenocarcinoma. PMID: 28887320
    30. Aging mouse models have revealed the complexity of the p53-Mdm2 axis and have solidly placed the p53 network as being key to many aspects of both pathological aging conditions and normal aging (review). PMID: 29192902
    31. In myoblasts silenced for the expression of muscle regulatory transcription factor MyoD, tumor suppressor p53 binding to the PUMA promoter is diminished in response to culture in differentiation medium. PMID: 28918507
    32. Study provides evidence that high expression of CLDN6 confers chemoresistance on breast cancer which is mediated by GSTP1, the activity of which is regulated by p53. PMID: 29116019
    33. AKI was associated with the upregulation of several known p53 target genes. This was attenuated in p53-KO mice. In human renal tubular epithelial cell line, vancomycin induced p53 accumulation. p53 was associated with the development of VAN-induced AKI through upregulation of miR-192-5p. PMID: 27941921
    34. Recent work in a TP53(-/-)BRCA1-mutant murine breast cancer model indicates that double blockade with two immune checkpoint inhibitors increases the number of tumor-infiltrating lymphocytes and overall survival after DNA damaging chemotherapy, whereas single blockade does not PMID: 29120748
    35. Study reports a high-incidence, low-variation spontaneous mouse model of spondyloarthropathy that delineates how the combination of inflammatory cytokine interleukin-27 (IL-27) signaling deficiency and mitogenic signaling (mutant p53R172H) in vivo, leads to bone loss in the vertebral bodies and ossification of the cartilage in the intervertebral discs. PMID: 29494633
    36. Knockdown of CITED2 led to a decreased interaction of p53 with its inhibitor MDM2, which results in increased amounts of total p53 protein. Data indicate that CITED2 functions in pathways regulating p53 activity. PMID: 29072699
    37. his study demonstrates that inhibition of Drp1 hyperactivation by a Drp1 peptide inhibitor P110 is neuroprotective in a MPTP animal model. Our data also suggest that the protective effects of P110 treatment might be mediated by inhibiting the p53 mediated apoptotic pathways in neurons through inhibition of Drp1-dependent p53 mitochondrial translocation PMID: 27619562
    38. these results indicate that IGF-I induces senescence of hepatic stellate cells, inactivates these cells and limits fibrosis in a p53-dependent manner and that IGF-I may be applied to treat nonalcoholic steatohepatitis and cirrhosis. PMID: 27721459
    39. Conditional deletion of uterine Trp53 (p53(d/d)), molecularly linked to mTORC1 activation and causally linked to premature uterine senescence and preterm birth, results in aberrant lipid signatures within the heterogeneous cell types of embryo implantation sites on day 8 of pregnancy. PMID: 27620843
    40. These results suggest that ASPP2 plays an important role in p53-mediated neuronal apoptosis under gp120 stress. PMID: 27625111
    41. This study shows that when cell death and cell cycle arrest pathways are inhibited, p53 can still mediate MGMT-dependent repair, to promote cell survival upon DNA damage. PMID: 28753207
    42. DNA repair, mediated by DNA-PK, and cell cycle arrest, mediated by p53, cooperate to protect the stem cell niche after DNA damage. PMID: 28686579
    43. Arf (smArf) protein corrects p53-independent developmental defects of Arf tumor suppressor-deficient mice PMID: 28652370
    44. in the absence of the tumor suppressor p53, germline genetic deletion of AMPK beta1 accelerates the appearance of a T-cell lymphoma that reduces lifespan compared to p53 deficiency alone. PMID: 28544264
    45. The results suggested that TAZ may suppress apoptosis and premature senescence in spermatogenic cells by inhibiting the p53-p21 signaling pathway, thus playing important roles in the maintenance and control of reproductive function. PMID: 28613007
    46. These results illustrate the importance of the cooperative activities of p53 and Mdm2 in a network of miRNAs that function to impose a barrier against aberrant cardiomyocyte cell cycle re-entry to maintain cardiac homeostasis. PMID: 28745540
    47. these observations suggest that hypoxic preconditioning of cardiac progenitor cells could lead to p53 inhibition by up-regulating PMID: 27488808
    48. These data indicate the functional role of the MIF-COX-p53 axis in inflammation and cancer at the genomic and proteomic levels in COX-2-ablated cells. PMID: 29247872
    49. p53 limits ferroptosis of colorectal cancer cells by blocking DPP4 activity. PMID: 28813679
    50. FGF1 protects neuroblastoma cells from p53-dependent apoptosis through an intracrine pathway regulated by FGF1 phosphorylation. PMID: 29048426

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  • 相关疾病：
    p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    P53 family
  • 数据库链接：

    KEGG: mmu:22059

    STRING: 10090.ENSMUSP00000104298

    UniGene: Mm.222