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  4. Recombinant Mouse Cell division control protein 42 homolog (Cdc42)

Recombinant Mouse Cell division control protein 42 homolog (Cdc42)

  • 货号：
    CSB-YP005008MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005008MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005008MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005008MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005008MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc42Cell division control protein 42 homolog; EC 3.6.5.2; G25K GTP-binding protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-188
  • 氨基酸序列
    MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP EPKKSRRC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In its active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
  • 基因功能参考文献：
    1. We concluded that CDC42 negatively regulates SEPT12 polymerization and is involved in terminal structure formation of sperm heads. PMID: 30189608
    2. These results of this study suggest that Cdc42 and Rac1 synergistically function in BG during cerebellar corticogenesis. PMID: 29253508
    3. Cdc42 deficiency impairs endothelial cell function and regeneration after inflammatory vascular injury. PMID: 29422044
    4. MiR-7 inhibited peripheral nerve injury repair by affecting neural stem cells migration and proliferation through cdc42. PMID: 29663842
    5. downregulation of Cdc42, but not Rac1, is responsible for the unusual biophysical features of tumor repopulating cells. PMID: 29673588
    6. Rac1 and Cdc42 have cooperating roles in regulation of bone development. PMID: 29626467
    7. results suggest epithelium cell-specific Cdc42 deletion leads to tooth hypomaturation and transformation of the enamel prism structure that is likely due to altered ameloblast morphology and the secretion of enamel matrix proteins and proteases. This is the first in vivo evidence suggesting that Cdc42 is essential for proper tooth development and amelogenesis. PMID: 29684584
    8. Although much of the basic actin machinery was intact, Cdc42 null cells lacked the ability to polarize their Golgi and coordinate motility systems for efficient movement. Loss of Cdc42 de-coupled three main systems: actin assembly via the formin FMNL2 and Arp2/3, active myosin-II localization, and integrin-based adhesion dynamics. PMID: 28238662
    9. Cdc42 is required for endothelial tip cell selection, directed cell migration and filopodia formation, but dispensable for cell proliferation or apoptosis. Although the loss of Cdc42 seems generally compatible with apical-basal polarization and lumen formation in retinal blood vessels PMID: 29853619
    10. Cdc42 bypasses the need for exogenous fibronectin by stimulating cellular fibronectin deposition under the newly formed lamellipodia. PMID: 28754687
    11. Simulated microgravity activates Cdc42 via Rap1GDS1 to promote vascular branch morphogenesis. PMID: 29145128
    12. In contrast to neuronal cells, Botulinum neurotoxin type B uses a Cdc42-dependent pathway to enter intestinal cells. PMID: 28296078
    13. We conclude that CRN7 spatiotemporally influences F-actin organization and Golgi integrity in a Cdc42- and N-WASP-dependent manner. PMID: 27143109
    14. the expression of CDC42 might be regulated by AHR, and both proteins are fundamental to the development of normal spermatozoa and the acrosome reaction. PMID: 27635527
    15. CDC42 loss suppresses acute myeloid leukemia cell polarity and division asymmetry. PMID: 28778865
    16. CDC42 is involved in the trafficking of FGF receptors to the cell membrane to regulate epicardium formation. PMID: 28465335
    17. LRCH1 as a novel effector to restrain PKCalpha-DOCK8-Cdc42 module-induced T cell migration and ameliorate experimental autoimmune encephalomyelitis (EAE). PMID: 28028151
    18. analysis of phenotypes of Cdc42 and Myo10 deletion that show multiple roles of filopodial dynamics PMID: 28289096
    19. Finally, we demonstrate that Cdc42 is involved in neuroligin-dependent presynaptic differentiation of proprioceptive sensory neurons in vitro These data suggest that Cdc42 in presynaptic sensory neurons is essential for proper synapse formation in the development of monosynaptic sensory-motor circuits. PMID: 27225763
    20. found that DOCK8 associated with LRAP35a, an adaptor molecule that binds to the Cdc42 effector myotonic dystrophy kinase-related Cdc42-binding kinase, and facilitated its activity to phosphorylate myosin II regulatory light chain PMID: 28028174
    21. These results indicate that HuR promotes early intestinal mucosal repair after injury by increasing Cdc42 translation. PMID: 28031329
    22. Lrrk1 deficiency in osteoclasts resulted in reduced phosphorylation and activation of RAC1/Cdc42 PMID: 27600824
    23. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway. PMID: 28389565
    24. Cdc42 is essential for cardiomyocyte proliferation, sarcomere organization and cell-cell adhesion during heart development. PMID: 27986432
    25. Cdc42 is involved not in osteogenesis but in chondrogenesis in which the BMP2/Cdc42/Pak/p38/Smad signaling module promotes mesenchymal condensation and the TGF-beta/Cdc42/Pak/Akt/Sox9 signaling module facilitates chondrogenic differentiation. PMID: 26739452
    26. These data show that membrane protrusions originate from surface ectodermal cells and that Rac1 is necessary for the formation of membrane ruffles which typify late neural fold closure stages, whereas Cdc42 is required for the predominance of filopodia in early neurulation. PMID: 27114066
    27. findings demonstrate that Cdc42 plays essential roles in mammary gland function post pregnancy, where it helps to establish proper epithelial cell polarity and tissue homeostasis during lactation. PMID: 26912661
    28. This work demonstrated miR-137 and CDC42 are critical regulators in cardiomyocyte apoptosis. PMID: 26566162
    29. the principal role of Cdc42 in ureteric bud and metanephric mesenchyme development is to regulate epithelial cell polarity and the actin cytoskeleton. PMID: 26490995
    30. indicate that restricted expression of Wnt5a in the caudal SpM is essential for normal OFT morphogenesis, and uncover a novel function of spatially regulated cell cohesion by Wnt5a in driving the deployment of SHF cells from the SpM into the OFT PMID: 26902112
    31. role of P-cadherin through beta-PIX-mediated Cdc42 activation in the regulation of cell polarity PMID: 26783302
    32. Thus, Cdc42 and Rac1 have nonredundant roles in controlling ALK-rearranged lymphoma survival and morphology but are redundant for lymphoma dissemination PMID: 26747246
    33. Knockdown of Fat1 in renal tubular cells decreases active RAC1 and CDC42. PMID: 26905694
    34. Our findings demonstrate the importance of Cdc42 for cartilage development during both embryonic and postnatal stages. PMID: 26820532
    35. Data indicate enantiomer selective interaction of R-naproxen and R-ketorolac with Rho family GTPases Rac1 and Cdc42. PMID: 26558612
    36. results indicate that there is a crucial role for 5-HT7R-Cdc42-mediated signaling in the regulation of dendritic cell morphology and motility, suggesting that 5-HT7R could be a new target for treatment of a variety of inflammatory and immune disorders. PMID: 26092936
    37. Axin promotes dendritic spine stabilization through Cdc42-dependent cytoskeletal reorganization. PMID: 26204446
    38. NRP1 enables the extracellular matrix (ECM)-induced activation of CDC42, a key regulator of filopodia formation. PMID: 26051942
    39. Cdc42 control of actin dynamics keeps DCs in an immature state. PMID: 26553928
    40. to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. PMID: 26416959
    41. This work supports the notion of Cdc42 as a central regulator of the cellular machinery in endothelial cells that drives blood vessel formation. PMID: 26253403
    42. Results provide novel evidence that low expression of SLIT2 correlates with poor prognosis and promotes metastasis in ESCC, which may be regulated by the Cdc42-mediated pathways. PMID: 25490006
    43. deletion of Cdc42 impaired LTP in the Schaffer collateral synapses and postsynaptic structural plasticity of dendritic spines in CA1 pyramidal neurons; loss of Cdc42 did not affect memory acquisition, but instead impaired remote memory recall PMID: 25006034
    44. Our results demonstrate a critical role for Cdc42 in the motility of mature B cells, their cognate interaction with T cells, and their differentiation into Ab-producing cells PMID: 25870239
    45. PAK4 phosphorylates Par6B at Ser143 blocking its interaction with Cdc42. PMID: 25662318
    46. Data indicate cell division cycle 42 protein (Cdc42) as key regulator of B cell physiology. PMID: 25547673
    47. CDC42 has a crucial role in senescence-associated inflammation and atherosclerosis. PMID: 25057989
    48. demonstrate that constitutively-active Cdc42 binding to gammaCOP induced the accumulation of epithelial growth factor receptor (EGFR) in the cells PMID: 25497016
    49. It was concluded that Cdc42 is essential for cartilage development during endochondral bone formation. PMID: 25343271
    50. findings demonstrate that dual lipidation of Cdc42-palm represents an important regulator of morphogenic signalling in hippocampal neurons. PMID: 24059268

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, dendrite.
  • 蛋白家族：
    Small GTPase superfamily, Rho family, CDC42 subfamily
  • 数据库链接：

    KEGG: mmu:12540

    STRING: 10090.ENSMUSP00000030417

    UniGene: Mm.1022