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  4. Recombinant Mouse Caspase-6 (Casp6)

Recombinant Mouse Caspase-6 (Casp6)

  • 货号：
    CSB-YP004551MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004551MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004551MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004551MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004551MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Casp6Caspase-6; CASP-6; EC 3.4.22.59; Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18; Caspase-6 subunit p11]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    6-162
  • 氨基酸序列
    GFYKS REVFDPAEQY KMDHKRRGVA LIFNHERFFW HLTLPERRGT NADRDNLTRR FSDLGFEVKC FNDLRAEELL LKIHEVSTSS HIDADCFICV FLSHGEGNHV YAYDAKIEIQ TLTGLFKGDK CQSLVGKPKI FIIQACRGSQ HDVPVVPLDM VD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. During apoptosis, localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. Cleaves phospholipid scramblase proteins XKR4 and XKR9. Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis. Regulates B-cell programs both during early development and after antigen stimulation. In addition, promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses. Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death.
  • 基因功能参考文献：
    1. Intact motor axons of Casp6 null mice had normal electrophysiological properties but, as tested serially during axonal degeneration (AxD), there was attenuated loss of excitability. Following transection, morphological features of AxD were evident in both wild type and Casp6-/- mice but the latter had evidence of slowed progression. PMID: 29229552
    2. Caspase-6 is posttranslationally palmitoylated by the palmitoyl acyltransferase HIP14 and that the palmitoylation of Caspase-6 inhibits its activation. PMID: 27911442
    3. The bactericidal activity of caspase-6-/- macrophages was impaired compared to wild type cells. Caspase-6-/- mice showed higher expression of the IL-1b gene, known to be detrimental in murine melioidosis. Expression of the IL-10 gene was also increased in caspase-6-/- mice as early as 6 hours after infection. Treatment with exogenous IL-10 rendered mice more susceptible against B. pseudomallei challenge PMID: 28686630
    4. caspase-6 could regulate breast cancer cell invasion by modulating MMP-2 and MMP-9 expression in 4T1 tumor-associated macrophages PMID: 27325699
    5. Casp6 is unlikely to be involved in colitis-associated tumors. PMID: 25470254
    6. p53 activity is an important upstream regulator of caspase-6 activity in muscle tissue. PMID: 24070868
    7. TNFalpha-induced RIP1-independent caspase-6 activation was involved in regulating the relationship between autophagy and necroptosis. PMID: 23941769
    8. CASP6 released from axonal terminals regulates microglial TNF-alpha secretion, synaptic plasticity, and inflammatory pain. PMID: 24531553
    9. Casp6-/- neurons are protected against excitotoxicity, nerve growth factor deprivation and myelin-induced axonal degeneration. Furthermore, Casp6-deficient mice show an age-dependent increase in cortical and striatal volume. PMID: 22262731
    10. This study demonistrated that elimination of caspase-6 protein and activity in the BACHD mouse model does not prevent the production of a 586 aa Htt proteolytic fragment in the brain. PMID: 22649225
    11. Neutrophil/macrophage contact activates CASP-6, producing interleukin-1 receptor-associated kinase-M (IRAK-M) cleavage and de-repression of alveolar macrophages; CASP-6 deletion protects mice from death caused by bacterial peritonitis. PMID: 21098228
    12. Lamin A/C and caspase-6 could be valuable tools in the knowledge of oocyte in vitro destiny PMID: 20130288
    13. p53 activation enhances XIAP inhibition-induced cell death by promoting mitochondrial release of second mitochondria-derived activator of caspases (SMAC) and by inducing the expression of caspase-6. PMID: 19897582
    14. CREB-binding proteins are specifically targeted by caspases and calpains at the onset of neuronal apoptosis PMID: 14657026
    15. caspase-6 and caspase-7 are activated by teratogens in early postimplantation mouse embryos PMID: 14686614
    16. Taken together, these results suggest that activation of caspases (6 and 7) and the subsequent cleavage of lamin A/C and PARP are involved in the morphological changes of apoptotic macrophages infected with Actinobacillus actinomycetemcomitans. PMID: 17893180
    17. Casp6 may regulate both B cell activation and differentiation PMID: 18981099
    18. results indicate that APP and DR6 are components of a neuronal self-destruction pathway, and suggest that an extracellular fragment of APP, acting via DR6 and caspase 6, contributes to Alzheimer's disease PMID: 19225519

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Highly expressed in lung, liver, kidney, testis, and heart. Lower levels in spleen, skeletal muscle and brain. Expressed in neurons.
  • 数据库链接：

    KEGG: mmu:12368

    STRING: 10090.ENSMUSP00000029626

    UniGene: Mm.281379