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Recombinant Mouse Caspase-14 (Casp14)

  • 中文名称：
    Recombinant Mouse Caspase-14(Casp14),Yeast
  • 货号：
    CSB-YP004546MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Caspase-14(Casp14),Yeast
  • 货号：
    CSB-EP004546MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Caspase-14(Casp14),Yeast
  • 货号：
    CSB-EP004546MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Caspase-14(Casp14),Yeast
  • 货号：
    CSB-BP004546MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Caspase-14(Casp14),Yeast
  • 货号：
    CSB-MP004546MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Casp14
  • Uniprot No.：
  • 别名：
    Casp14Caspase-14; CASP-14; EC 3.4.22.-; Mini-ICE; MICE) [Cleaved into: Caspase-14 subunit p17; mature form; Caspase-14 subunit p10; mature form; Caspase-14 subunit p20; intermediate form; Caspase-14 subunit p8; intermediate form]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    8-167
  • 氨基酸序列
    PQP LQEERYDMSG ARLALTLCVT KAREGSEVDM EALERMFRYL KFESTMKRDP TAQQFLEELD EFQQTIDNWE EPVSCAFVVL MAHGEEGLLK GEDEKMVRLE DLFEVLNNKN CKALRGKPKV YIIQACRGEH RDPGEELRGN EELGGDEELG GDEVAVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro is equally active on the synthetic caspase substrates WEHD-ACF and IETD-AFC. Involved in processing of prosaposin in the epidermis. May be involved in retinal pigment epithelium cell barrier function.
  • 基因功能参考文献：
    1. Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models. PMID: 24872419
    2. caspase-14 overexpression is not the cause of utricle formation PMID: 23800061
    3. Caspase-14-deficient mice are more prone to the development of parakeratosis. PMID: 23014340
    4. Mere impairment of filaggrin degradation by loss of caspase 14 does not influence the inflammatory threshold of atopic dermatitis. PMID: 22931922
    5. Caspase-14 is required for filaggrin degradation to natural moisturizing factors in the skin. PMID: 21654840
    6. Transcript profiling studies identified caspase-14 as a novel downstream target of Gata-3, in keeping with its roles in differentiation and tumorigenesis. PMID: 21930782
    7. We investigated whether cardiac autonomic control is impaired during sleep in ob/ob mice with morbid obesity caused by congenital leptin deficiency. PMID: 20337194
    8. processing of caspase 14 in epidermal differentiation PMID: 12200134
    9. SH2-B dramatically enhanced leptin-stimulated tyrosine phosphorylation of IRS1 and IRS2 in human and mouse cells (this is supposed to be a NEWENTRY for mice--SH2-B). PMID: 15316008
    10. Caspase-14, but not caspase-3 activation coincides temporally and spatially with embryonic KC differentiation, suggesting a role for caspase-14 in terminally differentiated KC. PMID: 16316411
    11. The skin of caspase-14-deficient mice was shiny and lichenified, indicating an altered stratum-corneum composition. It contained significantly more alveolar keratohyalin F-granules, the profilaggrin stores. PMID: 17515931
    12. permeability barrier requirements coordinately drive both the generation of the stratum corneum lipid-enriched extracellular matrix and the transformation of granular cells into corneocytes, in an SP- and Casp-14-dependent manner, signaled by PAR2 PMID: 18156206

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Embryo, adult liver and less in adult brain and kidney. Expressed in differentiating keratinocytes of embryonic skin (at protein level). Expressed in keratinocytes of adult skin suprabasal layers (at protein level).
  • 数据库链接：

    KEGG: mmu:12365

    STRING: 10090.ENSMUSP00000005488

    UniGene: Mm.20940