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  4. Recombinant Mouse Casein kinase II subunit alpha (Csnk2a1)

Recombinant Mouse Casein kinase II subunit alpha (Csnk2a1)

  • 货号：
    CSB-YP717130MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP717130MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP717130MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP717130MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP717130MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Csnk2a1; Ckiia; Casein kinase II subunit alpha; CK II alpha; EC 2.7.11.1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-391
  • 氨基酸序列
    MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMSSTSMA GGSTPVSSAN MMSGISSVPT PSPLGPLAGS PVIAAANSLG IPVPAAAGAQ Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, ATF4, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Mediates sequential phosphorylation of FNIP1, promoting its gradual interaction with Hsp90, leading to activate both kinase and non-kinase client proteins of Hsp90. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry.
  • 基因功能参考文献：
    1. these data suggest that Fcgamma receptor/reactive oxygen species/CK2alpha is a key inducer of NF-kappaB activation in airway epithelial cells in a murine model of asthma PMID: 29339659
    2. Data (including data from studies using knockout mice) suggest that Brg1 is phosphorylated by casein kinase 2 (Ck2; Ck2alpha1 and Ck2alpha-prime are catalytic subunits) in proliferating skeletal myoblasts; Brg1 is catalytic subunit of SWI/SNF chromatin-remodeling enzymes; Ck2-mediated phosphorylation of Brg1 appears to regulate myoblast proliferation. (Brg1 = Brahma-related gene 1 protein) PMID: 28939766
    3. Casein kinase 2 plays a central role in striatal physiology and in severity of l-DOPA-induced dyskinesia. PMID: 29097596
    4. Casein kinase 1 (alpha, delta and epsilon) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression. PMID: 25927854
    5. These data support a model in which activated Akt enhances rRNA synthesis both by preventing TIF-IA degradation and phosphorylating CK2alpha, which in turn phosphorylates TIF-IA. PMID: 24297901
    6. An essential role of CK2alpha in the mammalian circadian clock. PMID: 23840637
    7. receptor beta and its domains interact with casein kinase 2, phosphokinase C, and N-myristoylation sites of mitochondrial and nuclear proteins in mouse brain PMID: 22566700
    8. CK2alpha levels are predominant in comparison to CK2alpha' levels throughout the mammalian brain. PMID: 21761202
    9. The overall phenotype found in CK2alpha (-/- ) mice, is described. PMID: 21761203
    10. These experiments demonstrate that combined loss of one CK2alpha allele and both CK2alpha' alleles leads to unique abnormalities of growth and development. PMID: 21769451
    11. TNP1 is a newly identified binding partner of the alpha-subunit of casein kinase 2. PMID: 20524034
    12. Casein kinase II has a role in phosphorylation of NF-kappaB p65 subunit enhancement of inducible nitric-oxide synthase gene transcription PMID: 15033982
    13. CK2 selectively phosphorylates MKP3, suggesting cross-regulation between CK2alpha and MKP3, as well as a modulation of ERK2-MAPK signaling by CK2alpha via MKP3 PMID: 15284227
    14. In cultured hippocampal neurons, the capacity of Nerve Growth Factor to promote axon elongation is mostly mediated by p75NTR, and the activation of this pathway leads to the inactivation of GSK-3beta. PMID: 16723502
    15. CK2 involvement in the phenomenon of the drug resistance was investigated. PMID: 18560764
    16. CK2alpha has an essential role in embryogenesis, as mice lacking CK2alpha die in mid-embryogenesis, with cardiac and neural tube defects. PMID: 18594950
    17. These results suggest that Ikaros acts as a negative regulator on iNOS expression. PMID: 18818879
    18. The balance between beta-arr and CK1/2 determines whether WNT/RAC-1 or other non-canonical WNT pathways are activated. PMID: 18953287
    19. reversible phosphorylation of CK2alpha requires precise regulation to allow proper mitotic progression PMID: 19188443
    20. CAPS activity in priming vesicle exocytosis requires CK2 phosphorylation PMID: 19460754

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
  • 数据库链接：

    KEGG: mmu:12995

    STRING: 10090.ENSMUSP00000096829

    UniGene: Mm.23692