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  4. Recombinant Mouse Casein kinase I isoform epsilon (Csnk1e)

Recombinant Mouse Casein kinase I isoform epsilon (Csnk1e)

  • 中文名称：
    Recombinant Mouse Casein kinase I isoform epsilon(Csnk1e),Yeast
  • 货号：
    CSB-YP878118MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Casein kinase I isoform epsilon(Csnk1e),Yeast
  • 货号：
    CSB-EP878118MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Casein kinase I isoform epsilon(Csnk1e),Yeast
  • 货号：
    CSB-EP878118MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Casein kinase I isoform epsilon(Csnk1e),Yeast
  • 货号：
    CSB-BP878118MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Casein kinase I isoform epsilon(Csnk1e),Yeast
  • 货号：
    CSB-MP878118MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Csnk1e
  • Uniprot No.：
  • 别名：
    Csnk1e; Casein kinase I isoform epsilon; CKI-epsilon; CKIe; EC 2.7.11.1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-416
  • 氨基酸序列
    MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH IESKFYKMMQ GGVGIPSIKW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFST YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGAARN PEDVDRERRE HEREERMGQL RGSATRALPP GPPTGATANR LRSAAEPVAS TPASRIQQTG NTSPRAISRA DRERKVSMRL HRGAPANVSS SDLTGRQEVS RLAASQTSVP FDHLGK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
  • 基因功能参考文献：
    1. CK1delta/epsilon disruption mouse had a long (27.4 h) behavioral period to which peripheral clocks entrained in vivo, albeit with an advanced phase ( approximately 6 h). PMID: 29463694
    2. Studied roles of CK1delta and CK1e in regulation of PER2 in circadian rhythm. PMID: 29784789
    3. the impact of obesity on the circadian system in mice at a behavioural and molecular level was assessed, and determined whether pharmacological targeting of casein kinase 1delta and epsilon (CK1delta/epsilon), key regulators of the circadian clock, can confer metabolic benefit. PMID: 27439882
    4. Csnk1e deletion increased mu opioid receptor-dependent behaviors. PMID: 28594147
    5. Here, the s identify another Wnt signaling amplifier, CKIepsilon, which is specifically upregulated in intestinal stem cells and is essential for intestinal stem cell maintenance, especially in the absence of its close isoform CKIdelta. PMID: 28963394
    6. Linkage mapping, transcript covariance and pharmacological testing suggest that genetic variation affecting Csnk1e may contribute to individual differences in von Frey filament response. PMID: 27231153
    7. mice bearing a short-period mutation in the enzyme casein kinase 1epsilon (tau mutation), which accelerates free-running circadian cycles, were investigated. PMID: 26715747
    8. Data indicate period circadian proteins PER1 and PER2 as direct substrates of casein kinase 1epsilon (CK1epsilon). PMID: 24682205
    9. Integrated genetic approaches identify the molecular mechanisms of Sox4 in early B-cell development: intricate roles for RAG1/2 and CK1epsilon. PMID: 24786772
    10. CK1 epsilon(tau), as a gain-of-function mutation, acts at a specific circadian phase to promote degradation of PERIOD proteins and thereby accelerate the mammalian clockwork in brain and periphery. PMID: 18400165
    11. A narrow genetic locus containing Csnk1e is associated with differences in sensitivity to methamphetamine (MA) and fentanyl; variation at the Csnk1e locus can regulate the locomotor response to MA. PMID: 22089318
    12. The results suggest that CK1delta/epsilon binds to and phosphorylates the N-terminal domain of Dnmt1 and regulates Dnmt1 function by reducing the DNA-binding activity. PMID: 20192920
    13. physical interaction between PER and CKIdelta/epsilon required for circadian rhythm PMID: 19948962
    14. Cell/organ type-specific function of CK1epsilon indicate that tumorigenic conversion of mammary glands in SV40 T-antigen-transgenic mice leads to downregulation of CK1epsilon. PMID: 19755715
    15. Results suggest that the metabotropic glutamate receptor agonist DHPG activates casein kinase I (CK1) epsilon via Ca(2+)-dependent stimulation of calcineurin and subsequent dephosphorylation of inhibitory C-terminal autophosphorylation sites. PMID: 12223474
    16. the inability of PER3 to support circadian clock function results in part from lack of direct and stable interaction with casein kinase Iepsilon (CKIepsilon) PMID: 14701732
    17. casein kinase I epsilon activity is regulated by Wnt signaling PMID: 14722104
    18. Results suggest that casein kinase Iepsilon functions as a molecular switch to direct Dishevelled (Dvl) from the c-Jun N-terminal kinase pathway to the beta-catenin pathway, possibly by altering the conformation of the C terminus of Dvl. PMID: 14966280
    19. CK1 regulates fast synaptic transmission by glutamate in brain. Activation of CK1 decreases NMDA receptor activity in the striatum via activation by this kinase of protein phosphatase 1 &/or 2A & increased dephosphorylation of NMDA receptors. PMID: 16014721
    20. Transmissible colonic hyperplasia was associated with increases in casein kinase I-epsilon cellular abundance. PMID: 17053159
    21. Results suggest that GSK3beta and CK1/2 are the major protein kinases that contribute to removal of NFATc1 that accumulates in muscle fibre nuclei during muscle activity. PMID: 17185343
    22. CK1 has a role in preventing Abeta formation in Alzheimer's disease PMID: 17360493
    23. The degradation rate of PER2, which is regulated by CKIepsilon/delta-dependent phosphorylation, was temperature-insensitive in living clock cells. PMID: 19805222

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
  • 组织特异性：
    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • 数据库链接：

    KEGG: mmu:27373

    STRING: 10090.ENSMUSP00000113341

    UniGene: Mm.30199