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  4. Recombinant Mouse Casein kinase I isoform delta (Csnk1d)

Recombinant Mouse Casein kinase I isoform delta (Csnk1d)

  • 货号：
    CSB-YP884068MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP884068MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP884068MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP884068MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP884068MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Csnk1d; Hckid; Casein kinase I isoform delta; CKI-delta; CKId; EC 2.7.11.1; Tau-protein kinase CSNK1D; EC 2.7.11.26
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-415
  • 氨基酸序列
    MELRVGNRYR LGRKIGSGSF GDIYLGTDIA AGEEVAIKLE CVKTKHPQLH IESKIYKMMQ GGVGIPTIRW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFAT YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGASRA ADDAERERRD REERLRHSRN PATRGLPSTA SGRLRGTQEV APPTPLTPTS HTANTSPRPV SGMERERKVS MRLHRGAPVN VSSSDLTGRQ DTSRMSTSQI PGRVASSGLQ SVVHR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate.
  • 基因功能参考文献：
    1. CK1delta/epsilon disruption mouse had a long (27.4 h) behavioral period to which peripheral clocks entrained in vivo, albeit with an advanced phase ( approximately 6 h). PMID: 29463694
    2. Studied roles of CK1delta and CK1e in regulation of PER2 in circadian rhythm. PMID: 29784789
    3. the impact of obesity on the circadian system in mice at a behavioural and molecular level was assessed, and determined whether pharmacological targeting of casein kinase 1delta and epsilon (CK1delta/epsilon), key regulators of the circadian clock, can confer metabolic benefit. PMID: 27439882
    4. Here, the s identify another Wnt signaling amplifier, CKIepsilon, which is specifically upregulated in intestinal stem cells and is essential for intestinal stem cell maintenance, especially in the absence of its close isoform CKIdelta. PMID: 28963394
    5. Report temperature-compensated CKIdelta-dependent multi-site phosphorylation in mammals underlies temperature compensated phosphorylation in circadian clocks. PMID: 28886336
    6. Together, these findings suggest that CK1delta contributes to the efficient repair of DNA damage and the proper functioning of mitotic checkpoints by maintaining appropriate levels of Chk1. PMID: 28125685
    7. we conclude that APC/C(Cdh1) controls CK1 delta levels to balance proliferation and cell-cycle exit in the developing CNS PMID: 25843713
    8. CK1delta mediates primary ciliogenesis by multiple mechanisms, one involving its centrosomal function and another dependent on its interaction with AKAP450 at the Golgi. PMID: 24648492
    9. this article reports the crystallization and structure determination of a new crystal form of casein kinase I delta belonging to space group P21. PMID: 24100552
    10. decreases in CKIdelta activity can contribute to the pathogenesis of migraine. PMID: 23636092
    11. REGgamma-deficient mice develop premature aging phenotypes that are associated with abnormal accumulation of casein kinase (CK)1delta and p53. Antibody array analysis identified CK1delta as a direct target of REGgamma. PMID: 23766372
    12. inactivating mutations in CK1delta impair SV40-induced cellular transformation in vitro and mouse mammary carcinogenesis in vivo PMID: 22235331
    13. CK1delta plays a more prominent role than CK1epsilon in the maintenance of 24-hour rhythms in the master circadian oscillator PMID: 20421981
    14. These results provide strong evidence that the centrosomal localization of CK1delta is required for Wnt-3a-dependent neuritogenesis. PMID: 21422228
    15. The results suggest that CK1delta/epsilon binds to and phosphorylates the N-terminal domain of Dnmt1 and regulates Dnmt1 function by reducing the DNA-binding activity. PMID: 20192920
    16. physical interaction between PER and CKIdelta/epsilon required for circadian rhythm PMID: 19948962
    17. Results indicate that CKIdelta plays several roles in lymphocyte physiology. PMID: 12924632
    18. snapin is a new substrate of CK1delta PMID: 17101137
    19. The balance between beta-arr and CK1/2 determines whether WNT/RAC-1 or other non-canonical WNT pathways are activated. PMID: 18953287
    20. generated mice with liver-specific disruption of CK1delta. In livers from these mice, daytime levels of nuclear PER proteins, and PER-CRY-CLOCK complexes were elevated. In vitro, the half-life of PER2 was increased by approximately 20%. PMID: 19414593
    21. The degradation rate of PER2, which is regulated by CKIepsilon/delta-dependent phosphorylation, was temperature-insensitive in living clock cells. PMID: 19805222

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton, spindle. Golgi apparatus.
  • 蛋白家族：
    Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
  • 组织特异性：
    Expressed ubiquitously. However, kinase activity is not uniform, with highest kinase activity in splenocytes.
  • 数据库链接：

    KEGG: mmu:104318

    STRING: 10090.ENSMUSP00000018274

    UniGene: Mm.216227