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  4. Recombinant Mouse Calpain-8 (Capn8)

Recombinant Mouse Calpain-8 (Capn8)

  • 货号：
    CSB-YP849644MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP849644MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP849644MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP849644MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP849644MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Capn8
  • Uniprot No.：
  • 别名：
    Capn8; Ncl2Calpain-8; EC 3.4.22.53; New calpain 2; nCL-2; Stomach-specific M-type calpain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-703
  • 氨基酸序列
    MAALAAGISK QRAAAQGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE FPACPSALGY RDLGPGSAET QGIIWKRPTE LCSNPQFIVG GATRTDIRQG GLGDCWLLAA IASLTLNEKL LYRVVPRDQS FQKNYAGIFH FQFWQYGEWV EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL LEKAYAKLNG SYEALAGGST IEGFEDFTGG ISEFYDLRKP PGNLYYTIQK ALRKGSLLGC SIDVSNAAEA EATTRQKLVK GHAYSVTGVE EVDFRGLPEK LIRLRNPWGE VEWTGAWSDS APEWNYIDPQ KKGELDKRAE DGEFWMSFSD FLKQFSRLEI CNLSPDSLSS EEIHKWNLVL FNGRWTRGST AGGCQNYPAT YWTNPQFKIH LDEVDEDQEE GTSEPCCTVL LGLMQKNRRR QRRIGQGMLS IGYAVYQIPK ELENHTDEHL GRDFFQGRQP STCSSTYMNL REVSSRVQLP PGQYLVVPST FEPFKDGDFC LRVFSEKKAQ ALEIGDAVPG DPHEPHPRDM DGEDEHFWSL SEEFADKDSE ISAHQLKRVL NGLLSKRTDM KFDGFNINTC REMISLLDGD GTGSLRPVEF KTLWLKICKY LEIYQEMDHS RAGTIDAHEM RTALKKAGFT LNNQVQQTIA TRYACSKLGV DFDGFVACMI RLEILFKLFR LLDKDQNGIV QLSLAEWLCR ALV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex.
  • 基因功能参考文献：
    1. gastrointestinal-tract-specific calpains 8 and 9 formed a complex we termed "G-calpain," in which both were essential for gastric mucosal defense PMID: 20686710
    2. nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP PMID: 16476741
    3. nCL-2/calpain 8 is active without calpain regulatory subunit and oligomerizes through C2-like domains PMID: 17646163
  • 亚细胞定位：
    Cytoplasm. Golgi apparatus.
  • 蛋白家族：
    Peptidase C2 family
  • 组织特异性：
    Predominantly expressed in the stomach. Localizes strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum.
  • 数据库链接：

    KEGG: mmu:170725

    STRING: 10090.ENSMUSP00000047164

    UniGene: Mm.189201