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  4. Recombinant Mouse Calpain-1 catalytic subunit (Capn1)

Recombinant Mouse Calpain-1 catalytic subunit (Capn1)

In Stock
  • 货号：
    CSB-EP004490MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Capn1; Canp1; Capa1Calpain-1 catalytic subunit; EC 3.4.22.52; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Micromolar-calpain; muCANP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    86.1kDa
  • 表达区域：
    1-713aa
  • 氨基酸序列
    MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVTFDLFKWLQLTMFA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-388' and 'His-410'.
  • 基因功能参考文献：
    1. Calpain-1 deficiency impairs mGluR-dependent LTD and fear memory extinction. PMID: 28202907
    2. calpain contributes to the regulation of new gene expression that is necessary for these memory processes as a regulator of Ca(2+)-signal transduction pathway. PMID: 29258546
    3. the present study suggested that ephrinB/EphB signaling contributes to spinal nociceptive processing via the actions of calpain1 and caspase3. PMID: 29749521
    4. s provide the first evidence that calpain-1 regulates platelet hyperactivity in sickle mice. PMID: 29101791
    5. A total of 55 genes were differentially expressed in micro-calpain knockout mice. PMID: 29775750
    6. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
    7. indicate that calpain-1 and calpain-2 play opposite roles in high increased intraocular pressure-induced ischemic injury PMID: 27185592
    8. Results strengthen the roles of both calpain-1 and PHLPP1 in synaptic plasticity and learning and memory, and illustrate the complexities of the interactions between multiple pathways participating in synaptic plasticity. PMID: 27421891
    9. Genetic ablation of calpain-1 ameliorates chronic pain behavior in sickle cell mice. PMID: 27418647
    10. Our results indicate that calpain mediates cytokine-induced collagen-I synthesis and proliferation of ASMCs via the mTORC2/Akt signalling pathway, thereby regulating airway smooth muscle remodelling in asthma. PMID: 27649066
    11. mutations in CAPN1 are an additional cause of ataxia in mammals, including humans. PMID: 27320912
    12. Results indicate that calpain-1 and calpain-2 are differentially activated in hippocampus following seizure activity, with calpain-1 activation being limited to a small population of interneurons, and possibly triggering neuroprotective events, while calpain-2 is widely activated in pyramidal neurons of CA1 and CA3, and triggers neurodegenerative cascades. PMID: 27622212
    13. Here we show the combined loss of glial glutamate transporters GLT1 and GLAST in spinal cord caused motor neuronal death and hindlimb paralysis. PMID: 28821644
    14. These results suggested that Streptococcus pneuomoniae PLY induces the influx of calcium in Streptococcus pneumoniae-infected macrophages, followed by calpain activation and subsequent IL-1alpha maturation and secretion. PMID: 28630064
    15. High calpain expression is associated with brain injury. PMID: 27875293
    16. the expression of the full-length 60-kDa calcineurin protein is down-regulated due to over-activated calpain that cleaves calcineurin to form a 45-kDa fragment. PMID: 27171192
    17. calpains and calpastatin in patients with idiopathic pulmonary arterial hypertension (PAH) and mice with hypoxic or spontaneous (SM22-5HTT(+) strain) PH, were investigated. PMID: 26974350
    18. Attenuation of atherosclerotic lesions/inflammation in ApoE KO mice by simvastatin might be associated with the downregulation of CD36 and calpain-1. PMID: 28448973
    19. Leukocyte calpain-1/calpain-2 deficiency reduces angiotensin II-induced inflammation and atherosclerosis but not abdominal Aortic aneurysms in mice. PMID: 26966280
    20. Taken together, our findings suggested that DHRS7C maintains intracellular Ca(2+) homeostasis involving the ER/SR and that functional loss of DHRS7C leads to Ca(2+) overload in the cytosol and ER/SR, resulting in enlarged cellular morphology via calpain activation PMID: 27806939
    21. In conclusion, calpain was involved in oxygen glucose deprivation-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF. PMID: 27752886
    22. CRYbetaA3/A1-crystallin has a role in preventing nuclear cataract impaired lysosomal cargo clearance and calpain activation PMID: 26863613
    23. Capn1 protein and activity in mitochondria were elevated in diabetic mouse hearts. Increased mitochondrial Capn1 was associated with increased mitochondrial ROS generation and oxidative damage and reduced ATP5A1 protein and ATP synthase activity. PMID: 26470784
    24. LOX-1 is, at least in part, responsible for the inhibitory effect of ox-LDL on macrophage migration and this process involves calpain-1 and -2. PMID: 26393906
    25. Over-expression of Cast inhibits calpain activation and attenuates post-infarction myocardial remodeling. PMID: 25786109
    26. Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. PMID: 26063807
    27. Data indicate that calpain-1 up-regulation induces endoplasmic reticulum (ER) stress. PMID: 25660447
    28. Calcitriol stimulates expression of mu-calpain in co-cultured mouse cells. PMID: 24687633
    29. Data suggest that vitamin D3 up-regulates skeletal muscle fat deposition, up-regulates muscle u-calpain/Capn1 and m-calpain/Capn2 expression, and down-regulates muscle calpastatin expression. PMID: 25520983
    30. Calpain-mediated MFN2 degradation is a novel mechanism regulating mitochondrial fusion during glutamate excitotoxicity. PMID: 25416777
    31. in spinal cord of 30 day old mice, activation of calpain-1 produces high amounts of nNOS active species, while in 120 day old mice enhanced-prolonged activation of calpain-1 inactivates nNOS and down-regulates NR2B PMID: 25151305
    32. Overexpression of the calpain-specific inhibitor calpastatin reduces human alpha-synuclein processing, aggregation and synaptic impairment in transgenic mice. PMID: 24619358
    33. Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal aortic aneurysms in calpain-1 deficient mice. PMID: 23977256
    34. Our findings support the therapeutic promise of highly specific calpain inhibition in the treatment of tauopathies and other neurodegenerative states. PMID: 25009256
    35. The suppressive effects of PDGF-BB on Ca(2+) overload in neurons were more potent than those of PDGF-AA. PMID: 24454980
    36. calpain-1 is identified as a novel regulator in IgE-mediated mast cell activation and could serve as a potential therapeutic target for the management of allergic inflammation. PMID: 24760147
    37. The results of the present study highlight a dual role for calpain-1 in the weaned gland after the pregnancy/lactation cycle, controlling programmed cell death and participating in the epigenetic programme during adipocyte differentiation. PMID: 24467364
    38. Calpain induced myocardial NF-kappaB activation, TNF-alpha expression, and myocardial dysfunction in septic mice through IkappaBalpha protein cleavage. PMID: 24441549
    39. These data contribute to the existing evidence of the importance of the calpain system's involvement in muscle growth, development, and atrophy. PMID: 23798514
    40. synaptic NMDAR-coupled mu-calpain activation is neuroprotective PMID: 24285894
    41. The calpain/calpastatin system has opposing roles in growth and metastatic dissemination of melanoma. PMID: 23565252
    42. data suggest that skNAC controls myoblast migration and sarcomere architecture in a calpain-dependent manner PMID: 23662692
    43. Activated skeletal muscle calpain regulates acetylcholine release in motor nerve terminals via nitric oxide and neural microRNAs, a novel mechanism for the modulation of synaptic activity in normal or pathological conditions. PMID: 23616539
    44. Both CAPN-1 and -2 were induced after weaning and its activity increased in isolated mitochondria and lysosomes. PMID: 22555453
    45. This study demonistrated that conditional model of calpain deficiency in the CNS has provided a clearer understanding of calpain biology in the brain regarding CNS development, synaptic plasticity, and specific neuronal death paradigms. PMID: 23536090
    46. Calpain-1 gene deletion improved RBC deformability without any effect on RBC lifespan. Ankyrin, band 3, protein 4.1R, adducin & dematin were not degraded. K+-Cl- co-transporter & the Gardos channel were reduced. PMID: 22870887
    47. Potentiation of the platelet spreading phenotype in calpain-1(-/-) mice suggests a novel role of calpain-1 in hemostasis. PMID: 22458296
    48. Increased STEP and calpain activation contribute to altered NMDAR localization in an Huntington's disease mouse model. PMID: 22523092
    49. Increased lipid peroxidation and low-density lipoprotein oxidation in the brain of hyperlipidemic mice are associated with increased activation of calpain-1/2. PMID: 21836084
    50. Calpain cleaves and activates the TRPC5 channel to participate in semaphorin 3A-induced neuronal growth cone collapse. PMID: 22547824

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  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Peptidase C2 family
  • 数据库链接：

    KEGG: mmu:12333

    STRING: 10090.ENSMUSP00000025891

    UniGene: Mm.6221