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  4. Recombinant Mouse Calcyclin-binding protein (Cacybp)

Recombinant Mouse Calcyclin-binding protein (Cacybp)

  • 货号：
    CSB-YP875155MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP875155MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP875155MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP875155MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP875155MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cacybp; SipCalcyclin-binding protein; CacyBP; Siah-interacting protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-229
  • 氨基酸序列
    ASVLEELQK DLEEVKVLLE KSTRKRLRDT LTSEKSKIET ELKNKMQQKS QKKPELDNEK PAAVVAPLTT GYTVKISNYG WDQSDKFVKI YITLTGVHQV PTENVQVHFT ERSFDLLVKN LNGKNYSMIV NNLLKPISVE SSSKKVKTDT VIILCRKKAE NTRWDYLTQV EKECKEKEKP SYDTEADPSE GLMNVLKKIY EDGDDDMKRT INKAWVESRE KQAREDTEF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
  • 基因功能参考文献：
    1. involvement of CacyBP/SIP in the regulation of p38 kinase activity, in addition to that of ERK1/2, might point to the function of CacyBP/SIP in pro-survival and pro-apoptotic pathways. PMID: 28283909
    2. In undifferentiated and differentiated tumor cells, CacyBP level has an effect on the ERK1/2-CREB-BDNF pathway. PMID: 27180052
    3. Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP) was initially described as a binding partner of S100A6 in the Ehrlich ascites tumor cells and later as a Siah-1-interacting protein. Its role has been studied in various mouse tumors and cell lines. Review. PMID: 26873490
    4. sumoylated CacyBP/SIP is present in the cytoplasmic and not in the nuclear fraction. We have also established that lysine 16 is the residue which undergoes sumoylation in the CacyBP/SIP protein. PMID: 24078263
    5. different activity of CacyBP/SIP in neuroblastoma NB2a and colon cancer HCT116 cells might affect the ERK1/2 pathway in the differentiation or proliferation processes PMID: 22480271
    6. new insight into the interaction between S100 proteins and CacyBP/SIP PMID: 22295074
    7. SIP (-/-) embryonic fibroblasts have increased levels of cytosolic p27 and exhibit increased cell motility compared to wild-type cells. PMID: 21734459
    8. CacyBP/SIP exhibits a phosphatase activity toward ERK1/2 kinases while its E217K mutant does not. PMID: 21110948
    9. Cacybp is associated with acute lung injury PMID: 21297076
    10. Data indicated that CacyBP/SIP could simultaneously interact with tubulin and actin, suggesting that CacyBP/SIP might link actin and tubulin cytoskeletons. PMID: 20637809
    11. binds EF-hand proteins of the S100 family [CacyBP/SIP] PMID: 12042313
    12. Structural details of multiple sites of protein-protein interactions on SIP provide insight into the mechanism that drives the formation of the Siah-1 E3 ubiquitin ligase complex [SIP] PMID: 15996101
    13. SIP (CacyBP)-thymocytes have an impaired pre-TCR checkpoint with failure of TCRbeta gene rearrangement and increased apoptosis, resulting in reduced cellularity of the thymus. PMID: 16413921
    14. The results show the changing localization and levels of beta-catenin in the mouse uterus during decidualization and suggest potential roles for the BTRC and CACYBP E3 ligase mechanisms of beta-catenin ubiquitination in the uterus during decidualization. PMID: 17533218
    15. These results suggest that CacyBP/SIP, via its interaction with tubulin, might contribute to the differentiation of neuroblastoma NB2a cells. PMID: 17916393
    16. These data suggest that the binding of CacyBP/SIP with ERK1/2 might regulate Elk-1 phosphorylation/transcriptional activity and that S100A6 might further modulate this effect via Ca(2+)-dependent interaction with CacyBP/SIP and competition with ERK1/2. PMID: 19166809

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Cytoplasmic in unstimulated cultured neurons. Upon increase of calcium, it localizes to a ring around the nucleus. In neuroblastoma cells, after a Retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear and perinuclear fractions may be phosphorylated.
  • 组织特异性：
    Highly expressed in brain and EAT cells. Expressed at low level in heart, muscle, and at very low level in the liver, spleen, lung, kidney and stomach.
  • 数据库链接：

    KEGG: mmu:12301

    STRING: 10090.ENSMUSP00000014370

    UniGene: Mm.10702