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  4. Recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit delta (Camk2d)

Recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit delta (Camk2d)

  • 货号：
    CSB-YP761354MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP761354MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP761354MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP761354MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP761354MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Camk2d; Kiaa4163; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta; EC 2.7.11.17
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-499
  • 氨基酸序列
    ASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL KGAILTTMLA TRNFSAAKSL LKKPDGVKES TESSNTTIED EDVKARKQEI IKVTEQLIEA INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE NALSKSNKPI HTIILNPHVH LVGDDAACIA YIRLTQYMDG SGMPKTMQSE ETRVWHRRDG KWQNVHFHRS GSPTVPIKPP CIPNGKENFS GGTSLWQNI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.
  • 基因功能参考文献：
    1. surgical-induced Osteoarthritis mice treated with a miR-146a inhibitor significantly alleviated the destruction of articular cartilage via targeting Camk2d and Ppp3r2. PMID: 28383548
    2. we demonstrate that the deltaC isoform of CaMKII contributes significantly to myocardial damage following ex vivo I/R. Signaling occurs through NF-kappaB and TNF-alpha and acute inhibition of the generation or function of thesemolecules has a very robust protective effect inWT animals and in those expressing CaMKIIdeltaC. PMID: 28077321
    3. Phosphorylating Titin's Cardiac N2B Element by ERK2 or CaMKIIdelta Lowers the Single Molecule and Cardiac Muscle Force PMID: 26682816
    4. CaMK2d-dependent phosphorylation of RyR2-S2814 is significant in cardiomyopathy, independent of hypertrophy, induced by prolonged beta-adrenergic receptor stimulation. PMID: 26080362
    5. Data indicate that combined cardiomyocyte-specific deletion of CaM Kinase II CaMKIIdelta/gamma does not affect ischemia/reperfusion (I/R) injury. PMID: 25193973
    6. CaMKIIdelta activation and phosphorylation of downstream targets was enhanced in aromatase knockout hearts subjected to ischemia-reperfusion PMID: 25625588
    7. Oxidative stress was elevated in mitochondria from Gq versus wild-type mice and respiratory rates were lower; these changes in mitochondrial function were restored by CaMKIIdelta deletion. PMID: 25605649
    8. Cardiac CaM Kinase II genes delta and gamma contribute to adverse remodeling but redundantly inhibit calcineurin-induced myocardial hypertrophy. PMID: 25124496
    9. Reveal a novel in vivo function of CaMKIIdelta in regulating H3 phosphorylation and suggest a novel epigenetic mechanism by which CaMKIIdelta controls cardiac hypertrophy. PMID: 25421395
    10. CaMKIIdeltagamma couples noncanonical Wnt signaling to histone deacetylase 4 and myosin enhancer factor 2. PMID: 25489064
    11. developed an ionic model of the ventricular myocyte to interrogate potentially arrhythmogenic positive feedback in both control conditions and when CaMKIIdeltaC is overexpressed PMID: 24421356
    12. we show selective upregulation of CaMKIIdelta in adult cardiac fibroblasts following cardiac hypertrophy and assign a previously unrecognised role to CaMKII in regulating adult cardiac fibroblast function in normal and diseased mouse hearts. PMID: 23881186
    13. Camk2d expression and activity increase during arteriogenesis PMID: 23951185
    14. Our studies suggest that CaMKII is a molecular signal that couples increased reactive oxygen species with atrial fibrillation and that therapeutic strategies to decrease oxidized CaMKII may prevent or reduce it. PMID: 24030498
    15. CaMKII activation may not be required for abnormal impulse induction in stretched ventricular myocytes. PMID: 23279377
    16. can be phosphoryalated by CaMKIId, both in vitro and in vivo. PMID: 23220127
    17. Activation of a mitochondrial/ox-CaMKII pathway contributes to increased sudden death in diabetic patients after myocardial infarction. PMID: 23426181
    18. Phosphorylation at specific PEVK/titin N2B-unique sequence sites was decreased in CAMK2g/d double knockout mice. PMID: 23283722
    19. CaMKIIdelta exhibited consistent expression patterns between its mRNA and protein with both rising remarkably during osteoclastogenesis PMID: 22428553
    20. These results demonstrate that there are dramatic CaMKII-mediated effects on RyR Calcium release that occur via regulation of both RyR activation and termination processes. PMID: 22713561
    21. these results indicate that CaMKIIdelta2 downregulates insulin gene expression by Ser142 phosphorylation of CREB and reducing binding of CREB to CBP. PMID: 22554507
    22. Abnormality in beta-adrenergic-PKA signaling may contribute to cardiomyopathy and heart failure through dysregulation in the alternative splicing of CaMKIIdelta exons 14, 15, and 16 and up-regulation of CaMKIIdeltaC. PMID: 22132070
    23. Na(v)1.5-dependent increase in Na(+influx activates CaMKII, which in turn phosphorylates Na(v)1.5, further promoting Na(+) influx. Pharmacological inhibition of either CaMKII or Na(v)1.5 ameliorates cardiac dysfunction caused by excessive Na(+) influx. PMID: 21677263
    24. Data show that Pcp4 overexpression induces precocious neuronal differentiation, and is associated with an increase in CaMKIIdelta activation. PMID: 21491429
    25. In cardiomyocytes, [Ca2+]i-activated calmodulin kinase II (CaMKII) activates downstreamregulatory element (DRE) binding transcription factor DREAM, which consequently suppresses the expression of L-type calcium channel 1C-subunit gene (Cacna1c). PMID: 21486818
    26. overexpression induces diastolic dysfunction and arrhythmogenic triggers possibly via an enhanced late Na(+) current PMID: 21174213
    27. The multifunctional Ca2+/calmodulin-dependent kinase II delta (CaMKIIdelta) controls neointima formation after carotid ligation and vascular smooth muscle cell proliferation through cell cycle regulation by p21. PMID: 21193397
    28. CaMKIIdeltac overexpression in RyR2(R4496C+/-) knock-in mice increases the propensity toward triggered arrhythmias, which may impair survival. CaMKII contributes to further destabilization of a mutated RyR2 receptor. PMID: 21251589
    29. Camk2delta is poorly expressed in synaptic sites and may be critical for receptor recycling, by providing a mechanism by which postsynaptic acetylcholine receptor density is maintained at the neuromuscular junction in vivo. PMID: 20844140
    30. CaMKIIdelta plays a critical role in the development of heart failure in part by accumulation of p53 and induction of cardiomyocyte apoptosis in the dilated cardiomyopathy mouse model PMID: 20713897
    31. Mice engineered with an RyR2-S2814A mutation have RyR2 channels that cannot be phosphorylated by CaMKIIdelta, and exhibit a blunted positive force frequency relationship. PMID: 20479242
    32. This work has provided insight into the intracellular signaling pathways and transcription factors regulating Ncx1 gene expression in a chronically beta-AR-stimulated heart. PMID: 19945464
    33. Data indicate that cardiomyocytes from PLN-KO/CaMKII-TG mice showed poor viability, improved by inhibiting SR Ca(2+) release and mitochondrial Ca(2+) loading. PMID: 19959778
    34. role in cell communication PMID: 11889801
    35. Delta CaMK-II plays an important role in axonal development in embryonic cells. PMID: 12526889
    36. CaMKII is a downstream signal in CAN cardiomyopathy, and increased CaMKII activity contributes to cardiac dysfunction, arrhythmia susceptibility, and longevity during Ca2+-dependent phosphatase calcineurin overexpression. PMID: 16982937
    37. Interaction of calcium/calmodulin-dependent protein kinase IIdeltaC with sorcin indirectly modulates ryanodine receptor function in cardiac myocytes. PMID: 17707398
    38. In mouse and rabbit myocytes overexpressing CaMKIIdelta(C) by means of adenovirus-mediated gene transfer, recovery of fractional shortening and Ca transients was not impaired during late acidosis but even slightly improved. PMID: 17950750
    39. the data demonstrate a dynamic mechanism for CaMKII activation by oxidation and highlight the critical importance of oxidation-dependent CaMKII activation to AngII and ischemic myocardial apoptosis. PMID: 18455987
    40. The cloned, expressed CAMK2delta protein comigrated with D52 kinase and colocalized with D52 protein in T84 and HEK293 cells. These findings support a role for CAMK2delta in the mediation of D52 phosphorylation PMID: 18832449
    41. insulin regulates Myo1c function via CaMKII-dependent phosphorylation, and these events play a role in insulin-regulated GLUT4 PMID: 19046570
    42. the delta isoform of CaM kinase II is required for pathological cardiac hypertrophy and remodeling after pressure overload PMID: 19179290
    43. CaMKIIdelta contributes to cardiac decompensation by enhancing RyR2-mediated sarcoplasmic reticulum Ca2+ leak and that attenuating CaMKIIdelta activation can limit the progression to heart failure. PMID: 19381018
    44. CaMKII has both acute regulatory effects and chronic expression level effects on I(to) and I(K1) with complex consequences on cardiac myocyte action potential PMID: 19808479

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  • 亚细胞定位：
    Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 组织特异性：
    Expressed in cardiac muscle and skeletal muscle. Isoform Delta 2, isoform Delta 6, isoform Delta 6 and isoform Delta 10 are expressed in cardiac muscle. Isoform Delta 2 is expressed in skeletal muscle.
  • 数据库链接：

    KEGG: mmu:108058

    UniGene: Mm.255822