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  4. Recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit beta (Camk2b)

Recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit beta (Camk2b)

  • 货号：
    CSB-YP004466MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004466MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004466MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004466MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004466MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Camk2b; Camk2d; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; EC 2.7.11.17
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-542
  • 氨基酸序列
    MATTVTCTRF TDEYQLYEDI GKGAFSVVRR CVKLCTGHEY AAKIINTKKL SARDHQKLER EARICRLLKH SNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI LEAVLHCHQM GVVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKEA YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLIN QMLTINPAKR ITAHEALKHP WVCQRSTVAS MMHRQETVEC LKKFNARRKL KGAILTTMLA TRNFSVGRQT TAPATMSTAA SGTTMGLVEQ AKSLLNKKAD GVKPQTNSTK NSSAITSPKG SLPPAALEPQ TTVIHNPVDG IKESSDSTNT TIEDEDAKAR KQEIIKTTEQ LIEAVNNGDF EAYAKICDPG LTSFEPEALG NLVEGMDFHR FYFENLLAKN SKPIHTTILN PHVHVIGEDA ACIAYIRLTQ YIDGQGRPRT SQSEETRVWH RRDGKWQNVH FHCSGAPVAP LQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
  • 基因功能参考文献：
    1. Region-specific overexpression of betaCaMKII in dentate gyrus (DG) impairs behavioral flexibility, and severely attenuates the NMDAR-dependent long-term depression (LTD) in DG medial perforant path, which is further confirmed by the diminished AMPAR internalization and dephosphorylation during LTD. PMID: 27993520
    2. data suggest that phosphorylation of CaMKIIbeta through the mGluR/IP3R1/PKC signaling pathway represses excessive spine formation and elongation in mature Purkinje cells. PMID: 28607044
    3. The molecular, temporal and region-specific requirements of the CAMK2B in mouse locomotion have been described. PMID: 27244486
    4. Study used an unbiased semiquantitative proteomics approach to identify biologically relevant phosphorylation sites on both CaMKIIa and CaMKIIb PMID: 25650780
    5. CaMKK/CaMK IV pathway is a key endogenous protective mechanism in cerebral ischemia. PMID: 23868268
    6. Our data point toward a critical role of CaMKIIbeta in regulating oligodendrocyte maturation and CNS myelination via an actin-cytoskeleton-regulatory mechanism PMID: 23785157
    7. Muscle-specific CamkIIss isoform is critical for receptor recycling and may provide a mechanism by which the postsynaptic acetylcholine receptor density is maintained at the neuromuscular junction in vivo. PMID: 20844140
    8. is expressed in motor neurons during axon outgrowth and is part of slow axonal transport. PMID: 11891785
    9. accumulated in the nuclei of brain cells by pentylenetetrazol-mediated seizure via cellular mechanisms other than transcriptional and post-transcriptional regulation PMID: 11897160
    10. Ca/CaMK II activation plays an important role in the transmission of GnRH signals from the plasma membrane to the LH subunit genes. PMID: 12746302
    11. Expression of an active form of CaMKII beta after 1 day of T cell activation enhances initial proliferation and cytotoxic T cell activity, but induces profound unresponsiveness to peptide restimulation. PMID: 15843557
    12. activated CaMKII directly phosphorylated the recombinant COOH-terminal region of FAK at a residue equivalent to Ser-843 PMID: 15845548
    13. CaM kinase IIdelta2 was involved in the GnRH-induced activation of MAP kinase in GT1-7 cells. PMID: 17706588
    14. betaCaMKII activity in the dentate gyrus selectively impaired long-term potentiation in the dentate perforant path, but not in the CA1 Schaffer collateral pathway. PMID: 17913888
    15. We found that betaCaMKII, the predominant CaMKII isoform of the cerebellum, is important for controlling the direction of plasticity at the parallel fiber-Purkinje cell synapse PMID: 19503086
    16. Deletion studies indicated three important parts of beta CaM kinase II: beta-specific insertion 2, the association domain, and the oligomeric form; all 3 play an important role in its cellular distribution. PMID: 11532017

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 数据库链接：

    KEGG: mmu:12323

    UniGene: Mm.439733