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  4. Recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha (Camk2a)

Recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha (Camk2a)

  • 货号：
    CSB-YP004465MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004465MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004465MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004465MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004465MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Camk2aCalcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; EC 2.7.11.17
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-478
  • 氨基酸序列
    MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKNDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.; Has no kinase activity.
  • 基因功能参考文献：
    1. Fluorescence resonance energy transfer studies confirm that the extended state of CaMKII-alpha resolved by electron microscopy is the predominant form of the holoenzyme, even under molecular crowding conditions. PMID: 28589927
    2. Storage of passive motion pattern in hippocampal CA1 region depends on CaMKII/CREB signaling pathway in a motion sickness rodent model. PMID: 28230177
    3. Its pathway may promote reactive oxygen species generation in the early state Alzheimer's disease and eventually contribute to the exacerbation of pathological phenotype. PMID: 29602494
    4. Study showed the significance of TLR4 in upregulation of MAPK/ErK in NMDAR hypofunction schizophrenic-mouse model. This is characterized by a decrease in hippocampal IGF-1R/CaMKIIalpha, and social interaction behavioral deficits. Ultimately, hippocampal TLR4 knockdown rescued IGF-1R/CaMKIIalpha decline and prevented behavioral deficits by reducing MAPK/ErK expression. PMID: 29137928
    5. Hyperlipidemia induced by a high-fat diet was associated with increased expression and activity of CaMKII in heart. PMID: 28662005
    6. Data show that calcium/calmodulin protein kinase II (CaMKII)-mediated modulation of neuronal sodium current impacts neuronal excitability in SCN2A sodium channel alpha subunit Scn2a(Q54) mice and may represent a therapeutic target for the treatment of epilepsy. PMID: 28137877
    7. Results show that aging impairs the training-induced upregulation of autophosphorylated (T286) alphaCaMKII in the CA3 stratum radiatum of the hippocampus. This indicates that distinct age-related mechanisms underlie CFC that may rely more heavily on NMDA receptor-dependent plasticity in young age. PMID: 28648815
    8. This study found that the Camk2a gene showed a downregulated profile only in the Inflexible when compared to the Light ethanol drinkers group. PMID: 28714806
    9. Results suggest that the increased expression of calcium/calmodulin dependent protein kinase II in the lateral habenula is instrumental for morphine-driven aversive behaviors. PMID: 28527717
    10. our findings demonstrated that TINCR could attenuate myocardial hypertrophy by epigenetically silencing of CaMKII, which may provide a novel therapeutic strategy for cardiac hypertrophy. PMID: 28548932
    11. CaMKII is a target of PTENalpha phosphatase and that PTENalpha is an essential element in the molecular regulation of neural activity. PMID: 28636948
    12. In FHC-linked TnT-mutated hearts, RyR2 is susceptible to CaMKII-mediated phosphorylation, presumably because of a mutation-linked increase in diastolic [Ca(2+)]i, causing aberrant Ca(2+) release leading to lethal arrhythmia. PMID: 29402414
    13. Data suggest that alphaCaMKII autophosphorylation-dependent remodeling of glutamatergic synapses is a plausible mechanism for the regulation of the alcohol addiction-related behaviors. PMID: 26572936
    14. astrocytic IL-17A plays important roles in the maintenance of neuropathic pain through CaMKII/CREB signaling pathway in spinal cord. PMID: 26166359
    15. Wip1 phosphatase plays a vital role in regulating hippocampal synaptic plasticity by modulating the phosphorylation of CaMKII. PMID: 27158969
    16. Destabilization of PGC1a is attributable to decreased p38 MAPK activation via diminished CaMKII signaling. Thus, we elucidate a pathway downstream of Ca(2+)-mediated CaMKII activation that is dysfunctional in C3KO(Capn3 knock-out mice ) mice, leading to reduced transcription of genes involved in muscle adaptation PMID: 27005420
    17. In young mice, 30% of adult CaMKIIalpha expression is sufficient for normal long-term potentiation in the hippocampus and cerebral cortex. PMID: 27535377
    18. Findings demonstrate that Thr286 phosphorylation of CaMKII plays an important role in induction of long-term potentiation (LTP) by integrating Ca(2+) signals, and it greatly promotes, but is dispensable for, the activation of CaMKII and LTP. PMID: 28521133
    19. The present study demonstrates, for the first time, that ROS-dependent activation of CaMKII mediates altered Ca2+ handling and contractile dysfunction observed in the setting of sepsis. PMID: 27648519
    20. these data implicate the importance of subunit stoichiometry as another regulatory mechanism of PKA activity and lipid metabolism. PMID: 27496951
    21. Thus, taken into consideration the mechanism that controls the upregulation of maturation genes involved in synaptic formation, these results indicate that Etv1 orchestrates the activity-dependent regulation of both maturation and immaturation genes in developing granule cells and plays a key role in specifying the identity of mature granule cells in the cerebellum. PMID: 27059140
    22. Pharmacological and genetic studies using CaMKII antagonists and genetically modified [alpha]-CaMKII mice have shown that blockade or reduction of CaMKII reduces nicotine reward PMID: 26292186
    23. The study shows advanced glycation end products (AGEs) resulted from ribosylation activate calcium-/calmodulin-dependent protein kinase type II (CaMKII), a key kinase responsible for Tau hyperphosphorylation. PMID: 26095350
    24. HDAC2 may regulate the expression of immediate early genes, in part, by prolonging the actions of pCREB in the mouse nucleus accumbens. PMID: 26300473
    25. The results suggest that CaMKII-dependent TnI phosphorylation is involved in FDMCD and the consequent FDAR and that CaMKII inhibition removes this mechanism and thus induces diastolic dysfunction. PMID: 26297240
    26. Results indicate that alpha-Ca(2+)/calmodulin-dependent protein kinase II overexpression in the forebrain impairs behavioral flexibility and NMDAR-dependent long-term depression in the medial prefrontal cortex. PMID: 26415772
    27. As up-regulation of epithelial barriers is essential for the prevention of chronic inflammatory diseases, the identification of CaMKII as a modulator of TJ function paves the way for the development of new drugs to treat these diseases. PMID: 26281891
    28. Binge-like drinking decreased Camk2a phosphorylation in amygdala from adolescent mice, with no effect in adult mice. PMID: 26247621
    29. This study show that CaMKII regulates the flow of sensory information in Abeta-LTMR sensory neurons at multiple levels, including impulse generation, Action potential propagation, and dorsal horn synaptic transmission PMID: 26490852
    30. CaMKIIalpha selectively regulates mGluR1a and mGluR5a ERK1/2 signaling. PMID: 25885040
    31. Results identify CaMKII isoform phosphorylation sites and a network of synaptic protein interactions that are sensitive to the abrogation of Thr286 autophosphorylation of CaMKIIa PMID: 25650780
    32. Results suggest that inhibitory autophosphorylation of CAMKIIa regulates the threshold for induction of both long-term potentiation and long-term depression PMID: 24480420
    33. showed polypyrimidine tract binding protein (PTBP)-dependent alternative splicing of CaMKIIalpha transcripts in the lens PMID: 25540577
    34. Ca2+/calmodulin-dependent protein kinase-II (CaMKII) has a key role in the plasticity of glutamatergic synapses of the brain. PMID: 25290264
    35. VLDLR requires RasGRF1/CaMKII to alter dendritic spine formation. PMID: 25644714
    36. Genetic deletion of Camk2a impaired the locomotor response of mice to ecstasy. PMID: 26019340
    37. IRBIT suppresses CaMKIIalpha activity and contributes to catecholamine homeostasis through TH phosphorylation. PMID: 25922519
    38. Our results demonstrate unanticipated, fundamentally important roles for UNC-43/CaMKII in the regulation of synaptic strength. PMID: 25843407
    39. results provide new mechanistic insight into the role of alphakap in regulating the stability of the postsynaptic apparatus of neuromuscular synapses. PMID: 25834039
    40. data indicate that the surface expression and function of EAAT2b can be rapidly modulated through the disruption of its interaction with DLG1 by CaMKII activation. PMID: 25834051
    41. Angiotensin II activates PKA and CaMKII via NOX2, which results in disturbed sodium and calcium currents (via PKA) and enhanced diastolic sarcoplasmic reticulum Calcium leakage. PMID: 25073061
    42. Data show that resolvin D1 decrease nuclear 5-lipoxygenase (5-LOX) and the leukotrienes LTB4:LXA4 ratio by macrophages lacking calcium-sensitive kinase calcium-calmodulin-dependent protein kinase II (CaMKII). PMID: 25246560
    43. Therefore, these studies reveal a novel interaction between CAPN3 and CaM and identify CaM as the first positive regulator of CAPN3 activity PMID: 25389288
    44. Calmodulin and PI(3,4,5)P cooperatively bind to the Itk pleckstrin homology domain to promote efficient calcium signaling and IL-17A production. PMID: 25097034
    45. CaMKII-dependent phosphorylation of cardiac ryanodine receptors regulates cell death in cardiac ischemia/reperfusion injury. PMID: 24949568
    46. Aging increases both the oxidation and CaMKII-phosphorylation of RyR2, which results in calcium leak and atrial fibrillation initiation. PMID: 24576293
    47. This study demonistrated that the requirement for alphaCaMKII in the forebrain, but not the cerebellum, in spatial and contextual learning. PMID: 25143599
    48. Data indicate that nitric oxide directly affects Ca-calmodulin-dependent protein kinase (CaMKII) to sustain its activity leading to the increase in sarcoplasmic reticulum calcium leak. PMID: 24498331
    49. Loss of alpha-CaMKII from adult-generated granule cells is associated with impaired hippocampus-dependent learning. PMID: 25186740
    50. Our study presents novel evidence that CaMKII expression in vivo is regulated by a negative feedback loop following oxidative activation. PMID: 24418021

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  • 亚细胞定位：
    [Isoform Alpha KAP]: Cytoplasm.; Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendritic spine. Cell projection, dendrite.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 组织特异性：
    [Isoform Alpha CaMKII]: Expressed in brain.; [Isoform Alpha KAP]: Expressed in skeletal muscle.
  • 数据库链接：

    KEGG: mmu:12322

    STRING: 10090.ENSMUSP00000099952

    UniGene: Mm.131530