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  4. Recombinant Mouse Bromodomain-containing protein 2 (Brd2)

Recombinant Mouse Bromodomain-containing protein 2 (Brd2)

  • 货号：
    CSB-YP741953MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP741953MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP741953MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP741953MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP741953MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Brd2; Fsrg1; Kiaa4005; Ring3; Bromodomain-containing protein 2; Female sterile homeotic-related protein 1; Fsrg-1; Protein RING3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-798
  • 氨基酸序列
    MLQNVTPHKL PGEGNAGLLG LGPEAAAPGK RIRKPSLLYE GFESPTMASV PALQLAPANP PPPEVSNPKK PGRVTNQLQY LHKVVMKALW KHQFAWPFRQ PVDAVKLGLP DYHKIIKQPM DMGTIKRRLE NNYYWAASEC MQDFNTMFTN CYIYNKPTDD IVLMAQTLEK IFLQKVASMP QEEQELVVTI PKNSHKKGAK LAALQGSITS AHQVPAVSSV SHTALYTPPP EIPTTVLNIP HPSVISSPLL KSLHSAGPPL LAVSAAPPAQ PLAKKKGVKR KADTTTPTPT AILAPGSPAS PPGSLEPKAA RLPPMRRESG RPIKPPRKDL PDSQQQHQSS KKGKLSEQLK HCNGILKELL SKKHAAYAWP FYKPVDASAL GLHDYHDIIK HPMDLSTVKR KMENRDYRDA QEFAADVRLM FSNCYKYNPP DHDVVAMARK LQDVFEFRYA KMPDEPLEPG PLPVSTALPP GLTKSSSESS SEESSSESSS EEEEEEEEDE DEEESESSDS EEERAHRLAE LQEQLRAVHE QLAALSQGPI SKPKRKREKK EKKKKRKAEK HRGRIGIDED DKGPRAPRPP QPKKSKKAGG GGSNATTLSH PGFGTSGGSS NKLPKKSQKT APPVLPTGYD SEEEEESRPM SYDEKRQLSL DINKLPGEKL GRVVHIIQAR EPSLRDSNPE EIEIDFETLK PSTLRELERY VLSCLRKKPR KPYTIRKPVG KTKEELALEK KRELEKRLQD VSGQLNSTKK PPKKASEKTE SSAQQVAVSR LSASSSSSDS SSSSSSSSSS DTSDSDSG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly. May play a role in spermatogenesis or folliculogenesis.
  • 基因功能参考文献：
    1. these results provided evidence for a novel role of Brd2 in chronic inflammation and insulin resistance. PMID: 27865874
    2. Brd2 and Brd4 genetic transcription required for Th17 cell development and adaptive immunity. PMID: 28262505
    3. This study reveals an antagonistic relationship between H2A.Z.1ub and BRD2 to regulate the transcriptional balance at bivalent genes to enable proper execution of developmental programs. PMID: 26804911
    4. BRD2 is involved in the modulation of neuroinflammatory responses through PAI-1 and via the regulation of epigenetic reader BET protein PMID: 26349765
    5. DNA demethylation of the Brd2 promoter region induced Brd2 expression during differentiation of 3T3-L1 cells into adipocytes. PMID: 25575605
    6. Brd2 inhibits adipogenesis via the ERK1/2 signaling pathway in 3T3-L1 adipocytes. PMID: 24194944
    7. A structural basis for BRD2/4-mediated host chromatin interaction and oligomer assembly of Kaposi sarcoma-associated herpesvirus and murine gammaherpesvirus LANA proteins. PMID: 24146614
    8. We conclude that Brd2 plays a critical, independent role in regulation of mitogenic response genes PMID: 24319289
    9. Data show that infection of macrophages with Listeria monocytogenes caused binding of the BET proteins Brd2, Brd3, and, most prominently, Brd4 to the Nos2 promoter. PMID: 24248598
    10. Identification of motif B of Brd2 as a dimerization domain involved in association to mitotic chromosomes and proper recognition of acetylated chromatin. PMID: 22595521
    11. Studies showed that although Brd2 mRNA is expressed in both the hippocampus and cerebellum, Brd2 protein only can be detected in the cerebellar Purkinje cells and not in hippocampal cells. PMID: 21608014
    12. Compared to the Brd2+/+ littermates, Brd2+/- males have a decreased clonic, and females a decreased tonic-clonic, seizure threshold. PMID: 21887291
    13. Data show that hypomorphic Brd2 shifts energy balance toward storage without causing glucose intolerance and may provide a novel model for obese metabolically healthy humans. PMID: 19883376
    14. may be involved in the transcriptional activities of genes involved in proliferation of the mammary epithelia during pregnancy PMID: 12145330
    15. The distinct patterns of Brd2 localization within the adult ovary supports a role for Brd2 in mitotic and possibly meiotic cell cycle regulation PMID: 15112318
    16. Brd2-deficient embryos could not be rescued by wild-type extraembryonic tissues PMID: 19301389
    17. In developing a mouse model deficient in Brd2, we find that Brd2 is required for the completion of embryogenesis and proper neural tube closure during development. PMID: 19362612
    18. Knockdown of Brd2 with shRNA promotes adipogenic differentiation of 3T3-L1 preadipocytes in vitro. PMID: 19883376
    19. Reduced Brd2 levels block macrophage infiltration of white adipose tissue in mice and inflammation-associated insulin resistance. PMID: 19883376
    20. Brd2 inhibits pancreatic beta cell growth and insulin transcription. PMID: 19883376
    21. Knockdown of Brd2 with shRNA protects 3T3-L1 adipocytes from TNF-alpha-induced insulin resistance. PMID: 19883376
    22. Brd2 co-represses PPAR-gamma in 3T3-L1 pre-adipocytes and inhibits adipogenesis. PMID: 19883376
    23. Brd2 deficiency causes extreme obesity but simultaneously protects against whole-body insulin resistance and Type 2 diabetes. PMID: 19883376
    24. The Brd2 gene is located on Chromosome 17, within a region syntenic to the human class II major histocompatibility locus, but appears to have no role in antigen processing; Brd2 is likely involved in signal transduction and transcription. PMID: 11983150
    25. Brd2 autophosphorylation activity is induced in mouse tissues (lung, kidney, brain, liver and heart) upon systemic treatment with the mitogens Epidermal Growth Factor or Phorbol 12-Myristate 13-Acetate. PMID: 9528865

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Predominantly expressed in the testis, followed by ovary, placenta, embryo and to a lower extent in somatic tissues.
  • 数据库链接：

    KEGG: mmu:14312

    STRING: 10090.ENSMUSP00000025193

    UniGene: Mm.3444