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  4. Recombinant Mouse Beclin-1 (Becn1)

Recombinant Mouse Beclin-1 (Becn1)

In Stock
  • 货号：
    CSB-EP002658MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Becn1; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    67.6kDa
  • 表达区域：
    1-448aa
  • 氨基酸序列
    MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEEANSGEEPFIETRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQVRYAQIQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in autophagy. Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis including endosome formation in neuronal cells. May play a role in antiviral host defense.; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
  • 基因功能参考文献：
    1. data demonstrate that disruption of the beclin 1-BCL2 complex is an effective mechanism to increase autophagy, prevent premature ageing, improve healthspan and promote longevity in mammals PMID: 29849149
    2. HER2 interacts with Beclin 1 in breast cancer cells and inhibits autophagy. Mice with increased basal autophagy due to a genetically engineered mutation in Becn1 are protected from human HER2-driven mammary tumorigenesis, and HER2 fails to inhibit autophagy in primary cells derived from these mice. PMID: 29610308
    3. Catalase ameliorates diabetes-induced cardiomyopathy through reduced RelA-mediated transcription of BECN1. PMID: 28643395
    4. TRIM50, via the RING domain, ubiquitinates Beclin 1 in a K63-dependent manner enhancing its binding with ULK1 and autophagy activity. PMID: 29604308
    5. findings suggested E. faecalis LTA may increased macrophages autophagy via inhibiting PI3K/Akt/mTOR pathway and this process was Beclin1 dependent. PMID: 29551680
    6. The results suggest that SPK2 interacts with Bcl2 via its BH3 domain, thereby dissociating it from Beclin-1 and activating autophagy while protecting neurons against ischemic injury. PMID: 28682313
    7. alpha-asarone abrogated 7beta-hydroxycholesterol-triggered eIF2alpha-CHOP activation, and enhanced macrophage beclin1 dependent autophagy. PMID: 28088783
    8. These results suggest that the phosphorylated CAV1 functions to activate autophagy through binding to the BECN1/VPS34 complex under oxidative stress and to protect against ischemic damage. PMID: 28542134
    9. Intracerebral hemorrhage contributes to microglia autophagic activation through BECN1 (and ATG5) pathway. PMID: 26732594
    10. These data demonstrate the essential role of Beclin-1 in the formation of autophagosomes and lysosome biogenesis and underline that deletion of this key system is deleterious for cell viability. PMID: 26456737
    11. AMP-activated protein kinase (AMPK) regulates autophagy by phosphorylating BECN1 at Thr388 PMID: 27304906
    12. BECN1/Beclin 1 binding to viral phosphoprotein (P) induced an incomplete autophagy via activating the pathways CASP2-AMPK-AKT-MTOR and CASP2-AMPK-MAPK by decreasing CASP2. PMID: 28129024
    13. proteostasis regulator cysteamine, which rescues the function of the most common F508del-CFTR mutant and hence reduces lung inflammation in CF patients, can also repair the defects of CF macrophages, thus restoring both bacterial internalization and clearance through a process that involves upregulation of the pro-autophagic protein Beclin 1 and re-establishment of the autophagic pathway. PMID: 28079883
    14. Genetically disrupting BECN1-BCL2 binding hyperactivates autophagy in vivo, which sequestrates amyloid oligomers and prevents Alzheimer's disease progression. PMID: 28806762
    15. Data show that 1-month of oral treatment with beta-asarone reduces AChE, Abeta42, APP and Beclin-1 levels and alleviates some behavioral impairments by inhibiting the autophagy via regulating the PI3K/Akt/mTOR pathway in APP/PS1 transgenic mice. The results further support the exploration of beta-asarone as a possible disease-modifying agent for the treatment of Alzheimer's disease. PMID: 27737765
    16. identify FAK as a novel negative regulator of Beclin1-mediated autophagy and indicate that this pathway can facilitate the promotion of compensatory hypertrophic growth PMID: 27994061
    17. The results not only demonstrate that BECN1 as a PI3K-III component is essential for cytokinesis, but also suggest that BECN1 is not associated with autophagy pathway in mouse oocytes. PMID: 27149384
    18. this study shows that knockdown of beclin1 enhances macrophage-associated phagocytosis and intracellular killing of Pseudomonas aeruginosa PMID: 27295610
    19. provide the novel evidence that GADD45A inhibits autophagy via impairing the BECN1-PIK3C3 complex formation PMID: 26636486
    20. in-vitro genetic depletion of beclin-1 in high glucose treated adult rat cardiomyocytes markedly inhibited the level of autophagy and subsequent apoptotic cell death. PMID: 26386349
    21. suggest that neuronal death induced by decreased beclin 1 levels may also be due to impaired trophic factor signaling PMID: 26692002
    22. Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex. PMID: 25275521
    23. a mechanism of inverse coregulation between BECN1 and MCL1 significantly contributes to their opposing roles in tumorigenesis PMID: 25837021
    24. FKBP5 associates with BECN1, changes its phosphorylation and protein levels and enhances markers of autophagy and autophagic flux PMID: 25714272
    25. identifies a novel negative regulator of autophagy and suggests that RNF216 may be a target for treatment of inflammatory diseases PMID: 25484083
    26. Inverse co-regulation of Beclin 1 and Mcl-1 represents a mechanism of functional counteraction in cancer. PMID: 25472497
    27. Beclin 1-mediated autophagy alleviates cerebral ischemic injury following down-regulation of miRNA-30a. PMID: 24771295
    28. CFTR and BECN1 function can be restored with cysteamine and EGCG in a model of the F508del-CFTR mutation PMID: 25350163
    29. a neuroprotective role for ARRB1, in the context of cerebral ischemia, centered on the regulation of BECN1-dependent autophagosome formation PMID: 24988431
    30. Data show that beclin1 (Becn1) provides essential functions in the ovary that are essential for mammalian reproduction. PMID: 25246579
    31. ALDH2 deficiency exacerbates the pressure overload induced cardiac dysfunction partly by inhibiting Beclin-1 dependent autophagy pathway. PMID: 25086229
    32. These data support a dual role of BECN1 in amyotrophic lateral sclerosis. PMID: 24905722
    33. Becn1 heterozygous knockout mice displayed a prolonged bleeding time and reduced platelet aggregation PMID: 24458007
    34. Our findings demonstrate a novel protective effect of CO in sepsis, dependent on autophagy protein Beclin 1, in a murine model of CLP-induced polymicrobial sepsis PMID: 23971531
    35. Beclin1 is involved, and might be responsible for the crosstalk between autophagy and apoptosis in mouse odontogenesis. PMID: 23793984
    36. Suppression of the Beclin-1-dependent pathway reduced autophagy in pressure-overload heart failure. PMID: 24550138
    37. Rhes robustly binds the autophagy regulator Beclin-1, decreasing its inhibitory interaction with Bcl-2 independent of JNK-1 signaling. PMID: 24324270
    38. Mst1 promoted cardiac dysfunction in mice subjected to myocardial infarction by inhibiting autophagy, associated with increased levels of Thr108-phosphorylated Beclin1. PMID: 24141421
    39. beclin 1 is required for efficient phagocytosis in a microglial cell line and in mouse brains. PMID: 24012002
    40. ATG (BECN1 or ATG7) knockdown compromised mammary epithelial cell-mediated apoptotic body clearance in association with decreased RAC1 activation. PMID: 23380905
    41. Lack of the beclin-binding domain increases autophagy response and activation of NLRP3 inflammasome, which in turn induces a more rapid innate immune response in HSV-1 infection. PMID: 23611643
    42. Caspase 1 activation is protective against hepatocyte cell death by up-regulating beclin 1 protein and mitochondrial autophagy in the setting of redox stress. PMID: 23589298
    43. Attenuation of autophagy by a knockdown of beclin-1 enhanced neurons susceptibility to proapoptotic signals induced by amino acid starvation. PMID: 23011054
    44. IL-2 induced HMGB1 cytoplasmic translocation in MEFs and promoted interaction between HMGB1 and beclin1, which is required for autophagy induction. PMID: 23195496
    45. Aerosol delivery of beclin1 enhanced the anti-tumor effect of radiation in the lungs of K-rasLA1 mice PMID: 22843615
    46. Bim inhibits autophagy by interacting with Beclin 1, an autophagy regulator, an interaction facilitated by LC8. PMID: 22742832
    47. Ischemia/reperfusion injury impairs autophagosome clearance mediated in part by reactive oxygen species-induced decline in lysosome-associated membrane protein-2 and upregulation of BECLIN-1, contributing to increased cardiomyocyte death. PMID: 22592897
    48. in Escherichia coli-containing phagosomes of mouse macrophages, Slamf1 interacts with the class III PI3K Vps34 in a complex with Beclin-1 and UVRAG PMID: 22493499
    49. Beclin 1 coordinates actin dynamics and membrane phospholipid synthesis to promote efficient apoptotic cell engulfment with Rac1 PMID: 22393062
    50. Beclin 1 is involved in regulation of apoptosis and autophagy during replication of ectromelia virus in permissive L929 cells. PMID: 21972018

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  • 亚细胞定位：
    Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome. Cytoplasmic vesicle, autophagosome.; [Beclin-1-C 35 kDa]: Mitochondrion. Nucleus. Cytoplasm.; [Beclin-1-C 37 kDa]: Mitochondrion.
  • 蛋白家族：
    Beclin family
  • 数据库链接：

    KEGG: mmu:56208

    STRING: 10090.ENSMUSP00000119369

    UniGene: Mm.178947