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  4. Recombinant Mouse BRCA1-associated protein (Brap)

Recombinant Mouse BRCA1-associated protein (Brap)

  • 货号：
    CSB-YP860396MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP860396MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP860396MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP860396MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP860396MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BrapBRCA1-associated protein; EC 2.3.2.27; BRAP2; Impedes mitogenic signal propagation; IMP; RING-type E3 ubiquitin transferase BRAP2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-591
  • 氨基酸序列
    MSVSLVVIRL ELAGHSPVPT DFGFSAAAGE MSDEEIKKKT LASAVACLEG KSAGEKAAII HQHLGRREMT DVIIETMKAR ADEVRDTVEE KKPSAAPVSA QRSREQSESV NTAPESPSKQ LPDQISFFSG NPSVEIVHGI MHLYKTNKMT SLKEDVRRSA MLCVLTVPAT MTSHDLMKFV APFNDVIEQM KIIRDSTPNQ YMVLIKFSAQ ADADSFYMAC NGRQFNSIED DVCQLVYVER AEVLKSEDGA SLPVMDLTEL PKCTVCLERM DESVNGILTT LCNHSFHSQC LQRWDDTTCP VCRYCQTPEP VEENKCFECG VQENLWICLI CGHIGCGRYV SRHAYKHFEE TQHTYAMQLT NHRVWDYAGD NYVHRLVASK TDGKIVQYEC EGDTCQEEKI DALQLEYSYL LTSQLESQRI YWENKIVRIE KDTAEEINNM KTKFKETIEK CDSLELRLSD LLKEKQSVER KCTQLNTRVA KLSTELQEEQ ELNKCLRANQ LVLQNQLKEE EKLLKETCAQ KDLQITEIQE QLRDVMFYLE TQQQISHLPA ETRQEIQEGQ INIAMASAPN PPSSGAGGKL QSRKGRSKRG K
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
  • 基因功能参考文献：
    1. These findings demonstrate that neuronal differentiation is potentiated during G1 phase by Brap-directed cascade of events in cell signaling and protein turnover. PMID: 28768199
    2. Together the results indicate for the first time that BRAP2 may play an important NRNI role in germ cells of the testis, with an additional, scaffold/structural role in mature spermatozoa. PMID: 25820252
    3. ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952
    4. A molecular complex composed of Nde1, Lis1, and Brap regulates the dynamic MAPK signaling threshold in a spatially dependent fashion. PMID: 23673330
    5. p21 Ras/Imp regulate cytokine production and migration in CD4 T cells PMID: 18577512

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  • 亚细胞定位：
    Cytoplasm.
  • 组织特异性：
    Isoform 2 is highly expressed in testis, lower levels in brain, heart, lung, stomach, colon, uterus, liver and kidney. Isoform 1 is only expressed in the testis. Isoform 2 is predominant over isoform 1 in both fetal and adult testis.
  • 数据库链接：

    KEGG: mmu:72399

    STRING: 10090.ENSMUSP00000031414

    UniGene: Mm.153372