Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse B-cell lymphoma 6 protein homolog (Bcl6)

Recombinant Mouse B-cell lymphoma 6 protein homolog (Bcl6)

  • 货号：
    CSB-YP002625MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002625MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002625MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002625MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002625MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Bcl6; Bcl-6; B-cell lymphoma 6 protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-707
  • 氨基酸序列
    MASPADSCIQ FTRHASDVLL NLNRLRSRDI LTDVVIVVSR EQFRAHKTVL MACSGLFYSI FTDQLKCNLS VINLDPEISP EGFCILLDFM YTSRLNLREG NIMAVMTTAM YLQMEHVVDT CRKFIKASEA EMAPALKPPR EEFLNSRMLM PHDIMAYRGR EVVENNMPLR NTPGCESRAF APPLYSGLST PPASYPMYSH LPLSTFLFSD EELRDAPRMP VANPFPKERA LPCDSARQVP NEYSRPAMEV SPSLCHSNIY SPKEAVPEEA RSDIHYSVPE GPKPAVPSAR NAPYFPCDKA SKEEERPSSE DEIALHFEPP NAPLNRKGLV SPQSPQKSDC QPNSPTESCS SKNACILQAS GSPPAKSPTD PKACNWKKYK FIVLNSLNQN AKPEGSEQAE LGRLSPRAYP APPACQPPME PANLDLQSPT KLSASGEDST IPQASRLNNL VNRSLAGSPR SSSESHSPLY MHPPKCTSCG SQSPQHTEMC LHTAGPTFPE EMGETQSEYS DSSCENGTFF CNECDCRFSE EASLKRHTLQ THSDKPYKCD RCQASFRYKG NLASHKTVHT GEKPYRCNIC GAQFNRPANL KTHTRIHSGE KPYKCETCGA RFVQVAHLRA HVLIHTGEKP YPCEICGTRF RHLQTLKSHL RIHTGEKPYH CEKCNLHFRH KSQLRLHLRQ KHGAITNTKV QYRVSAADLP PELPKAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
  • 基因功能参考文献：
    1. Bcl-6, expressed in abundance in Tfr cells, inhibits CD25 expression and IL-21-mediated inhibition of CD25 is Bcl-6 dependent. PMID: 28303891
    2. Together these results identify BCL6 as a potential driver of ETV6-RUNX1-mediated leukemogenesis, which could involve loss of BTG1-dependent suppression of ETV6-RUNX1 function. PMID: 29408281
    3. this study shows that IL4 and IL21 cooperate to induce the high Bcl6 protein level required for germinal center formation PMID: 28875978
    4. Although BCL6 controls follicular helper T cells activity in humans and mice, the role of miR-31 is restricted to human follicular helper T cell differentiation, reflecting a species specificity of the miR-31 action. PMID: 29133396
    5. These findings indicate a role of the interaction between TH2-promoting factors and Bcl6 in promoting appropriate IL-4 production in mTH2 cells and suggest that chronic allergic diseases involve the TH2-promoting factor-mediated functional breakdown of Bcl6, resulting in allergy exacerbation. PMID: 28096407
    6. Bioinformatics analysis and the dual-luciferase reporter assay demonstrated that miR-10b could target the 3'-untranslated regions of B cell lymphoma 6 (Bcl6) which is an important regulator of osteoblast differentiation. PMID: 28440396
    7. Def6 limits proliferation of T follicular helper cells in mice via alteration of mTORC1 signaling and inhibition of Bcl6 expression. PMID: 28811467
    8. The balance between CD4(+) cytotoxic T cell and follicular helper T(Tfh) differentiation heavily depends on the class of infecting virus and is jointly regulated by the Tfh-related transcription factors Bcl6 and Tcf7 (encoding TCF-1) and by the expression of the inhibitory receptors PD-1 and LAG3. PMID: 27806296
    9. Study provides evidence for an essential role of Bcl6 in the complex regulation of gene transcription during early adipogenesis. The action of Bcl6 is at least partially mediated by the direct transcriptional activation of STAT1. PMID: 27251748
    10. These data describe a novel regulatory mechanism through which STAT3 and the Ikaros zinc finger transcription factors Aiolos and Ikaros cooperate to regulate Bcl-6 expression. PMID: 28848064
    11. Our data reveal a regulatory role of BCL6 in inhibiting antiviral resistance factors in follicular Th cells PMID: 28550121
    12. data provide a novel mechanism for positive control of gene expression by Bcl6, and illuminate how Bcl6 and Blimp1 control follicular helper T cell differentiation PMID: 28586108
    13. both mouse and human B cells, IFN-gamma synergized with B cell receptor, toll-like receptor, and/or CD40 activation signals to promote cell-intrinsic expression of the GC master transcription factor, B cell lymphoma 6 protein. PMID: 27069113
    14. observations reveal that KLF6 repress BCL6 to enhance macrophage inflammatory gene expression and function. PMID: 27539853
    15. synergistic activity of Card11 mutant and Bcl6 in the development of diffuse large B-cell lymphoma in a mouse model PMID: 27560392
    16. Bcl6, by interacting with the co-factors NcoR2 and HDAC3, plays a pivotal role in controlling IRF7 induction and antiviral signaling priming. PMID: 26728228
    17. Bcl6 promoted T follicular helper cell differentiation through antagonizing IL-7R / STAT5a axis. PMID: 26876184
    18. Bob1 directly binds to and transactivate the Bcl6 promoter during the development of follicular T helper cells . PMID: 26957522
    19. upon binding to a composite NFAT/BCL6 regulatory element within the Ccl2 promoter, NFATc1/beta proteins release the BCL6-dependent repression of Ccl2 gene in macrophages. PMID: 26631626
    20. transferred Bcl6-deficient T cells into wildtype hosts. Bcl6-deficient T cells did not develop into GC Tfh, but they still generated CXCR5+ IFN-gamma+ IL-21+ IL-10+ Teff, suggesting that this predominant population is not of the Tfh-lineage PMID: 26646149
    21. Bcl6 middle domain repressor function is required for T follicular helper cell differentiation and utilizes the corepressor MTA3. PMID: 26460037
    22. Data indicate that signal transducer and activator of transcription 3 (Stat3) can repress the expression of transcription factor Bcl6 and interleukin-4 (IL-4) in follicular helper T (TFH) cells. PMID: 26188063
    23. Enforced expression of BCL6 in EGR2/3-deficient CD4 T cells partially restored Tfh differentiation and GC formation in response to virus infection PMID: 25979336
    24. PRMT7 could recruit H4R3me1 and symmetric H4R3me2 to the Bcl6 promoter. These results provide evidence for the important roles played by PRMT7 in germinal center formation. PMID: 26179907
    25. Blimp1 (encoded by Prdm1) inhibits T(FH) differentiation by antagonizing Bcl-6. TCF-1 promoted Bcl-6 expression but repressed Blimp1 expression. PMID: 26214740
    26. Transiently expressing Bcl6 in HSPCs causes mature B-cell lymphomas that lack Bcl6 expression and target-gene repression, are transcriptionally similar to post-GCB cells, and show epigenetic changes that are conserved from HSPCs to mature B-cells. PMID: 24887457
    27. these data suggest that the Bcl6 BTB domain is a key mediator of the differentiation of Tfh cells. PMID: 25957170
    28. ICOS and Bcl6-dependent pathways maintain a CD4 T cell population with memory-like properties during tuberculosis. PMID: 25918344
    29. lack of Bcl6 resulted in significant elevation of Stat1 mRNA and protein expression in osteoblasts, and Stat1 is a direct target of Bcl6 PMID: 25597995
    30. These data show both Treg-intrinsic and Treg-extrinsic roles for Bcl6 in controlling Treg cell stability and Th2 inflammation, and support the idea that Bcl6 expression in Treg cells is critical for controlling Th2 responses. PMID: 25262912
    31. miR-155 inhibits macrophage proliferation by suppressing Csf1r in early stages and decreased efferocytosis by targeting Bcl6 in advanced atherosclerosis. PMID: 25810298
    32. RD2 mutant B cells fail to form germinal centes and recruit Hdac2. PMID: 25176650
    33. Thus transcriptional outcomes on GH-regulated genes occupied by BCL6 and STAT5 are determined in a promoter-specific fashion by co-regulatory proteins which mediate the distinction between activating and repressive transcription factors. PMID: 25088465
    34. IRF8 pairs with BCL6 co-repressor BCOR in the nucleus to enhance its transcriptional repressive activity. PMID: 25331958
    35. Regulation of Bcl6 protein levels is dynamic in murine conventional dendritic cells during development, maturation and activation in vivo. PMID: 24979752
    36. Bcl6 plays a role in regulatory events of lipid metabolism. PMID: 24892698
    37. Data indicate that the transcription factor Bcl-6 directly repressed genes encoding molecules involved in the glycolysis pathway. PMID: 25194422
    38. survival of CXCR5(+) follicular helper T memory cells, and thus subsequent recall antibody response, require Bcl6 expression PMID: 25071203
    39. Data show that Bcl6 (B cell lymphoma 6)-cullin 3 complexes provide essential negative feedback regulation during both thymocyte development and T cell activation to restrain excessive T follicular helper (Tfh) responses. PMID: 24863065
    40. In experimental autoimmune encephalomyelitis, forced expression of BCL6 in myelin oligodendrocyte glycoprotein35-55-specific Th9 cells resulted in decreased IL-9 production and induction of IFN-gamma PMID: 24879792
    41. Increasing BCL6 expression inhibits IL-9 production and decreases efficient Th9 differentiation. PMID: 24550509
    42. derepressed Bcl6 expression may be responsible for increases in germinal centers cell proliferation and splenomegaly of Kaiso knockout mice. PMID: 24269670
    43. BLIMP1 deficiency and Bcl6 overexpression induce lymphoma in mice that is prevented by T cell-mediated tumor surveillance PMID: 24487434
    44. The induction of Bcl6 was regulated directly by STAT1, which bound to the Bcl6, Cxcr5, and Pdcd1 loci. PMID: 24489092
    45. our study highlights two novel mechanisms for how Bcl6 controls the TFH cell lineage: 1) by repressing IL-10 expression, and 2) by inhibiting apoptosis of TFH cells. PMID: 23980208
    46. B-cell lymphoma 6 is a negative regulator of interleukin-17-producing T-helper cell differentiation, and thus a potential target of miR-212. PMID: 23818645
    47. MiR-21 as a critical target of Bcl6. PMID: 23416424
    48. SWAP-70 controls IgE production through regulation of the antagonistic STAT6 and BCL6 occupancy of the germline IgE promoter PMID: 23589612
    49. CD4 and CD8 T cells share core aspects of a memory cell precursor gene expression program involving Bcl6. PMID: 23487426
    50. The repression of inflammatory responses by Bcl-6 in macrophages was accordingly independent of the repressor function of the BTB domain. Bcl-6 thus mediates its actions through lineage-specific biochemical functions. PMID: 23455674

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed at least in germinal center B-cells of spleen.
  • 数据库链接：

    KEGG: mmu:12053

    STRING: 10090.ENSMUSP00000023151

    UniGene: Mm.347398