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  4. Recombinant Mouse Arachidonate 5-lipoxygenase (Alox5)

Recombinant Mouse Arachidonate 5-lipoxygenase (Alox5)

  • 货号：
    CSB-YP001624MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001624MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001624MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001624MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001624MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Alox5; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase; EC 1.13.11.34
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-674
  • 氨基酸序列
    MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGA VDSYDVTVDE ELGEIYLVKI EKRKYWLHDD WYLKYITLKT PHGDYIEFPC YRWITGEGEI VLRDGRAKLA RDDQIHILKQ HRRKELEARQ KQYRWMEWNP GFPLSIDAKC HKDLPRDIQF DSEKGVDFVL NYSKAMENLF INRFMHMFQS SWHDFADFEK IFVKISNTIS ERVKNHWQED LMFGYQFLNG CNPVLIKRCT ALPPKLPVTT EMVECSLERQ LSLEQEVQEG NIFIVDYELL DGIDANKTDP CTHQFLAAPI CLLYKNLANK IVPIAIQLNQ TPGESNPIFL PTDSKYDWLL AKIWVRSSDF HVHQTITHLL RTHLVSEVFG IAMYRQLPAV HPLFKLLVAH VRFTIAINTK AREQLICEYG LFDKANATGG GGHVQMVQRA VQDLTYSSLC FPEAIKARGM DSTEDIPFYF YRDDGLLVWE AIQSFTMEVV SIYYENDQVV EEDQELQDFV KDVYVYGMRG KKASGFPKSI KSREKLSEYL TVVIFTASAQ HAAVNFGQYD WCSWIPNAPP TMRAPPPTAK GVVTIEQIVD TLPDRGRSCW HLGAVWALSQ FQENELFLGM YPEEHFIEKP VKEAMIRFRK NLEAIVSVIA ERNKNKKLPY YYLSPDRIPN SVAI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the oxygenation of arachidonate to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonic acid into 8-hydroperoxyicosatetraenoic acid (8-HPETE) and 12-hydroperoxyicosatetraenoic acid (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma)(PubMed:21307302). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells.
  • 基因功能参考文献：
    1. it was suggested that 5-LO in monocytes played a pivotal role in monocyte-macrophage differentiation and subsequent infiltration of macrophage in neointima, leading to vascular remodeling after vascular injury. PMID: 28645655
    2. the up-regulation of the ALOX5 is responsible for the Homocysteine-dependent worsening of the Alzheimer's disease phenotype in a relevant mouse model of the disease. PMID: 28383037
    3. Our data showed that besides the high parasite burden and lack of microbicidal molecules, an imbalance with high COX-2 and 5-LOX eicosanoid expression and a lack of regulatory PPAR-gamma cytoplasm-to-nucleus translocation in macrophages were observed in mice that develop cerebral malaria. PMID: 27887739
    4. Data indicate that 5-lipoxygenase (5-LO)/leukotriene B4 (LTB4) axis orchestrates graft-versus-host disease (GVHD) development and suggest it could be a target for the development of therapeutic strategies for GVHD treatment. PMID: 28947611
    5. Findings demonstrate that the up-regulation of the ALOX5 gene pathway is responsible for the development of the biochemical and behavioral sequelae of high Hcy brain levels in the context of a neurodegenerative phenotype. PMID: 28334897
    6. This study demonstrates that LTB4 promotes macrophage phagocytosis of bacteria via BLT1, and that BLT2 can fulfill this role in the absence of BL PMID: 28053185
    7. our results define Alox5 as a key genetic effector of JAK2V617F in driving polycythemia vera PMID: 27784744
    8. Homocysteine directly influences 5LO expression levels and establish a previously unknown cross talk between these two pathways. PMID: 27896923
    9. Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase. PMID: 27060751
    10. Data suggest that miR-674-5p (microRNA-674-5p) serves as a negative regulator in 5-LO (arachidonate 5-lipoxygenase) mediated autoimmune liver injury; miR-674-5p represses expression of 5-LO in hepatocytes in the presence of IL-6 (interleukin-6) or TNFa (tumor necrosis factor-alpha). PMID: 27313091
    11. Results establish a key role of 5-Lipoxygenase in the development of the tau pathology phenotype and demonstrate it to be a novel viable therapeutic target for the pharmacologic treatment of human tauopathy. PMID: 25802082
    12. GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
    13. SHH-responsive 5-lipoxygenase, 15-lipoxygenase and COX-2 modulate Dectin-1-induced inflammatory cytokines. PMID: 26432261
    14. involvement of lipoxygenase pathways in TNF-alpha-induced production of cytokines and chemokines PMID: 25229347
    15. explored the relationship between 5-LO and central and peripheral eosinophilia in an asthma model using PAS or BALB/c mice and 5-LO-deficient mutants PMID: 25477712
    16. ALOX5 deficiency prevents stress-induced memory deficits, synaptic dysfunction and tauopathy in a mouse model of Alzheimer's disease. PMID: 25122659
    17. 5-LO activation coordinates the inflammatory immune response involved in the control of visceral leishmaniasis. PMID: 25309905
    18. 5-LO(-/-) mice subjected to kidney insult presented lower macrophage infiltration and higher levels of IL-10 than wild-type mice. PMID: 25302946
    19. Alox5 affects the osteogenic and adipogenic abilities of iPSCs in vivo and the effect of Cox2 inhibition in this system. PMID: 24906289
    20. Data show that resolvin D1 decrease nuclear 5-lipoxygenase (5-LOX) and the leukotrienes LTB4:LXA4 ratio by macrophages lacking calcium-sensitive kinase calcium-calmodulin-dependent protein kinase II (CaMKII). PMID: 25246560
    21. abdominal aortic aneurysm formation was attenuated in 5-LO(-/-) mice, and in lethally irradiated wild-type mice reconstituted with 5-LO(-/-) bone marrow in an elastase perfusion model PMID: 25324573
    22. 5-Lipoxygenase negatively regulates Th1 response during Brucella abortus infection in mice. PMID: 25583526
    23. This study showed that a novel functional role for 5LO in regulating synaptic plasticity and memory PMID: 23478745
    24. Data indicate that 5-lipoxygenase (5LO) inhibitor zileuton had a significant reduction in Amyloid beta-Protein (Abeta) levels. PMID: 23951061
    25. Inhibitors of the Arachidonate 5-Lipoxygenase pathway do not block P2X7 receptors. PMID: 24743022
    26. 5-LO disruption improves wound healing and alters fibroblast function by an antioxidant mechanism based on HO-1 induction. PMID: 24226420
    27. 5-LO deficiency resulted in reduced mortality due to reduced liver inflammatory and oxidative damage, suggesting 5-LO is a promising target to reduce APAP-induced lethality and liver inflammatory/oxidative damage. PMID: 24288682
    28. LPS-induced Mac-1 expression on monocytes was significantly inhibited by pre-treatment with U-75302, a BLT1-receptor antagonist, suggesting a pivotal role of 5-LO-derived leukotrienes PMID: 23720274
    29. An important role for 5-LO products in sepsis-induced lung injury. PMID: 23947598
    30. High 5-lipoxygenase expression is associated with pulmonary inflammation. PMID: 24076233
    31. These results reveal a fundamental role for 5-lipoxygenase-derived LTB4 in the protective response to Paracoccidioides brasiliensis infection. PMID: 23991239
    32. 5LO plays a functional role in the glucocorticoid-mediated induction of tau pathology. PMID: 23634895
    33. 5-Lipoxygenase facilitates healing after myocardial infarction. PMID: 23812248
    34. 5-LO(-/-) mice exhibited an increased survival rate associated with a decreased number of Paracoccidioides brasiliensis colony forming units. PMID: 23381993
    35. Nonmetastatic cancer cells use metabolites of the 5-LO/leukotriene pathway to induce the generation of immunosuppressive B regulatory cells, thereby promoting cancer escape and metastasis. PMID: 23408836
    36. 5LO-deficient mice have enhanced susceptibility to intrauterine group A Streptococcus (GAS) infection, a major etiologic agent of severe postpartum sepsis. PMID: 23325886
    37. indomethacin-induced hypercontractility observed in Cav-1 knockout tracheas was linked to increased expression of COX-2 and 5-LO, which probably enhanced arachidonic acid shunting and generation of pro-contractile leukotrienes when COX-2 was inhibited. PMID: 22551156
    38. 5-Lipoxygenase mediates RANKL-induced osteoclast formation via the cysteinyl leukotriene receptor 1, and it can serve as a novel therapeutic target for bone-resorption diseases. PMID: 23109727
    39. This study demonistrated that novel functional role that neuronal 5LO plays in exacerbating alzheimer disease-related tau pathologies. PMID: 23034916
    40. 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection. PMID: 22448213
    41. Findings indicate that 5-LO significantly contributes to colonic polyp development and plays an important role in tumorigenesis in colon cancer. PMID: 22072302
    42. deletion of the 5-lipoxygenase activating protein (FLAP) did not influence atherogenesis, it attenuated the proatherogeneic impact of COX-2 deletion in hyperlipidemic mice PMID: 22493243
    43. 5LO plays a role in the development of the anxiety-like phenotype in an age-dependent manner in female mice. PMID: 22220211
    44. a high-fat diet did not change bone formation rates but produced an increase in osteoclast number per bone perimeter in Alox5(-/-) mice PMID: 22128029
    45. The C2-domain is not essential for catalytic activity and does hardly impact reaction specificity. PMID: 21951814
    46. The expression of the Msr1 gene was upregulated in Alox5(-/-) LSCs (leukemia stem cells), suggesting that Msr1 suppresses the proliferation of LSCs. PMID: 21596859
    47. Data from cuprizone-induced model of multiple sclerosis (and rescue with 5-LO inhibitor) suggest that 5-LO contributes to microglial activation and neuroinflammation and to axonal damage resulting in motor dysfunction. PMID: 21555210
    48. Findings suggest that beneficial therapeutic outcomes could be achieved by targeting the 5-LO pathway in the treatment of inflammation associated with hemolytic processes. PMID: 21536805
    49. 5-LO plays a functional role in the glucocorticoid-induced brain Alzheimer Disease-like amyloid pathology PMID: 21253592
    50. Genetic deletion of 5-LO does not prevent development of arthritis in response to infectious agent Borrelia burgdorferi, but rather exacerbates the development of disease and inhibits resolution of the inflammatory response. PMID: 21270404

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  • 亚细胞定位：
    Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Cytoplasm, perinuclear region. Cytoplasm, cytosol. Nucleus envelope. Nucleus intermembrane space.
  • 蛋白家族：
    Lipoxygenase family
  • 组织特异性：
    Expressed in skin Langerhans cells and their emigrated counterparts in draining lymph nodes. Highly expressed in circulating leukocytes.
  • 数据库链接：

    KEGG: mmu:11689

    STRING: 10090.ENSMUSP00000026795

    UniGene: Mm.41072