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  4. Recombinant Mouse Apolipoprotein A-I (Apoa1)

Recombinant Mouse Apolipoprotein A-I (Apoa1)

  • 货号：
    CSB-YP001913MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001913MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001913MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001913MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Apoa1Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I; ProapoA-I); Truncated apolipoprotein A-I]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-264
  • 氨基酸序列
    WHVWQQDEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSSLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTQVQSVIDKASETLTAQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能参考文献：
    1. Altered intestinal lymphatic HDL-ApoA-I and miR-223 metabolism in IR and modulation by niacin may provide insight into the intestinal-mediated regulation of the reverse cholesterol transport pathway PMID: 29183962
    2. We developed a chimaeric molecule termed Fibapo in which FGF19 is covalently coupled to apolipoprotein A-I. Fibapo retains FGF19 biological activities but has significantly increased half-life and hepatotropism. Here we evaluated the pro-regenerative activity of Fibapo in two clinically relevant models where liver regeneration may be impaired: acetaminophen (APAP) poisoning, and PH in aged mice. PMID: 28981086
    3. this study indicates that ionic interactions in the C-terminal domain of apoA-I favor self-association and that monomeric apoA-I is more active in solubilizing phospholipid bilayers. PMID: 29578333
    4. These results suggested that apoA-I overexpression could reduce steatosis by decreasing lipid levels and by suppressing endoplasmic reticulum stress and lipogenesis in hepatocytes. ApoA-I expression could significantly reduce hepatic ER stress and lipogenesis in hepatocytes. PMID: 28577569
    5. ABCA1-derived nascent high-density lipoprotein-apolipoprotein AI and lipids metabolically segregate. PMID: 29074589
    6. apoA-I/ABCA1-mediated cholesterol efflux without STAT3 activation can reduce proinflammatory cytokine expression in macrophages. PMID: 26989082
    7. a novel protective role for ApoA-I in colitis and CAC PMID: 26279300
    8. Our results assign a novel role for 4F(apoA-I mimetic peptide ) as a modulator of the TICE pathway and suggest that the anti-inflammatory functions of 4F may be a partial consequence of the codependent intestinal transport of both 4F and cholesterol. PMID: 27199144
    9. Preincubation of endothelial cells with apoA-I protected against the TNF-alpha-induced inhibition of HTR-8/SVneo (trophoblast) cell integration into endothelial (UtMVEC) networks. These data suggest that a healthy lipid profile may affect pregnancy outcomes by priming endothelial cells in preparation for trophoblast invasion. PMID: 27806983
    10. Reductions in Dio1 expression reduce the expression of ApoA-I in a 3,5,3'-triiodothyronine-/thyroid hormone response element-independent manner. PMID: 27150392
    11. apoA-1 deficiency generates changes in the bone cell precursor population that increase adipoblast, and decrease osteoblast production resulting in reduced bone mass and impaired bone quality PMID: 27088511
    12. This study suggests that enhancement of macrophage cholesterol metabolism by PPARgammais not contributed by activating ABCA1 expression and ABCA1-mediated cholesterol efflux to apoAI, which is not involved by CD36 expression either. PMID: 27890613
    13. Mass spectrometry analysis of peptides derived from chemically crosslinked HDL-SAA particles detected multiple crosslinks between apoA1 and SAA, indicating close proximity (within 25 A) of these two proteins on the HDL surface, providing a molecular and structural mechanism for the simultaneous binding of heparin to apoA1 and SAA. PMID: 27105909
    14. results demonstrate that double deletion of Apoe and Apoa1 ameliorated the amyloid pathology. PMID: 26510953
    15. study suggests that apolipoprotein a1 can alleviate obesity related metabolic disease by inducing AMPK dependent mitochondrial biogenesis. PMID: 25982508
    16. ApoA-I can attenuate lymphocyte activation and autoimmunity in Lupus independently of cholesterol transport, through oxidized fatty acid peroxisome proliferator-activated receptor gamma ligands, and it can reduce renal inflammation in glomerulonephritis. PMID: 26466956
    17. KLF14 regulates plasma HDL-C levels and cholesterol efflux capacity by modulating hepatic ApoA-I production. PMID: 26368306
    18. Akt, through its downstream targets, mTORC1 and hence autophagy, negatively regulates cholesterol efflux to apoA-I. PMID: 25415591
    19. macrophage apoAI expression protects against atherosclerosis and dermatitis by reducing cholesterol accumulation and regulating CD4(+) T-cell levels, without affecting serum HDL or tissue macrophage levels. PMID: 25593328
    20. HDL from apoA1 transgenic mice expressing the 4WF isoform is resistant to oxidative loss of function. PMID: 25561462
    21. ApoA1 levels were not associated with AngII-induced abdominal aortic aneurysms in mice. PMID: 26044581
    22. decreased ApoAI synthesis might be accounted for the lower plasma HDL level in ApoCIII transgenic mice PMID: 25969427
    23. MMP-8-deficient mice had significantly lower serum triglyceride (TG) levels (P = 0.003) and larger HDL particles compared with wild-type (WT) mice. However, no differences were observed in the apoA-I levels. PMID: 25550459
    24. Neutrophil recruitment and the neutrophil cytokines, CXCL1/CXCL2, were suppressed in apo(a)transgenic mice in the abdominal aortic aneurysm model. PMID: 24650562
    25. Expression of apoA-I or ABCA1 can reduce steatosis by decreasing lipid storage in hepatocytes through lipid transport and may also reduce endoplasmic reticulum stress, further lessening hepatic steatosis PMID: 24219083
    26. Data from studies in knockout mice suggest that low apoA1 (and thus low HDL) decreases coenzyme Q10 pool, which in turn decreases electron transfer from electron transport complexes II/III in myocardium mitochondria. PMID: 24759932
    27. role of the hydrophobic residues 225-230 of apoA-I for the biogenesis of HDL PMID: 24123812
    28. the alteration of the hydrophobic 218-222 residues of apoA-I disrupts apoA-I/ABCA1 interactions and promotes the generation of defective pre-beta particles that fail to mature into alpha-HDL subpopulations PMID: 23990662
    29. study identifies a previously unknown proteolytic activity of PLA2 that is specific to apoA-I and may contribute to the enhanced catabolism of apoA-I in inflammation and atherosclerosis. PMID: 24523407
    30. Myeloperoxidase-mediated oxidation renders ApoA-I dysfunctional and unable to promote reverse cholesterol transport, mediate beneficial changes in the composition of atherosclerotic plaques, and pacify the inflammatory status of plaque macrophages. PMID: 24407029
    31. DYRK1A overexpression decreases plasma lecithin:cholesterol acyltransferase activity and apolipoprotein A-I levels. PMID: 23920041
    32. In the C57BL/6 context, but not FVB/N, apoA-I decreases inflammatory macrophage recruitment and monocytosis, contributors to lesion formation PMID: 24334873
    33. ApoA1 enhances resolution of allergen-induced airway inflammation through promoting recovery of damaged TJs in the bronchial epithelium. PMID: 23889245
    34. This study reveals a potent immunomodulatory role for apoA1 in the tumor microenvironment. PMID: 23720750
    35. ApoA-I Helsinki promotes accumulation of ACAT1 in a mouse macrophage cell line. PMID: 23456478
    36. Data indicate that in contrast to apoA-I-knock-out (KO) mice, apoA-I transgenic mice were moderately resistant to cecal ligation and puncture (CLP)-induced septic death. PMID: 23658016
    37. Apo (a) could attenuate the adhesion, migration, and homing abilities of endothelial progenitor cells(EPCs) and could impair the angiogenesis ability of EPCs. PMID: 23581552
    38. Hepatocyte-specific Dyrk1a gene transfer rescues plasma apolipoprotein A-I levels and aortic Akt/GSK3 pathways in hyperhomocysteinemic mice. PMID: 23429073
    39. Genome-wide screen for modulation of hepatic apolipoprotein A-I (ApoA-I) secretion. PMID: 23322769
    40. Mouse apoA-I, which has a relatively polar C-terminal domain, binds to human high-density lipoprotein to approximately half the level of human apoA-I. PMID: 23425306
    41. the involvement of apoA-I in diet-induced The accumulation of triglycerides in hepatocytes and its potential role in the treatment of nonalcoholic fatty liver disease, is reported. PMID: 22576368
    42. Reduced biliary sterol output with no change in total faecal excretion was found in mice expressing a human apolipoprotein A-I variant. PMID: 22845860
    43. A de novo, loss-of-function mutation in the ApoA1 gene of the BcA68 strain prematurely truncates the ApoA1 protein and is associated with a deviant HDLc plasma level. PMID: 22805347
    44. OVA-challenged apoA-I(-/-) mice exhibited a phenotype of increased airway neutrophils compared with WT mice. PMID: 22427535
    45. fed pregnant mice, with or without a deficiency of Mthfr, choline-deficient diets and examined levels of ApoAI, PPARalpha, IFNgamma, and IL-10. ApoAI mRNA was reduced in Mthfr(+/-) and ApoAI protein was reduced due to Mthfr deficiency or choline deficiency. PMID: 22259189
    46. naturally occuring polymorphisms significantly alter the protein self-association properties, the ability of the proteins to clear lipid micelles from solution, and their binding affinity for mature mouse HDL PMID: 22402133
    47. The FGF19 effect on APOA was attenuated by transfection of primary hepatocytes with siRNA against the FGF19 receptor 4 (FGFR4). PMID: 22267484
    48. Data show that cells expressing Cav1 have 2.6-fold more apoA-I binding sites than Cav1(-/-) cells although these additional binding sites are not associated with detergent-free lipid rafts. PMID: 21858084
    49. the role of the PCPE2 protein in an in vivo model PMID: 21771977
    50. analysis of reverse cholesterol transport key players and rescue from global inflammation by ApoA-I(Milano) PMID: 19120689

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 组织特异性：
    Major protein of plasma HDL, also found in chylomicrons.
  • 数据库链接：

    KEGG: mmu:11806

    STRING: 10090.ENSMUSP00000034588

    UniGene: Mm.26743