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  4. Recombinant Mouse Alpha-synuclein (Snca)

Recombinant Mouse Alpha-synuclein (Snca)

In Stock
  • 货号：
    CSB-EP021912MO
  • 规格：
    ￥3324
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Snca; Syn; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    14.5 kDa
  • 表达区域：
    1-140aa
  • 氨基酸序列
    MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
  • 基因功能参考文献：
    1. Taken together, these data suggest that PREP can enhance alpha-synuclein toxicity in vivo. PMID: 29367610
    2. oligodendrocytes but not neurons transform misfolded alpha-Syn into a glial cytoplasmic inclusions-like strain, highlighting the fact that distinct alpha-Syn strains are generated by different intracellular milieus PMID: 29743672
    3. Cardiolipin exposure on the outer mitochondrial membrane modulates alpha-synuclein in Parkinson's disease and cultured cardiomyocyte models. PMID: 29483518
    4. A53T SNCA missense mutation caused impaired light entrainment of the circadian system in mice. PMID: 29865270
    5. Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in alpha-Synuclein Aggregation Disorders PMID: 29759483
    6. In this study, we assessed the spread of pathology following a localized induction of alphaS inclusions in the lumbar spinal cord following a unilateral injection in the sciatic nerve. Using this paradigm, we demonstrated the ability for alphaS inclusion spread and/or induction along neuroanatomical tracts within the CNS of two alphaS-overexpressing mouse models. PMID: 29976670
    7. These results indicated that integrin CD11b mediates alpha-synuclein-induced NOX2 activation through a RhoA-dependent pathway. PMID: 29154191
    8. High SNCA expression is associated with Parkinson's disease. PMID: 28923922
    9. Snca regulates bone network homeostasis and ovariectomy-induced bone loss PMID: 27378017
    10. These findings provide evidence for a novel mechanism underlying the protective effects of PINK1 against alpha-syn-induced neurodegeneration and highlight a novel therapeutic target for Parkinson's disease treatment. PMID: 28933786
    11. study demonstrates that PLK-2 activity can rapidly change cellular alpha-synuclein levels in cell models and in mice brains, but this process does not require phosphorylation of S129. Instead, it operates via regulation of alpha-synuclein mRNA transcription in an open reading frame-dependent manner PMID: 28648742
    12. the presented data link the Parkinson's disease-associated gene alpha-synuclein to the neuronal cell fate determinant TRIM32. PMID: 27339877
    13. alpha-synuclein levels can be reduced in neurons without impairing (or improving) mitochondrial bioenergetics or distribution PMID: 28462393
    14. Study shows that the age-dependent alpha-syn accumulation is correlated with an elevation of TRPC3 in the mitochondrial fractions isolated from monkey and mouse brains. In animal and cell models, alpha-syn overexpression was accompanied by an elevation of alpha-syn and TRPC3 in the mitochondrial fractions, and alpha-syn downregulation was associated with a reduction of the mitochondrial alpha-syn and TRPC3. PMID: 27904950
    15. plasma concentrations of alpha-synuclein were significantly higher in exercising mice compared to control mice. Our results suggest that exercise may slow the progression of Parkinson's disease by preventing abnormal protein aggregation in brain PMID: 29272304
    16. revealed a prominent modulating effect of alpha-synuclein on the developing DA neurons in substantia nigra (SN) which is the most affected region in PD patients. Yet, alpha-synuclein had no effect on the formation of DA neurons in ventral tegmental area which is much less susceptible to degeneration in PD patients. PMID: 29243900
    17. These new mouse lines are invaluable for fast identification of cells with inactivation of Snca by Cre-recombination and represent useful tools for in vivo studies of alpha-synuclein function and dysfunction. PMID: 27838898
    18. Retinal iron dyshomeostasis due to impaired or altered function of alpha-syn contributes to the visual symptoms associated with Parkinson disease. PMID: 27343690
    19. Results obtained with immuno-spin trapping and immunoprecipitation experiments confirmed formation of alpha-synuclein radicals in dopaminergic neurons of maneb and paraquat exposed mice. PMID: 25952542
    20. Study showed that intracerebral injection of synthetic alpha-synuclein fibrils into adult wild-type marmoset brains (caudate nucleus and/or putamen) resulted in spreading of abundant alpha-synuclein pathologies, which were positive for various antibodies to alpha-synuclein, including phospho Ser129-specific antibody, anti-ubiquitin and anti-p62 antibodies, at three months after injection. PMID: 28148299
    21. Trehalose was also able to restore cell viability to control levels, but pre-formed fibrils (PFFs)still exhibited toxic effects on the cells. These data provide essential information regarding effects of trehalose on alphaSyn accumulation and neuronal survival on exposure to PFF PMID: 28068606
    22. These results suggest that synucleins are important orchestrators of presynaptic terminal topography. PMID: 28052246
    23. C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes, have an altered bone phenotype. PMID: 28266709
    24. The findings of this study indicated a functional role of alpha-synuclein in early experimental autoimmune encephalomyelitis by increasing Th1 cell-mediated immune response. PMID: 27565429
    25. synuclein is required for efficient maintenance of animal's balance and coordination in old age. PMID: 27614017
    26. These data show that neurodegenerative processes associated with lysosomal dysfunction may be presynaptically initiated by a concomitant reduction in alpha-synuclein and CSPalpha levels at nerve terminals. PMID: 27881461
    27. synuclein exerts dose-dependent effects on dilation of the exocytotic fusion pore. PMID: 28288128
    28. Data suggest that endocytosis is the principal mechanism by which proteopathic alpha-synuclein aggregates are internalized in primary hippocampal neurons in culture; aggregates are rapidly trafficked along endosomal/lysosomal pathway, where most of the material remains for days as proposed in neurodegenerative synucleinopathies. PMID: 28611062
    29. LRRK2 negatively regulates the clearance of alphaSYN accompanied by down-regulation of the endocytosis pathway; LRRK2 in microglia may function as the offending molecule responsible for neurodegeneration, in terms of down-regulation of alphaSYN clearance. PMID: 27903237
    30. sideroflexin 3 (SFXN3) was found to be a mitochondrial protein localized to the inner mitochondrial membrane. PMID: 28049716
    31. The ability of monomeric alpha-synuclein to enhance ATP synthase efficiency under physiological conditions may be of importance when alpha-synuclein undergoes the misfolding and aggregation PMID: 27733604
    32. Rab7 accumulated in GCase deficient cells, supporting the notion that lysosomal recycling is impaired. Since recombinant GCase can reverse ALR impairment, we anticipate that strategies to restore GCase activity in the brains of both sporadic patients with PD and those with GBA1 mutations will improve autophagy lysosomal pathway, preventing the accumulation of a-synuclein and spread of pathology. PMID: 27378698
    33. Study showed that apoptosis is an important form of cellular degeneration in lipopolysaccharide (LPS-sensitized hypoxic-ischemic (HI) injury in the immature brain. Loss of PINK1 can protect the immature brain against cell apoptosis induced by LPS-sensitized HI injury. Moreover, alpha-Syn plays a neuroprotective role in LPS-sensitized HI brain damage in PINK1-knockout neonatal mice PMID: 27742469
    34. Genetic manipulation of sirtuin 2 levels in vitro and in vivo modulates the levels of alpha-synuclein acetylation, its aggregation, and autophagy. PMID: 28257421
    35. These results indicate that fragmented amyloid-like aggregates of short alpha-syn fibrils are the key pathogenic seeds that trigger prion-like conversion. PMID: 27382062
    36. In summary, we have combined multiple data sets to identify transcripts, which are strong candidates for being phenotypic modifiers, and demonstrated SNCA is a modifier of pathology in motor neuron disease. PMID: 28362802
    37. In alpha-synuclein knock-out mice there was a decrease in neuronal glucose metabolism in cerebral cortex gray matter. PMID: 28039592
    38. alpha-synuclein may have a varying modulating effect on the growth dynamics and the fate of populations of DA neurons. PMID: 27021360
    39. In the olfactory bulb, globus pallidus and substantia nigra pars compacta, alpha-synuclein was expressed, but not in the cerebral cortex, subthalamic nucleus or thalamus. PMID: 26358191
    40. identified alpha-synuclein as a new transcriptional target of p53 PMID: 26833254
    41. High SNCA expression is associated with epilepsy. PMID: 26689812
    42. Data suggest the produced mouse lines represent a set of useful tools for studies of alpha-synuclein normal function and the role of this protein in disease pathogenesis. PMID: 26564109
    43. that loss of glucocerebrosidase function may contribute to SNCA accumulation through inhibition of autophagy via PPP2A inactivation PMID: 26378614
    44. provides evidence for a novel interaction of alpha-synuclein and SOD1 that might be relevant for neurodegenerative diseases PMID: 26643113
    45. It found that chromosome 1 harbors an expression quantitative trait locus that modulates Sncg expression in the mouse retina, and identified the prefoldin-2 (PFDN2) gene as the candidate upstream modulator of Sncg expression. PMID: 26663874
    46. RNA virus-induced Asyn localizes to endoplasmic reticulum-derived membranes, modulates virus-induced endoplasmic reticulum stress signaling, and inhibits viral replication, growth, and injury in the central nervous system. PMID: 26719256
    47. The response of CPLX1 and Foxp1 levels to SNCA deficiency supports the notion that these factors are regulated by altered physiological function of alpha-synuclein. PMID: 25112678
    48. The pattern of expression and distribution of alpha-synuclein during the development of ciliary body and iris of mice, chick and fish data concluded that alpha-synuclein has important cellular function during eye development of studied animals. PMID: 25997379
    49. Longitudinal Metabolomics Profiling of Parkinson's Disease-Related alpha-Synuclein A53T Transgenic Mice. PMID: 26317866
    50. This would suggest that as complex I-deficient cells have already adapted to their mitochondrial defect, the subsequent toxic effects of alpha-synuclein are reduced. PMID: 26181201

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  • 亚细胞定位：
    Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Secreted.
  • 蛋白家族：
    Synuclein family
  • 组织特异性：
    Expressed in brain (at protein level). Highly expressed in presynaptic terminals in the central nervous system.
  • 数据库链接：

    KEGG: mmu:20617

    STRING: 10090.ENSMUSP00000109907

    UniGene: Mm.17484