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  4. Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3)

Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3)

  • 中文名称：
    Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3(Alkbh3),Yeast
  • 货号：
    CSB-YP810366MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3(Alkbh3),Yeast
  • 货号：
    CSB-EP810366MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3(Alkbh3),Yeast
  • 货号：
    CSB-EP810366MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3(Alkbh3),Yeast
  • 货号：
    CSB-BP810366MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3(Alkbh3),Yeast
  • 货号：
    CSB-MP810366MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Alkbh3
  • Uniprot No.：
  • 别名：
    Alkbh3; Abh3Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; EC 1.14.11.54; Alkylated DNA repair protein alkB homolog 3; mAbh3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-286
  • 氨基酸序列
    MEDKRQRARV QGGWATPTKS QSATQPASPA RSRLSQTAGP AWRSKEQQQC DRQFVFKEPQ LVVRAAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDLK EADWILEQLC KDVPWKQRMG IREDVTYPQP RLTAWYGELP YTYSRITMEP NPHWLPVLWT LKSRIEENTS HTFNSLLCNF YRDEKDSVDW HSDDEPSLGS CPVIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD HGTLLIMEGA TQADWQHRVP KEYHSRQPRV NLTFRTVYPD PRGAPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • 基因功能参考文献：
    1. These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
    2. ALKBH2 and ALKBH3 provide cancer protection similar to that of the DNA glycosylase AAG and display apparent epistasis with Aag PMID: 22684101
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    AlkB family
  • 组织特异性：
    Detected in testis, kidney, liver and heart.
  • 数据库链接：

    KEGG: mmu:69113

    STRING: 10090.ENSMUSP00000038721

    UniGene: Mm.272498