Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Actin-related protein 2 (Actr2)

Recombinant Mouse Actin-related protein 2 (Actr2)

  • 货号：
    CSB-YP001248MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001248MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001248MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001248MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001248MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Actr2
  • Uniprot No.：
  • 别名：
    Actr2; Arp2; Actin-related protein 2; Actin-like protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-394
  • 氨基酸序列
    MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI EIKDLMVGDE ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI DTRNCKILLT EPPMNPTKNR EKIVEVMFET YQFSGVYVAI QAVLTLYAQG LLTGVVVDSG DGVTHICPVY EGFSLPHLTR RLDIAGRDIT RYLIKLLLLR GYAFNHSADF ETVRMIKEKL CYVGYNIEQE QKLALETTVL VESYTLPDGR IIKVGGERFE APEALFQPHL INVEGVGVAE LLFNTIQAAD IDTRSEFYKH IVLSGGSTMY PGLPSRLERE LKQLYLERVL KGDVEKLSKF KIRIEDPPRR KHMVFLGGAV LADIMKDKDN FWMTRQEYQE KGVRVLEKLG VTVR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
  • 基因功能参考文献：
    1. We find that the expression of the actin polymerization complex Arp2/3 is reduced in dysbindin-deficient cells, thus affecting actin-dependent phenotypes PMID: 27927957
    2. Arp2 and Arp3 expression was increased under atherosclerotic conditions both in ApoE-/- mice and in oxidized low-density lipoproteins stimulated human coronary artery endothelial cells (HCAECs). PMID: 27181356
    3. Dendritic cells possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. PMID: 26975831
    4. demonstrate that the Arp2/3 complex in higher eukaryotes is actually a family of complexes with different properties PMID: 26655834
    5. Platelet actin nodule formation is dependent on WASp and the ARP2/3 complex. PMID: 26028144
    6. Arp2/3 complex is an essential regulator of adipocyte development through control of the formation of cortical actin structures, which may facilitate nutrient uptake and signalling events. PMID: 25220164
    7. WASH complex regulates Arp2/3 complex and is required for cytokinesis and polar body extrusion. PMID: 24998208
    8. Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation. PMID: 24043783
    9. The Arp2/3 complex may regulate mouse embryo development via its effect on cell division. PMID: 22951093
    10. Cells depleted of Arp2/3 complex cannot respond to a surface-bound gradient of extracellular matrix (haptotaxis). PMID: 22385962
    11. Show that the Arp2/3 complex localizes to the oocyte cortical cap in a Ran-GTPase-dependent manner and nucleates actin filaments in the cortical cap and a cytoplasmic actin network. PMID: 21874009
    12. Scar2 and the Arp2/3 complex appear to be involved in the establishment and maintenance of Golgi polarity during directed migration PMID: 12589062
    13. N-WASP and the Arp2/3 complex trigger actin polymerization during a late step in clathrin-mediated endocytosis PMID: 15085951
    14. Arp2/3 inhibition in growth cones doesn't affect actin organization or inhibit lamellipodia protrusion or filopodia formation. Arp2/3 inhibition enhances axon elongation and causes defects in growth cone guidance. PMID: 15233919
    15. Data show that focal adhesion kinase (FAK) and the Arp2/3 complex associate and colocalize at transient structures formed early after cell adhesion. PMID: 17721515
    16. Arp2-depleted fibroblasts exhibit severe defects in formation of sheet-like protrusions at early time points of cell spreading, with sheet-like protrusions limited to regions along the length of linear protrusions. PMID: 18720401
    17. localises in filopodia and lamellipodia of spreading cells; doesn't depend on local adhesion or on microtubules for localisation PMID: 19068115
    18. WASH complex activates Arp2/3-mediated actin nucleation and binds directly to liposomes. WASH also interacts with dynamin. PMID: 19922875

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection. Nucleus.
  • 蛋白家族：
    Actin family, ARP2 subfamily
  • 数据库链接：

    KEGG: mmu:66713

    STRING: 10090.ENSMUSP00000000137

    UniGene: Mm.259045