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  4. Recombinant Mouse Acid-sensing ion channel 3 (Asic3), partial

Recombinant Mouse Acid-sensing ion channel 3 (Asic3), partial

  • 货号：
    CSB-YP754454MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP754454MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP754454MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP754454MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP754454MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Asic3; Accn3; DrasicAcid-sensing ion channel 3; ASIC3; Amiloride-sensitive cation channel 3; Dorsal root ASIC; DRASIC
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
  • 基因功能参考文献：
    1. ASICs promote the inflammatory response and apoptosis of RAW 264.7 cells. PMID: 30074209
    2. Targeted knockout of Asic3 disrupts spindle afferent sensitivity to dynamic stimuli and impairs mechanotransduction in dorsal root ganglion neurons because of substrate deformation-induced neurite stretching, but not to direct neurite indentation. PMID: 27161260
    3. acid-sensing ion channel 3, activated by concomitant acid and certain pruritogens, constitute a novel signaling pathway that counteracts itch tachyphylaxis to successive pruritogenic stimulation. PMID: 28745101
    4. a small conopeptide of only four amino acids from the venom of Conus textile that strongly potentiated currents of ASIC3, which has a specific role in the pain pathway. This peptide, RPRFamide, belongs to the subgroup of cono-RFamides. PMID: 28396446
    5. High ASIC3 expression is associated with Bone Pain Induced by Multiple Myeloma. PMID: 28254863
    6. This study suggested that the macrophage- and neutrophil-produced IL1beta in the muscles during I/R activates enhanced IL1r1 in muscle afferents increase expression of ASIC3 possibly via JNK activation. PMID: 27358445
    7. segmental analgesia of electroacupuncture-induced analgesia (EA) with lower intensity is partially mediated by ASIC3 receptor on Abeta-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Adelta- and C-fibers PMID: 27896621
    8. the potentiation of ASICs by quinine depends on the presence of the ASIC1a, ASIC2a subunits, but not ASIC1b, ASIC3 subunits. Furthermore, we have determined the amino acids in ASIC1a that are involved in the modulation of ASICs by pHi. PMID: 27402850
    9. fatigue reduces pH in muscle that subsequently activates ASIC3 on macrophages to enhance hyperalgesia to muscle insult. PMID: 25577172
    10. involved in skeletal pain due to high bone turnover PMID: 26340235
    11. ASIC3 is critical for scratching behavior and pathological changes in chronic itch. PMID: 26440887
    12. Data suggest that activation of acid-sensing ion channel 3 (ASIC3) on fibroblast-like synoviocytes (FLS) by acidic pH from an inflamed joint could limit synovial proliferation resulting in reduced inflammatory mediators and subsequent joint damage. PMID: 24923411
    13. we unraveled an important role of ASIC3 in regulating cardiac autonomic function, whereby loss of ASIC3 alters the normal physiological response to ischemic stimuli. PMID: 24804235
    14. The activation of asic3 activity are essential for the development of long-lasting hyperalgesia in acid-induced, chronic, widespread muscle pain. PMID: 24957987
    15. Sea anemone peptide with uncommon beta-hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity. PMID: 23801332
    16. Local pressure induced vasodilation in Asic3+/+ but not Asic3-/- mice, in which high pressure caused ischemia & ulcers. PMID: 22842475
    17. Responses of glomus cells to hypoxia and acidosis are uncoupled, reciprocal and linked to ASIC3 expression PMID: 23165770
    18. Acid-sensing ion channel 3 deficiency increases inflammation but decreases pain behavior in murine arthritis. PMID: 23335302
    19. The results of this study identify ASIC3 channels as an unexpected molecular target for acute actions of 5-HT in inflammatory pain sensation. PMID: 23467344
    20. Currents from ASIC1a(-/-) muscle afferents were less pH-sensitive and displayed faster recovery, currents from ASIC2(-/-) showed diminished potentiation by zinc, and currents from ASIC3(-/-) displayed slower desensitization than those from wild-type. PMID: 23109675
    21. the expression of ASIC3 in dorsal root ganglion (DRG) neurons. PMID: 22070775
    22. Knockdown of ASIC3 in primary afferent fibers innervating muscle prevents the development of inflammatory hyperalgesia in wild-type mice. PMID: 21843914
    23. Data suggest ASIC3 can heteromultimerize with ASIC1a in the brain and alter the biophysical properties of the endogenous channel complex. Also, ASIC subunit composition and channel desensitization may be critical determinants for ASIC-dependent behavior. PMID: 21324060
    24. ASIC3 is a molecular substrate involved in detecting the vessel stretch caused by BVE. PMID: 21304211
    25. ASIC1, ASIC2, and ASIC3 are expressed in dendritic cells (DCs) at the mRNA and protein levels, and extracellular acid can evoke ASIC-like currents in DCs. PMID: 21321108
    26. An isoproterenol-induced cardiac ischemia model mimicking clinical conditions of early cardiac angina was used to demonstrate that ASIC3 but not TRPV1 plays a protective role in sensing cardiac ischemia. PMID: 21127383
    27. In anxiety behavior tasks,Asic3(-/-) mice spent more time in the open arms of an elevated plus maze and displayed less aggressiveness toward intruders but more stereotypic repetitive behaviors during resident-intruder testing than wild-type littermates PMID: 20497234
    28. the proton-induced gastric calcitonin gene-related peptide release in mice lacking the ASIC3 channel was the same as in corresponding wild-type mice PMID: 20100279
    29. Thus, peripherally located ASIC1 and ASIC3 play different roles in the development of hyperalgesia after muscle inflammation. PMID: 20015700
    30. Inadequate response to pups' calls as a result of ASIC3-dependent hearing loss confers maternal deficits in caregivers and social development deficits in their young PMID: 19652708
    31. DRASIC contributes to pH-gated currents in large dorsal root ganglion sensory neurons by forming heteromultimeric channels. PMID: 12037186
    32. ASIC3 is regulated by ASIC2b via the partner protein PICK-1 PMID: 14976185
    33. ASIC3 does not contribute to mechanically activated currents in dorsal root ganglion neurones. PMID: 14990679
    34. Peripheral mechanosensory role for TRPV1 and ASIC3 in the mouse colon that contributes to nociceptive behavior and possibly peripheral sensitization during tissue insult. PMID: 16306411
    35. ASIC1, 2, and 3 are expressed preferentially in colonic neurons within thoracolumbar DRG. QPCR of whole thoracolumbar DRG revealed and abundance of ASIC2 > ASIC1 > ASIC3. PMID: 17177258
    36. Acid sensing ion channel 3 are required for inhibition of visceral nociceptors by benzamil PMID: 17467171
    37. both TRPV1 and ASIC3 may be important peripheral mediators for development of functional (ie, noninflammatory) visceral hypersensitivity PMID: 17553498
    38. proton-sensing GPCRs could have some roles in nociception or in compensation of loss of ASIC3 gene. PMID: 17720533
    39. Gastroesophageal afferents ASIC3 may contribute to acid sensation but plays a more subtle role in responses to distending stimuli. PMID: 17975130
    40. our studies reveal a unique feature of ASIC3-expressing DRG neurons and a basis for their heterogeneous functions PMID: 18082972
    41. ASIC3 and testosterone are necessary to protect against muscle fatigue in a task-dependent manner. ASIC3 expression and the development of exercise-induced fatigue could explain the female predominance in pain syndromes that include muscle fatigue. PMID: 18305024
    42. Decreased pH occurs after inflammation would activate ASIC3 on primary afferent fibers innervating the knee joint, increasing the input to the spinal cord resulting in central sensitization manifested as secondary hyperalgesia of the paw. PMID: 18343037
    43. ASIC3 signaling might be involved in the control of age-dependent glucose intolerance and insulin resistance. PMID: 18466760
    44. Following formalin injection, ASIC3(-/-) mice did not show enhanced pain behaviour PMID: 18801682
    45. provide a novel finding that ASIC3 participates in the maintenance of sub-acute-phase primary hyperalgesia in subcutaneous inflammation and mediates the process of granuloma formation and vasculitis in intramuscular inflammation PMID: 19126241
    46. Data show that ASIC3 immunoreactivity is present in 31% of knee joint afferents and dominantly in small cells. After joint inflammation, ASIC3-immunoreactive neurons significantly increase in number by 50%. PMID: 19185546
    47. ASIC2a and -3 are the major ASIC subunits in cardiac dorsal root ganglia neurons and provide potential molecular targets to attenuate chest pain and deleterious reflexes associated with cardiac disease. PMID: 19590043

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cytoplasm.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, ASIC3 subfamily
  • 组织特异性：
    Expressed in liver, lung, kidney, testis, brain, eye and cochlea. Expressed in spiral ganglion and sensory hair cells of the organ of Corti in the cochlea (at protein level). Expressed in dorsal root ganglion innervating muscles and spinal chord. Expresse
  • 数据库链接：

    KEGG: mmu:171209

    STRING: 10090.ENSMUSP00000039914

    UniGene: Mm.299636