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  4. Recombinant Mouse Achaete-scute homolog 2 (Ascl2)

Recombinant Mouse Achaete-scute homolog 2 (Ascl2)

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  • 中文名称：
    Recombinant Mouse Achaete-scute homolog 2(Ascl2)
  • 货号：
    CSB-EP002200MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Ascl2
  • Uniprot No.：
  • 别名：
    Ascl2; Mash2Achaete-scute homolog 2; ASH-2; mASH-2; mASH2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    32.8 kDa
  • 表达区域：
    1-263aa
  • 氨基酸序列
    MEAHLDWYGVPGLQEASDACPRESCSSALPEAREGANVHFPPHPVPREHFSCAAPELVAGAQGLNASLMDGGALPRLMPTSSGVAGACAARRRQASPELLRCSRRRRSGATEASSSSAAVARRNERERNRVKLVNLGFQALRQHVPHGGANKKLSKVETLRSAVEYIRALQRLLAEHDAVRAALAGGLLTPATPPSDECAQPSASPASASLSCASTSPSPDRLGCSEPTSPRSAYSSEESSCEGELSPMEQELLDFSSWLGGY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This recombinant Mouse Ascl2 protein is typically achieved by the manipulation of Ascl2 gene expression in E.coli cells so that it expresses large amounts of a recombinant Ascl2 gene. In order to get enough amount of the Ascl2 protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Ascl2 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

    Ascl2, a basic helix-loop-helix (bHLH) transcription factor, is required for diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. It is expressed in neuronal precursors. Ascl2 is a downstream target of the Wnt signaling pathway and also a cancer stem cell marker. Ascl2 has been reported to promote cell proliferation and migration in colon cancer and contribute to epithelial-mesenchymal transition (EMT) and promote metastasis in gastric cancer (GC).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
  • 基因功能参考文献：
    1. Ascl2 inhibits myogenic differentiation by targeting MRFs and facilitates the generation of postnatal satellite cells. PMID: 27993983
    2. overexpression of the imprinted Ascl2 gene has considerable consequences for placental development, specifically for the parietal trophoblast giant cell and spongiotrophoblast lineages both of which express pregnancy-related hormones. PMID: 27542691
    3. The ascl2 forms a transcriptional switch that is both Wnt responsive and Wnt dependent to define stem cell identity. PMID: 25620640
    4. Ascl2 directly initiates follicular T-helper cell development PMID: 24463518
    5. TSSC3 plays an important role in the differentiation from trophoblast stem cell to trophoblast progenitors and/or labyrinth trophoblasts through the TSSC3/PI3K/AKT/MASH2 signaling pathway. PMID: 23071113
    6. Elevated expression of Ascl2 in a Wnt signalling dependent manner specifically in the stem cell compartment of the intestine neither increases tumour formation nor diminishes survival in a well established intestinal tumour model, the Apc(min) mouse. PMID: 22138533
    7. Ascl2 knockdown results in tumor growth arrest by miRNA-302b-related inhibition of colon cancer progenitor cells PMID: 22384170
    8. Partial loss of Ascl2 function affects all three layers of the mature placenta and causes intrauterine growth restriction. PMID: 21238448
    9. Mash2 gene is preferentially expressed from the paternal allele. PMID: 7773285
    10. Mash2 is a regulator of Krox24, Mob-1, and CXCR4 expression in cultured Schwann cells and negatively regulates their proliferation; a candidate for missing class B bHLH proteins in peripheral nerves PMID: 12196582
    11. structure of the Mash2 gene and placental Mash2 expression at different gestational ages PMID: 12493693
    12. ascl2 is a putative regulator of proliferation that is overexpressed in intestinal neoplasia PMID: 16568095
    13. The combined results from these gain- and loss-of-function experiments imply that Ascl2 controls intestinal stem cell fate. PMID: 19269367

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:17173

    STRING: 10090.ENSMUSP00000009392

    UniGene: Mm.196417