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  4. Recombinant Mouse APOBEC1 complementation factor (A1cf)

Recombinant Mouse APOBEC1 complementation factor (A1cf)

  • 货号：
    CSB-YP001002MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001002MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001002MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001002MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001002MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    A1cf
  • Uniprot No.：
  • 别名：
    A1cf; Acf; AspAPOBEC1 complementation factor; APOBEC1-stimulating protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-595
  • 氨基酸序列
    MESNHKSGDG LSGTQKEAAL RALVQRTGYS LVQENGQRKY GGPPPGWDST PPERGCEIFI GKLPRDLFED ELIPLCEKIG KIYEMRLMMD FNGNNRGYAF VTFSNKQEAK NAIKQLNNYE IRTGRLLGVC ASVDNCRLFV GGIPKTKKRE EILSEMKKVT EGVVDVIVYP SAADKTKNRG FAFVEYESHR AAAMARRRLL PGRIQLWGHP IAVDWAEPEV EVDEDTMSSV KILYVRNLML STSEEMIEKE FNSIKPGAVE RVKKIRDYAF VHFSNREDAV EAMKALNGKV LDGSPIEVTL AKPVDKDSYV RYTRGTGGRN TMLQGEYTYP LSHVYDPTTT YLGAPVFYTP QAYAAIPSLH FPATKGHLSN RALIRTPSVR EIYMNVPVGA AGVRGLGGRG YLAYTGLGRG YHVKGDKRED KLYDLLPGME LTPMNTVSLK PQGIKLAPQI LEEICQKNNW GQPVYQLHSA IGQDQRQLFL YKVTIPALAS QNPAIHPFIP PKLSAYVDEA KRYAAEHTLQ TLGIPTEGGD AGTTAPTATS ATVFPGYAVP SATAPVSTAQ LKQAVTLGQD LAAYTTYEVY PTFALTTRGD AYGTF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay.
  • 基因功能参考文献：
    1. Here, we show that partial loss of either APOBEC1 complementation factor (A1CF), the RNA-binding cofactor of APOBEC1 in RNA editing, or Argonaute 2 (AGO2), a key factor in the biogenesis of certain noncoding RNAs, modulates risk for TGCTs and testicular abnormalities in both parent-of-origin and conventional genetic manners. PMID: 27582469
    2. The observations demonstrate that A1CF does not act as the APOBEC1 complementation factor in vivo under normal physiological conditions and suggest new roles for A1CF, specifically within the male adult kidney. PMID: 28069890
    3. Data show that the importance of ACF for modulating ApoB was supported by the finding that RNAi knockdown of ACF reduced ApoB secretion. PMID: 20541607
    4. ACF regulates liver regeneration following partial hepatectomy at least in part by controlling the stability of IL-6 mRNA PMID: 20406809
    5. ACF plays a crucial role, which is independent of apobec-1 expression, in cell survival, particularly during early embryonic development PMID: 16055734

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  • 亚细胞定位：
    Nucleus. Endoplasmic reticulum. Cytoplasm.
  • 组织特异性：
    Expressed primarily in liver, small intestine and kidney.
  • 数据库链接：

    KEGG: mmu:69865

    STRING: 10090.ENSMUSP00000075235

    UniGene: Mm.87538