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  4. Recombinant Mouse 14-3-3 protein sigma (Sfn)

Recombinant Mouse 14-3-3 protein sigma (Sfn)

  • 货号：
    CSB-YP021135MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021135MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021135MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021135MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021135MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sfn; Mkrn314-3-3 protein sigma; Stratifin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-248
  • 氨基酸序列
    MERASLIQKA KLAEQAERYE DMAAFMKSAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VKEYREKVET ELRGVCDTVL GLLDSHLIKG AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADSAGEEGG EAPEEPQS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
  • 基因功能参考文献：
    1. Loss of 14-3-3-sigma sensitizes mice to chemically-induced skin carcinogenesis. Therefore, 14-3-3-sigma may indeed represent a mediator of tumor suppression in the skin. PMID: 27409835
    2. 14-3-3sigma stabilizes a complex of soluble actin and intermediate filament to enable breast tumor invasion. PMID: 24067649
    3. these data provides the first evidence that 14-3-3 sigma is a Smad3-dependent target gene of TGF-beta1. PMID: 23375543
    4. 14-3-3sigma plays an important role in regulating mouse embryonic stem cell proliferation by binding and sequestering phosphorylated GSK-3beta and enhancing Wnt-signaled GSK-3beta inactivation. PMID: 22768254
    5. We show that mice heterozygous for the 14-3-3sigma mutation have severe defects in hair shaft differentiation, resulting in destruction of the hair shaft during morphogenesis. PMID: 22377760
    6. Data show that endogenous 14-3-3sigma protein formed a complex with FOXO1 protein. PMID: 21205887
    7. 14-3-3 sigma is required for TGF-beta1-mediated growth inhibition in mouse mammary epithelial cells. PMID: 20082218
    8. 14-3-3 sigma is needed for normal hair growth PMID: 20237493
    9. 14-3-3sigma plays a critical role in retaining epithelial polarity PMID: 20439433
    10. 14-3-3sigma is critical for regulating corneal epithelial proliferation and differentiation by regulating Notch signaling activity. PMID: 20100467
    11. 14-3-3 can mediate the relocalization of nuclear ligands by several mechanisms that ensure complete sequestration of the bound 14-3-3 complex in the cytoplasm. PMID: 11864996
    12. Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth. PMID: 12075357
    13. Keratin 10 -/- mice exhibited induction of c-Myc, cyclin D1, 14-3-3sigma and wound healing keratins K6 and K16. PMID: 12077355
    14. 14-3-3 sigma appears to have a positive feedback effect on p53 activity PMID: 14517281
    15. P4 regulates the phosphorylation status of 14-3-3sigma through a PKG-dependent pathway, and 14-3-3sigma plays a central and essential role in maintaining the viability of spontaneously immortalized granulosa cell. PMID: 15286034
    16. mutation in stratifin is responsible for the repeated epilation phenotype PMID: 16200063
    17. 14-3-3sigma is a crucial regulator for skin proliferation and differentiation. A single nucleotide insertion in the Sfn coding region results in a truncated protein linked with phenotypes of Er-heterozygous and -homozygous mice. PMID: 16239341
    18. 14-3-3sigma stabilizes p27 Kip1 by inhibiting the activity of PKB/Akt PMID: 16532026
    19. IKKalpha protects the 14-3-3sigma locus from hypermethylation, which serves as a mechanism of maintaining genomic stability in keratinocytes. PMID: 17643371
    20. These observations suggest that, in addition to an increased gene copy number and upregulation of EGR2 and CITED1, an elevated erbB2 transcript level involves the loss of 14-3-3sigma, which sequesters a key transcriptional regulator of the erbB2 promoter. PMID: 17938205

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 蛋白家族：
    14-3-3 family
  • 组织特异性：
    Expressed in the basal layer of skin epithelium and in outer root sheath of hair follicle.
  • 数据库链接：

    KEGG: mmu:55948

    STRING: 10090.ENSMUSP00000050374

    UniGene: Mm.44482