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  4. Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (Abhd5)

Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (Abhd5)

  • 中文名称：
    Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(Abhd5),Yeast
  • 货号：
    CSB-YP875273MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(Abhd5),Yeast
  • 货号：
    CSB-EP875273MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(Abhd5),Yeast
  • 货号：
    CSB-EP875273MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(Abhd5),Yeast
  • 货号：
    CSB-BP875273MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(Abhd5),Yeast
  • 货号：
    CSB-MP875273MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Abhd5
  • Uniprot No.：
  • 别名：
    Abhd51-acylglycerol-3-phosphate O-acyltransferase ABHD5; EC 2.3.1.51; Abhydrolase domain-containing protein 5; Lipid droplet-binding protein CGI-58; Protein CGI-58
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-351
  • 氨基酸序列
    MKAMAAEEEV DSADAGGGSG WLTGWLPTWC PTSTSHLKEA EEKMLKCVPC TYKKEPVRIS NGNRIWTLMF SHNISSKTPL VLLHGFGGGL GLWALNFEDL STDRPVYAFD LLGFGRSSRP RFDSDAEEVE NQFVESIEEW RCALRLDKMI LLGHNLGGFL AAAYSLKYPS RVSHLILVEP WGFPERPDLA DQERPIPVWI RALGAALTPF NPLAGLRIAG PFGLSLVQRL RPDFKRKYSS MFEDDTVTEY IYHCNVQTPS GETAFKNMTI PYGWAKRPML QRIGGLHPDI PVSVIFGARS CIDGNSGTSI QSLRPKSYVK TIAILGAGHY VYADQPEEFN QKVKEICHTV D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors. Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion.
  • 基因功能参考文献：
    1. suppresses spermidine synthase-dependent spermidine production in tumour-associated macrophages and potentiates the growth of colorectal cancer PMID: 27189574
    2. Mice lacking brown adipose tissue CGI-58 gene, a lipolytic activator essential for the stimulated lipid droplet lipolysis, have normal thermogenic capacity and are not cold sensitive. PMID: 28988822
    3. Prebinding CGI-58 with PI(3)P or PI(5)P did not alter its coactivation of ATGL in vitro. In summary, purified recombinant CGI-58 that is functional as an ATGL coactivator lacks LPAAT activity. PMID: 24879803
    4. ABHD5 possesses a PNPLA2-independent function in regulating autophagy and tumorigenesis. PMID: 27559856
    5. omega-O-AcylCer and CLE formation critically depend on CGI-58 expression in differentiated keratinocytes. PMID: 27725204
    6. These results demonstrate that macrophage CGI-58 enhances PPAR-gamma signaling and thus suppresses inflammatory responsiveness and mitochondrial dysfunction in macrophages. PMID: 26872126
    7. we identify adipocyte-type fatty acid-binding protein (A-Fabp) and other members of the fatty acid-binding protein (Fabp) family as interaction partners of Cgi-58. PMID: 25953897
    8. Data (including data from studies in atherosclerotic knockout mice) suggest CGI58-deficient macrophages store-up triglyceride-rich droplets and have reduced phagocytic capacity, comparable to Atgl- (adipose triglyceride lipase-)deficient macrophages. PMID: 25316883
    9. -mediated phosphorylation of CGI-58 is required for dispersion of CGI-58 from perilipin 1A-coated lipid droplets PMID: 25421061
    10. Muscle CGI-58 deficiency causes cardiac dysfunction and fat deposition in oxidative muscles but induces a series of favorable metabolic changes in mice fed a high-fat diet. PMID: 25751639
    11. intestinal CGI-58 is required for efficient postprandial lipoprotein-TG secretion and for maintaining hepatic and plasma lipid homeostasis. PMID: 24618586
    12. CGI-58 transgenic/ApoE-/- mice exhibited an anti-atherosclerosis phenotype compared with wild type (WT) controls (CGI-58 WT/ApoE-/-), illustrated by less plaque area in aortic roots. PMID: 25178844
    13. Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis. PMID: 24927580
    14. The CGI-58 deficiency in the liver directly causes not only hepatic steatosis but also steatohepatitis and fibrosis. PMID: 23733885
    15. Data indicate that CGI-58 knockdown leads to increased diacylglycerol (DAG) acumulation in the lipid droplet/ER, preventing DAG accumulation and PKCepsilon translocation to the plasma membrane. PMID: 23302688
    16. an important metabolic function of CGI-58 in skeletal muscle PMID: 22383684
    17. show that CGI-58 is required for the generation of signaling lipids in response to inflammatory stimuli and that lipid second messengers generated by CGI-58 play a critical role in maintaining the balance between inflammation and insulin action PMID: 22228714
    18. minimal fragment of ATGL could still be activated and inhibited by CGI-58 and G0S2, respectively PMID: 22039468
    19. Overexpression of ABHD5 did not prevent the development of obesity in mice fed a high-fat diet PMID: 21885429
    20. CGI-58 plays a critical role in limiting hepatic steatosis and maintaining hepatic glycerophospholipid homeostasis and in promoting high-fat-diet-induced obesity and insulin resistance. PMID: 20802159
    21. findings show CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids PMID: 19801371
    22. Data report a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S, that has a predominant cytoplasmic location. The splicing event may be involved in the regulation of lipid homeostasis. PMID: 20083112
    23. CGI-58 interacts with perilipin and is localized to lipid droplets PMID: 15136565
    24. CGI-58 binds to perilipin A-coated lipid droplets in a manner that is dependent upon the metabolic status of the adipocyte and the activity of cAMP-dependent protein kinase PMID: 15292255
    25. CGI-58 facilitates lipolysis in cooperation with perilipin and other factors, including lipases PMID: 17308334
    26. CGI-58, the causative gene for Chanarin-Dorfman syndrome, mediates acylation of lysophosphatidic acid PMID: 18606822
    27. CGI-58 is expressed and packaged into lamellar granules (LG) during keratinization and likely plays crucial role(s) in keratinocyte differentiation and LG lipid metabolism, contributing to skin lipid barrier formation. PMID: 18832586

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  • 亚细胞定位：
    Cytoplasm. Lipid droplet. Cytoplasm, cytosol. Note=Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • 蛋白家族：
    Peptidase S33 family, ABHD4/ABHD5 subfamily
  • 组织特异性：
    Highly expressed in the adipose tissue and testes. Weakly expressed in the liver, muscle, kidney, and heart. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and hypothalamus in brain (at protein level).
  • 数据库链接：

    KEGG: mmu:67469

    STRING: 10090.ENSMUSP00000122274

    UniGene: Mm.280254