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  4. Recombinant Methanocaldococcus jannaschii Isopropylmalate/citramalate isomerase large subunit (leuC)

Recombinant Methanocaldococcus jannaschii Isopropylmalate/citramalate isomerase large subunit (leuC)

  • 货号：
    CSB-YP305647MRU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP305647MRU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP305647MRU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP305647MRU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP305647MRU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    leuC
  • Uniprot No.：
  • 别名：
    leuC; MJ0499Isopropylmalate/citramalate isomerase large subunit; EC 4.2.1.33; EC 4.2.1.35,; R)-2-methylmalate dehydratase,; R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease; EC 4.2.1.31
  • 种属：
    Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-424
  • 氨基酸序列
    MGMTIVEKIL AKASGKKEVS PGDIVMANID VAMVHDITGP LTVNTLKEYG IEKVWNPEKI VILFDHQVPA DSIKAAENHI LMRKFVKEQG IKYFYDIREG VCHQVLPEKG HVAPGEVVVG ADSHTCTHGA FGAFATGIGS TDMAHVFATG KLWFKVPETI YFNITGDLQP YVTSKDVILS IIGEVGVDGA TYKACQFGGE TVKKMSIASR MTMTNMAIEM GGKTGIIEPD EKTIQYVKEA MKKHGTERPF EVIKGDEDAE FAEVYEIEAD KIEPVFACPH NVDNVKQARE VAGKPIDQVF IGSCTNGRLE DLRMAIKIIE KHGGIADDVR VVVTPASREE YLKALKEGII EKFLKYGCVV TNPSCSACMG SLYGVLGPGE VCVSTSNRNF RGRQGSLEAE IYLASPITAA ACAVKGELVD PRDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate, a step involved in leucine biosynthesis. Catalyzes the isomerization between 2-methylmalate and 3-methylmalate, via the formation of 2-methylmaleate (citraconate), a step involved in isoleucine biosynthesis. Also displays malease activity, i.e. catalyzes the hydration of maleate to form (R)-malate.
  • 基因功能参考文献：
    1. first structures of oxidized and reduced forms of the large subunit of IPM isomerase (ox-MJ0499 and red-MJ0499, respectively) from M. jannaschii are reported here at 1.8 and 2.7 A resolution, respectively PMID: 24699638
  • 蛋白家族：
    Aconitase/IPM isomerase family, LeuC type 2 subfamily
  • 数据库链接：

    KEGG: mja:MJ_0499

    STRING: 243232.MJ_0499