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  4. Recombinant Macaca fascicularis albumin (ALB)

Recombinant Macaca fascicularis albumin (ALB)

In Stock
  • 货号：
    CSB-MP001561MOV
  • 规格：
    ￥2640
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    ALB; Qlv-U347A-C12; Qlv-U377A-G8Serum albumin
  • 种属：
    Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Mammalian cell
  • 分子量：
    76.6kDa
  • 表达区域：
    25-608aa
  • 氨基酸序列
    DTHKSEVAHRFKDLGEEHFKGLVLVAFSQYLQQCPFEEHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPPLVRPEVDVMCTAFHDNEATFLKKYLYEVARRHPYFYAPELLFFAARYKAAFAECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGDRAFKAWAVARLSQKFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYMCENQDSISSKLKECCDKPLLEKSHCLAEVENDEMPADLPSLAADYVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVMLLLRLAKAYEATLEKCCAAADPHECYAKVFDEFQPLVEEPQNLVKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGAKCCKLPEAKRMPCAEDYLSVVLNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALELDEAYVPKAFNAETFTFHADMCTLSEKEKQVKKQTALVELVKHKPKATKEQLKGVMDNFAAFVEKCCKADDKEACFAEEGPKFVAASQAALA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

First, it must be expressed in Yeast and a His tag.
Second, they need endotoxin removal and a guarantee of less than 0.5 EU/mL.
Finally, we would like to know what purification and QC methods would be used to test and verify all parameters.

A:
Thanks for your inquiry
1. This is a protein we successfully developed before. The current protein is with N-terminal 6xHis-tagged and expressed in yeast system which can satisfy your requirement.
2. We could perform Endotoxin Removal free of charge and guarantee endotoxin level <1.0 EU/ug of the protein by the LAL method.
This endotoxin removal is semi-quantitative, we can guarantee that the endotoxin level is less than 1.0 EU/ug, but we cannot determine a specific value.
Endotoxin (LPS) is mainly present in Gram-negative bacteria, which is the main constituent of the outer membrane of the cell wall, so it is mainly targeted for E. coli recombinant expression system.
Theoretically, there should exist no endotoxin in other recombinant expression systems.
However, laboratory reagents (including deionized water) and instruments may be affected by some endotoxin. The endotoxin content produced by this reason should be not too high in itself,
and it may be able to meet the needs of the customer, but we cannot guarantee it.
3. This protein has a his tag. The separation and purification of our protein is performed by Ni column affinity chromatography. The size of the band is detected by SDS-PAGE.
1) During the production process, we will perform sequencing in the cloning stage to ensure that the sequence is 100% correct (we can also provide sequencing reports if necessary).
2) After protein expression, SDS-PAGE is used to detect the band size, which is in line with the theoretical value.
3) We have a comprehensive purity of 90% or more by SDS-PAGE Coomassie blue staining and Bandscan software analysis.
4) You can perform SDS-PAGE test, and if available, mass spectrometry.

靶点详情

  • 功能：
    Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma.
  • 数据库链接：

    KEGG: mcf:102130757

    UniGene: Mfa.4123