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  4. Recombinant Kluyveromyces lactis ATPase GET3 (GET3)

Recombinant Kluyveromyces lactis ATPase GET3 (GET3)

  • 货号：
    CSB-YP728343KBK
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP728343KBK
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP728343KBK-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP728343KBK
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP728343KBK
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GET3
  • Uniprot No.：
  • 别名：
    GET3; KLLA0E01497gATPase GET3; EC 3.6.-.-; Arsenical pump-driving ATPase; Arsenite-stimulated ATPase; Golgi to ER traffic protein 3; Guided entry of tail-anchored proteins 3
  • 种属：
    Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-349
  • 氨基酸序列
    MDLTVEPDLN SLITSSTHRW IFVGGKGGVG KTTSSCSIAI QMALAQPEKQ YLLISTDPAH NLSDAFGEKF GKDARKVTGM NNLSCMEIDP SAALKDMNDM AVANNATGSG EFSDLLQGGA LSELTGSIPG IDEALSFMEV MKHIKNQEQG EGDRYDTVIF DTAPTGHTLR FLQLPSTLSK LLEKFGEITA RLGPMLNSLA GANNVDLVGK MSELKSNVEK IKEQFTNPDM TTFVCVCISE FLSLYETERL VQELISYDMD VNSIIVNQLL FAEYDEGDSC KRCQSRWKMQ KKYLDQIDEL YEDFHIVKMP LCAGEIRGLN NLKKFSQFLR KPYDPVADSK VIYELEQQD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.
  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum. Golgi apparatus.
  • 蛋白家族：
    ArsA ATPase family
  • 数据库链接：

    KEGG: kla:KLLA0E01497g

    STRING: 284590.XP_454016.1