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  4. Recombinant Human coronavirus 229E Nucleoprotein (N)

Recombinant Human coronavirus 229E Nucleoprotein (N)

In Stock
  • 货号：
    CSB-EP322753HIT
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nucleocapsid protein
  • 种属：
    Human coronavirus 229E (HCoV-229E)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    49.4 kDa
  • 表达区域：
    1-389aa
  • 氨基酸序列
    MATVKWADASEPQRGRQGRIPYSLYSPLLVDSEQPWKVIPRNLVPINKKDKNKLIGYWNVQKRFRTRKGKRVDLSPKLHFYYLGTGPHKDAKFRERVEGVVWVAVDGAKTEPTGYGVRRKNSEPEIPHFNQKLPNGVTVVEEPDSRAPSRSQSRSQSRGRGESKPQSRNPSSDRNHNSQDDIMKAVAAALKSLGFDKPQEKDKKSAKTGTPKPSRNQSPASSQTSAKSLARSQSSETKEQKHEMQKPRWKRQPNDDVTSNVTQCFGPRDLDHNFGSAGVVANGVKAKGYPQFAELVPSTAAMLFDSHIVSKESGNTVVLTFTTRVTVPKDHPHLGKFLEELNAFTREMQQHPLLNPSALEFNPSQTSPATAEPVRDEVSIETDIIDEVN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The DNA fragment encoding the 1-389aa of the humancoronavirus 229E Nucleoprotein (N) protein was fused with N-terminal 6xHis tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant human coronavirus 229E Nucleoprotein (N) protein. The purity of this recombinant Nucleoprotein (N) protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This Nucleoprotein (N) protein showed a band on the gel with a molecular weight of approximately 55 kDa.

    Studies indicated that N protein could interact with SARS and COVID-19. Among them, a study pointed that in patients with COVID-19, N antibodies have been observed, suggesting that N protein is capable of producing an immune response. To some extent, researchers revealed that N protein is very likely to preform additional roles during the viral infection. Besides, N proteins are served as inhibitors of interferon (IFN) in pathogenicity because N proteins were documented to antagonize the IFN response. Some researchers also discovered that N proteins of SARS-CoV-2 plays an important role in the inhibition of IFN-βproduction.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
  • 基因功能参考文献：
    1. N protein mutants with truncated C-terminal domains were generated and investigated by biophysical and biochemical analyses to clarify the role of the C-terminal tail of the N protein in oligomerization PMID: 23178926
    2. Nucleocapsid protein expression facilitates coronavirus replication PMID: 17037502
  • 亚细胞定位：
    Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.
  • 蛋白家族：
    Alphacoronavirus nucleocapsid protein family
  • 数据库链接：

    KEGG: vg:918763