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  4. Recombinant Human cGMP-dependent protein kinase 1 (PRKG1)

Recombinant Human cGMP-dependent protein kinase 1 (PRKG1)

  • 货号：
    CSB-YP619797HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP619797HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP619797HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP619797HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP619797HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    cGK 1; cGK1; cGKI alpha; cGKI; cGKI beta; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; DKFZp686K042; FLJ36117; KGP1_HUMAN; KGPB; MGC71944; pkg; PRKG1; PRKG1B; PRKGR1A; PRKGR1B
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-671
  • 氨基酸序列
    SELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPEEIL SKLADVLEET HYENGEYIIR QGARGDTFFI ISKGTVNVTR EDSPSEDPVF LRTLGKGDWF GEKALQGEDV RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD SFPEDNDEPP PDDNSGWDID F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates IRAG1 and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.
  • 基因功能参考文献：
    1. our study provides the first genome-wide evidence regarding a mechanism by which traumatic life events interact with human genetic variation of PRKG1 in relation to alcohol misuse PMID: 28265120
    2. Disulfide-activated PKGIalpha regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. PMID: 27782102
    3. PRKG1, known to induce apoptosis when activated, is increased in all patient derived glioblastoma spheres PMID: 27564115
    4. redox-mediated guidance required PKG1 and consequently, its deficiency in vivo resulted in defective repair and enhanced neuropathic pain after nerve injury. PKG1-dependent repair functions will outweigh its signaling functions in spinal nociceptive LTP, so that inhibition of PKG1 is no option for neuropathic pain. PMID: 27978504
    5. Oxidant signaling underlies PKGIalpha modulation of Ca2+ sparks and BKCa in myogenically active arterioles PMID: 27729549
    6. These affinity and activation measurements show that beta-phenyl-1,N2-ethenoguanosine 3',5'-monophosphate (PET-cGMP) is most selective for PKG I, whereas 8-(4-chlorophenylthio)guanosine-3'-5'-cyclic monophosphate (8-pCPT-cGMP) is most selective for PKG II. PMID: 28793191
    7. Hence, SO2 exerted its vasodilatory effects at least partly by promoting disulfide-dependent dimerization of sGC and PKG, resulting in an activated sGC/cGMP/PKG pathway in blood vessels. These findings revealed a new mode of action and mechanisms by which SO2 regulated the vascular tone. PMID: 27009048
    8. these data indicate PKG as an intermediary in EGFR-mediated cell death, likely via apoptotic pathway. PMID: 27381222
    9. The heterozygous c.530G>A:p.Arg177Gln PRKG1 variant was associated with early-onset thoracic aortic aneurysm and dissection in a Spanish family. PMID: 27442293
    10. cAMP is a partial agonist for PKG, and we elucidate the mechanism for cAMP partial agonism through the comparative NMR analysis of the apo, cGMP-, and cAMP-bound forms of the PKG cyclic nucleotide-binding domain B. PMID: 26370085
    11. Protein kinase G has a role in regulating production of H2S, which governs oxygen sensing PMID: 25900831
    12. The C42-C42' disulfide bond dramatically stabilizes PRKG1. PMID: 26132214
    13. The Golgi apparatus regulates PKGIalpha compartmentation and proteolysis. PMID: 25855081
    14. Study of genetic heterogeneity in systemic lupus erythematosus, the top associations in European ancestry were protein kinase, cyclic GMP-dependent, type I (PRKG1) rs7897633 (P(Meta) = 2.75 x 10(-8)) and purine nucleoside phosphorylase (PNP) rs1049564 (P(Meta) = 1.24 x 10(-7)). PMID: 25338677
    15. Expression levels of miR-517a-3p and its target mRNA PRKG1 were inversely correlated in NK cells before and after obstetric delivery. PMID: 25273530
    16. miR-20a expression is up-regulated in response to hypoxia in both mouse and human pulmonary arterial smooth muscle cells PMID: 25447536
    17. Data show that functional cGMP/protein kinase G type 1 (PKG1) is expressed in pancreatic adenocarcinoma cells. PMID: 24826884
    18. These findings characterize PKG as a novel regulator of AR-mediated transcription by enhancing AR cofactor p44/WDR77's function. PMID: 23755100
    19. Neutron diffraction reveals hydrogen bonds critical for cGMP-selective activation: insights for cGMP-dependent protein kinase agonist design. PMID: 25271401
    20. Leucine296 and Arginine297 are key residues of PKG I that mediate cGMP selectivity. PMID: 24239458
    21. These data suggest that increased PKG II activity attenuates bFGFinduced proliferation and migration by inhibiting the MAPK/ERK signaling pathway, whereas PKG I does not PMID: 23404188
    22. nitration of Tyr(247) and the attenuation of cGMP binding is an important mechanism regulating in PKG-1alpha activity and SMC proliferation/differentiation PMID: 24469460
    23. s show for the first time that PKG is also able to stably interact with a viral substrate, West Nile virus NS5, in cell culture and in vitro. PMID: 23876037
    24. These findings indicate that PRKG1 risk alleles associate with salt-sensitivity related to a loss of the inhibitory control of renal Na(+) reabsorption, suggestive of a blunt pressure-natriuresis response. PMID: 24060892
    25. left ventricular systolic radial function was associated with common polymorphisms in PRKG1. PMID: 24060898
    26. Recurrent gain-of-function mutation in PRKG1 causes thoracic aortic aneurysms and acute aortic dissections. PMID: 23910461
    27. activation of PKGI with cGMP regulated SSG1 intracellular distribution. PMID: 23831687
    28. ANP attenuates the inflammatory actions of histamine via endothelial GC-A/cGMP/cGKI signaling and inhibitory phosphorylation of TRPC6 channels. PMID: 23814119
    29. The data suggest that PKGIbeta enhances breast cancer cell motility and invasive capacity, at least in part, by phosphorylating CaD. PMID: 23418348
    30. PKG positively regulates proteasome activities and proteasome-mediated degradation of misfolded proteins, likely through posttranslational modifications to proteasome subunits. PMID: 23770744
    31. overexpression of PCs, furin and PC5, but not PC7, which are all expressed in SMC, increase PKGI cleavage in a dose-dependent manner PMID: 23686857
    32. The cyclic GMP-dependent kinase Ialpha -induced RhoA activation is dependent on the activity of ROCK and serine/threonine phosphatases. PMID: 23195829
    33. Studies indicate the presence of the switch helix (SW), a novel motif that promotes the formation of a domain-swapped dimer in the asymmetric unit. PMID: 23416533
    34. Low myocardial PKG activity in heart failure with preserved ejection fraction was associated with raised cardiomyocyte F(passive) and was related to increased myocardial nitrosative/oxidative stress. PMID: 22806632
    35. RhoA is a PKGIalpha target and direct binding of activated PKGIalpha to RhoA is central to cGMP-mediated inhibition of the VSMC Rho kinase contractile pathway PMID: 23066013
    36. Downregulation of PKG1 was associated with malignant and benign breast tumors. PMID: 22791569
    37. These results suggest that DAPK2 is one of the targets of cGK-I in apoptosis induction. PMID: 22580283
    38. PKGIbeta inhibited cell growth and inducd apoptosis in breast cancer cells. PMID: 22095901
    39. cGMP-dependent protein kinase (PKG) Ialpha transfection significantly attenuated the hypoxia-induced down-regulation of the expressions of SM-alpha-actin, MHC and calponin. PMID: 22293199
    40. found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
    41. Cyclic 3',5'-guanosine monophosphate-dependent protein kinase inhibits colon cancer cell adaptation to hypoxia. PMID: 21563174
    42. cGMP dependent kinase 1 alpha interacts with and phosphorylates transient receptor potential cation channel subfamily C member 7 PMID: 21402151
    43. data suggest that activation of PKG signaling in pericytes inhibits focal adhesion formation by inhibiting Rac1 PMID: 21451103
    44. NO, depending on its concentration, may act in human PBMCs as a stimulator of IL-6 expression and modulator of NF-KB involving the sGC/cGMP/PKG pathway PMID: 21414799
    45. The mechanism of cyclic nucleotide binding to PKG was investigated by determining crystal structures of the amino-terminal cyclic nucleotide-binding domain (CNBD-A) of human PKG I bound to either cGMP or cAMP. PMID: 21526164
    46. The novel cGMP/PKG/ROS/calmodulin/CaMKII signaling pathway may regulate cardiomyocyte excitability by opening K(ATP) channels and contribute to cardiac protection against ischemia-reperfusion injury. PMID: 21479273
    47. These findings provide evidence of a physical and compartmentalized association between SERT and PKGIalpha that supports. PMID: 19656393
    48. A novel mechanism has been elucidated that is able to increase PKG-I protein content in contractile cells, with hypotensive consequences in rat thoracic aorta. PMID: 21160032
    49. disruption of the IP3R1-IRAG calcium regulation system is a novel cause of dysfunctional osteoclasts unrelated to defects in attachment proteins or acid secretion PMID: 20567233
    50. structure provides molecular details of this unique leucine/isoleucine zipper, revealing specific hydrophobic and ionic interactions that mediate dimerization and demonstrating the topology of the GKAP interaction surface. PMID: 20826808

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  • 相关疾病：
    Aortic aneurysm, familial thoracic 8 (AAT8)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily
  • 组织特异性：
    Primarily expressed in lung and placenta.
  • 数据库链接：

    HGNC: 9414

    OMIM: 176894

    KEGG: hsa:5592

    UniGene: Hs.407535