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  4. Recombinant Human Zinc finger protein GLI2 (GLI2), partial

Recombinant Human Zinc finger protein GLI2 (GLI2), partial

  • 货号：
    CSB-EP009500HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    CJS; Gli 2; GLI family zinc finger 2; GLI Kruppel family member GLI2; GLI2; GLI2_HUMAN; Glioma associated oncogene family zinc finger; HPE9; Oncogene GLI2; PHS2; Tax helper protein 1; Tax helper protein 2; Tax helper protein; Tax responsive element 2 holding protein; Tax responsive element 25 bp sequence binding protein; THP; THP1; THP2; Zinc finger protein GLI2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    42.5kDa
  • 表达区域：
    412-641aa
  • 氨基酸序列
    EQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Required for normal embryonic development.; Involved in the smoothened (SHH) signaling pathway.; Involved in the smoothened (SHH) signaling pathway.; Involved in the smoothened (SHH) signaling pathway.; Involved in the smoothened (SHH) signaling pathway.; Acts as a transcriptional activator in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1.; (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1.; (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1.; (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1.; Acts as a transcriptional repressor.
  • 基因功能参考文献：
    1. Importantly, high expression levels of HIF-1alpha/TGF-beta2/GLI2 correlated robustly with the patient relapse following chemotherapy, highlighting a potential biomarker and therapeutic target for chemoresistance in colorectal cancer. PMID: 29891662
    2. Study found increased expression of GLI2 in osteosarcoma tissues and cell lines and determined that GLI2 is a target of miR-141-3p. PMID: 29251328
    3. In conclusion, our data suggest that overexpression of the Hedgehog components SHH, GLI2 and FOXA2 might be used as markers of an aggressive hemangioma. PMID: 28370639
    4. High GLI2 or PDGFRB expression is associated with unfavorable survival in GC patients. GLI2 can induce PDGFRB expression in GC cells via directly binding to its promoter. In addition, the GLI2-PDGFRB axis might be an important signaling pathway modulating CSC properties of GC cells. PMID: 28975979
    5. Study report that compromised CSL function depends on GLI activation for conversion of human dermal fibroblasts into CAFs, separately from cellular senescence. Decreased CSL upregulates the expression of the ULK3 kinase, which binds and activates GLI2. PMID: 28877478
    6. The s found that hepatitis C virus derived from hepatitis C patients infected and directly induced the trans-differentiation of human primary fibroblasts into myofibroblasts, promoting fibrogenesis. This effect correlated with the activation of GLI2, one of the targets of Hedgehog signaling pathway previously reported to be involved in myofibroblast generation. PMID: 27476557
    7. role of GLI2-ABCG2 signaling axis for 5Fu resistance in gastric cancer PMID: 28847472
    8. This study showed associations of anorectal malformations with rs3738880 in GLI2 and with previous miscarriages. PMID: 28057877
    9. High GLI2 expression is associated with hepatocellular carcinoma. PMID: 27036048
    10. our findings demonstrate that multiple signaling pathways converge on Gli2 to mediate PTHrP expression and bony invasion, highlighting Gli2 as a therapeutic target to prevent bony invasion in OSCC. PMID: 27738315
    11. the effect gene of the Shh pathway, gli1, was found to have a reduced level of expression along with a decreased expression of gli2. PMID: 26446020
    12. High Gli2 expression is associated with non-small cell lung cancer. PMID: 26936993
    13. GLI2 is a dosage-sensitive gene that may be responsible for the agenesis of corpus callosum observed in the proband. PMID: 27346851
    14. Data suggest that Nrp1 (neuropilin-1) regulates Hedgehog signaling specifically at the level of activation of GLI2 transcriptional activator function; Nrp1 localization to primary cilium does not correlate with Hedgehog signal promotion. These studies were conducted in various cell types. PMID: 28667171
    15. studies identify a novel molecular mechanism of regulation of CD40L by the transcription factor GLI2 in the tumor microenvironment downstream of CCR3 signaling PMID: 28461568
    16. the data suggest that CCRK positively regulates the kinetics by which ciliary proteins such as Smoothened and Gli2 are imported into the cilium, and that the efficiency of ciliary recruitment allows for potent responses to Hedgehog signaling over long time periods. PMID: 28817564
    17. GLI2 is a regulator of beta-catenin and provides insights into its role in tumorigenesis. PMID: 28300597
    18. bioinformatics analysis and luciferase reporter assay predicted the direct binding to miR-125b-5p, which has been reported to function as a tumor suppressor in osteosarcoma. Furthermore, functional experiments validated that cir-GLI2 exerted the tumor-promoting effects on osteosarcoma cells via negatively targeting miR-125b-5p. PMID: 28695772
    19. These results demonstrate that miR-124 is an important downstream target gene of Hh signaling, and the Gli2/miR-124/AURKA axis is essential for the proliferation and growth of human glioma cells. PMID: 28393219
    20. in trophoblasts, GLI2 not only directly bound to the promoter region of gene hsd11b2 to transactivate hsd11b2 but also formed a heterodimer with RNA polymerase II, resulting in up-regulation of hsd11b2 gene transcription PMID: 27379371
    21. GLI2 was identified as a crucial downstream mediator of the pro-fibrotic effects of TGF-beta. PMID: 27793816
    22. this study shows that Gli2 expression was more often positive in advanced non-small-cell lung carcinoma patients refractory to platinum-based chemotherapy PMID: 26762562
    23. Beclin-1 acts as a tumor suppressor by regulating the hedgehog (HH) signaling pathway through Gli2 expression in gastric adenocarcinoma. PMID: 25512258
    24. Overexpression of GLI2 is associated with osteosarcoma progression. PMID: 27460090
    25. MALAT1 promotes gastric cancer proliferation and progression. MALAT1 is a direct target of miR-202 and knockdown of MALAT1 significantly decreases the expression of Gli2 by negatively regulating miR-202. PMID: 27887846
    26. the GLI2 gene may be one of the candidate genes to analyze when an association of pituitary hormone deficiency and developmental defects in posterior pituitary gland. The highly variable phenotype found suggests the presence of additional unknown factors that could contribute to the phenotype observed in these patients. PMID: 27576279
    27. the GLI2 protein level could serve as a feasible marker of ligand-dependent hedgehog activation in pancreatic neoplasms. PMID: 27543868
    28. ALDH1A1 stabilizes the Gli2 protein in an enzyme-independent manner in hepatocellular cancer. PMID: 26896768
    29. GLI2 stabilized glial cell missing-a, a pivotal transcriptional factor for trophoblastic syncytialization. PMID: 26769961
    30. The results do not support our hypothesis that common germline genetic variants in the GLI2 genes is associated with the risk of developing medulloblastoma. PMID: 26290144
    31. GLI2 mutations typically result in pituitary anomalies with or without postaxial polydactyly. PMID: 25974718
    32. Serum starvation significantly up-regulated the expression of Gli1, which in turn increased its key target cIAP2 expression and enhanced NF-kappaB/cIAP2 pathway, resulting in promoting cell survival under serum starvation PMID: 26182949
    33. the JQ1-resistant cells remain dependent on c-MYC that now becomes co-regulated by high levels of GLI2 PMID: 25807524
    34. A relatively high frequency of GLI2 mutations occur in patients with congenital growth hormone deficiency. [Review] PMID: 25878059
    35. hsa-miR-665 and hsa-miR-95 were downregulated in GSRCC but upregulated in intestinal gastric adenocarcinoma, and the relatively differential expression of the miRNAs negatively controlling their target genes, GLI2 and PLCG1 PMID: 25964059
    36. during vertebrate history GLI2 acquired a high level of complexity in the genetic mechanisms regulating its expression during spatiotemporal patterning of the central nervous system (CNS) and limbs. PMID: 25715918
    37. This family confirms the incomplete penetrance of genomic disturbances affecting the GLI2 gene. However, the family presented here is unique as none of the three identified individuals with a GLI2 deletion showed any typical signs of holoprosencephaly PMID: 25820550
    38. We genotyped the SLC6A4 5-HTTLPR (rs4795541, rs25531) and TPH2 1125A>T (rs4290270) variants and evaluated their role in moderating disulfiram treatment for cocaine dependence. PMID: 22925276
    39. miR-218 and its downstream target Gli2, as well as E-cadherin, participate in the anti-invasion process PMID: 25694126
    40. GLI2 forms part of a core HH pathway transcriptional regulatory network that promotes human myeloid leukemic progression and dormant LSC generation PMID: 25889765
    41. SPOP plays critical roles in suppressing gastric tumorigenesis through inhibiting Hh/Gli2 signaling pathway. It may provide an alternative strategy for developing therapeutic agents of gastric cancer in future. PMID: 25204354
    42. Gli2 silencing enhances TRAIL-induced apoptosis and reduces tumor growth in human hepatoma cells in vivo. PMID: 25535898
    43. results indicate that miR-202 acts as a novel tumor suppressor to regulate OS cell proliferation and apoptosis through downregulating Gli2 expression. PMID: 25156120
    44. GLI2 regulated metastasis as well as the progression of osteosarcomas. PMID: 25082385
    45. The 14-3-3zeta-driven contextual changes of Smad partners from p53 to Gli2 may serve as biomarkers and therapeutic targets of TGF-beta-mediated cancer progression PMID: 25670079
    46. Report shows that GLI2 is the novel target gene of DeltaNp63alpha and that DeltaNp63alpha-GLI2 crosstalk in osteosarcoma cells is a necessary event in osteosarcoma progression. PMID: 25085524
    47. Increased GLI2 expression is associated with osteosarcoma invasion. PMID: 25449781
    48. Individuals with truncating mutations in GLI2 typically present with pituitary anomalies, polydactyly and subtle facial features rather than holoprosencephaly PMID: 24744436
    49. High Gli2 expression is associated with bone destruction in lung and breast cancer. PMID: 25359619
    50. Our studies demonstrate an important role for GLI2 in Lung squamous cell carcinoma PMID: 24423612

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  • 相关疾病：
    Holoprosencephaly 9 (HPE9); Culler-Jones syndrome (CJS)
  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell projection, cilium.; [Isoform 1]: Nucleus.; [Isoform 2]: Nucleus.
  • 蛋白家族：
    GLI C2H2-type zinc-finger protein family
  • 组织特异性：
    Expressed in breast cancers (at protein level). Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly ex
  • 数据库链接：

    HGNC: 4318

    OMIM: 165230

    KEGG: hsa:2736

    STRING: 9606.ENSP00000354586

    UniGene: Hs.111867