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  4. Recombinant Human Zinc finger protein 148 (ZNF148)

Recombinant Human Zinc finger protein 148 (ZNF148)

  • 货号：
    CSB-YP026544HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026544HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026544HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026544HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026544HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ZNF148
  • Uniprot No.：
  • 别名：
    Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-794
  • 氨基酸序列
    MNIDDKLEGL FLKCGGIDEM QSSRTMVVMG GVSGQSTVSG ELQDSVLQDR SMPHQEILAA DEVLQESEMR QQDMISHDEL MVHEETVKND EEQMETHERL PQGLQYALNV PISVKQEITF TDVSEQLMRD KKQIREPVDL QKKKKRKQRS PAKILTINED GSLGLKTPKS HVCEHCNAAF RTNYHLQRHV FIHTGEKPFQ CSQCDMRFIQ KYLLQRHEKI HTGEKPFRCD ECGMRFIQKY HMERHKRTHS GEKPYQCEYC LQYFSRTDRV LKHKRMCHEN HDKKLNRCAI KGGLLTSEED SGFSTSPKDN SLPKKKRQKT EKKSSGMDKE SALDKSDLKK DKNDYLPLYS SSTKVKDEYM VAEYAVEMPH SSVGGSHLED ASGEIHPPKL VLKKINSKRS LKQPLEQNQT ISPLSTYEES KVSKYAFELV DKQALLDSEG NADIDQVDNL QEGPSKPVHS STNYDDAMQF LKKKRYLQAA SNNSREYALN VGTIASQPSV TQAAVASVID ESTTASILES QALNVEIKSN HDKNVIPDEV LQTLLDHYSH KANGQHEISF SVADTEVTSS ISINSSEVPE VTPSENVGSS SQASSSDKAN MLQEYSKFLQ QALDRTSQND AYLNSPSLNF VTDNQTLPNQ PAFSSIDKQV YATMPINSFR SGMNSPLRTT PDKSHFGLIV GDSQHSFPFS GDETNHASAT STQDFLDQVT SQKKAEAQPV HQAYQMSSFE QPFRAPYHGS RAGIATQFST ANGQVNLRGP GTSAEFSEFP LVNVNDNRAG MTSSPDATTG QTFG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
  • 基因功能参考文献：
    1. Our findings suggested that ZBP-89 and Sp1 overexpression induced Bak expression in a genetic manner. Increased Bak level was associated with poor patient survival, whereas high level of Sp1 is a beneficial factor for patient survival. PMID: 29653560
    2. The results indicate that the association with chr5p15.33-Region 2 may be explained by rs36115365, a variant influencing TERT expression via ZNF148 in a manner consistent with elevated TERT in carriers of the C allele. PMID: 28447668
    3. ZNF148 as a gene involved in a newly described intellectual disability syndrome with a recurrent phenotype and postulate that the ZNF148 is a hitherto unrecognized but crucial transcription factor in the development of the corpus callosum. PMID: 27964749
    4. A larger role ZBP-89 plays in gene regulation during inflammation.ZBP-89 regulates of MMP-1 expression.ZBP-89 and NF-kappaB appear to bind cooperatively on both promoters. PMID: 26891870
    5. ZBP-89 attenuates HDAC3 by increasing IkappaB degradation, dependent on Pin1 but independent of NF-Kappab PMID: 25623232
    6. zinc-binding protein-89 upregulates the expression of Bak by targeting multiple components of the epigenetic pathway in hepatocellular carcinoma. PMID: 23954442
    7. lower expression of ZNF148 in colorectal cancer was significantly associated with worse clinicopathologic variables, including lymph node metastases, poor differentiation, and a higher rate of disease recurrence PMID: 23576061
    8. this study provides vigorous evidence that ZBP-89 was significantly downregulated in clear cell renal cell carcinoma(CCRCC) and could be served as a promising biomarker for prediction of distal metastasis and prognosis of patient with CCRCC. PMID: 22982674
    9. Colony formation was reduced dramatically in those hepatocellular carcinoma cell lines in which ZBP-89 overexpression was demonstrated; this appeared to correlate with increased apoptosis. PMID: 22372401
    10. We found that the TP53-G245D variant but not TP53-R249S abrogated HDAC inhibitor p21 induction by binding to ZBP-89 and retaining it in the cytoplasm. PMID: 22214764
    11. ZBP-89 is a regulator of Pdcd4 gene, binding to the basal promoter either alone or by interacting with Sp family members. PMID: 22111549
    12. An activating role for ZBP-89 in human globin gene regulation and erythroid differentiation. PMID: 21828133
    13. Alternative binding of ZBP89 or SP1 to the described region in the IRS2 promoter regulates neuronal IRS2 expression in a PI3K-dependent manner. PMID: 19875459
    14. ZBP-89 is a repressor of the human beta 2-integrin CD11b gene during differentiation of monocytes into macrophages PMID: 12393719
    15. ZBP-89 co-localized with p53 in the nucleus in about 67% (12 of 18) of all cases positive for the nuclear p53 protein, suggesting that ZBP-89 may play a role in the nuclear accumulation of the p53 protein in a subset recurrent hepatocellular carcinoma. PMID: 12759240
    16. interaction of Stat3 and ZBP-89 may be crucial for overcoming the effects of the repressor ZBP-89, which suggests a novel mode for Stat3 gene activation. PMID: 14712222
    17. ZBP-89-mediated apoptosis occurs via a p53-independent mechanism that requires JNK activation. PMID: 14963412
    18. No association with psoriasis susceptibility PMID: 15175029
    19. We conclude that ZBP-89 is a direct transcriptional activator of the enterocyte differentiation marker IAP. PMID: 16384873
    20. Ectopic expression of ZBP-89 amplified the inhibitory effect of Fatty Acids on L2 -GH promoter activity. PMID: 16825291
    21. ZBP-89 is an important co-activator of wild-type p53 and both proteins are negatively affected by functionally inactive p53 mutants. PMID: 16827139
    22. Enhanced ZNF148 expression activates intestinal apoptosis, therefore ZNF148 is a therapeutic target to inhibit colon cancer development. PMID: 17019648
    23. ataxia telangiectasia mutated protein phosphorylation of ZBP-89 contributes to Histone deacetylase inhibitors induction of p21(waf1) gene expression PMID: 17560543
    24. sumoylation provides a reversible post-translational mechanism to control the activity of ZBP-89. PMID: 17940278
    25. Transcription factors Sp3, ZBP-89 and NF-Y are capable of binding to the SOX18 promoter region. PMID: 18496767
    26. both NF-kappaB and ZBP-89 bind to the site in vivo and provide evidence of competitive binding to MMP-3 promoter. PMID: 19275880
    27. ZBP-89 greatly enhanced the killing effectiveness of 5-fluorouracil or staurosporine in hepatocellular carcinoma cells. PMID: 19362768
    28. ZBP-89 iss able to restrain senescence in NCI-H460 human lung cancer cells, through epigenetically regulating p(16INK4a) expression. PMID: 19583777
    29. ZBP-89 plays a role in erythroid and megakaryocytic development by cooperating with GATA-1 and/or FOG-1 in a developmental stage-specific manner. PMID: 18250154

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  • 相关疾病：
    Global developmental delay, absent or hypoplastic corpus callosum, and dysmorphic facies (GDACCF)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 数据库链接：

    HGNC: 12933

    OMIM: 601897

    KEGG: hsa:7707

    STRING: 9606.ENSP00000353863

    UniGene: Hs.592591