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  4. Recombinant Human Xaa-Pro dipeptidase (PEPD)

Recombinant Human Xaa-Pro dipeptidase (PEPD)

  • 货号：
    CSB-YP017784HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017784HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017784HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017784HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017784HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PEPD
  • Uniprot No.：
  • 别名：
    Aminoacyl L proline hydrolase; Imidodipeptidase; MGC10905; MGC95081; Pep 4; Pep4; pepD; PEPD_HUMAN; Peptidase 4; Peptidase D; Prolidase; Proline dipeptidase; X pro dipeptidase; X-Pro dipeptidase; Xaa-Pro dipeptidase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-493
  • 氨基酸序列
    AAATGPSFW LGNETLKVPL ALFALNRQRL CERLRKNPAV QAGSIVVLQG GEETQRYCTD TGVLFRQESF FHWAFGVTEP GCYGVIDVDT GKSTLFVPRL PASHATWMGK IHSKEHFKEK YAVDDVQYVD EIASVLTSQK PSVLLTLRGV NTDSGSVCRE ASFDGISKFE VNNTILHPEI VECRVFKTDM ELEVLRYTNK ISSEAHREVM KAVKVGMKEY ELESLFEHYC YSRGGMRHSS YTCICGSGEN SAVLHYGHAG APNDRTIQNG DMCLFDMGGE YYCFASDITC SFPANGKFTA DQKAVYEAVL RSSRAVMGAM KPGVWWPDMH RLADRIHLEE LAHMGILSGS VDAMVQAHLG AVFMPHGLGH FLGIDVHDVG GYPEGVERID EPGLRSLRTA RHLQPGMVLT VEPGIYFIDH LLDEALADPA RASFLNREVL QRFRGFGGVR IEEDVVVTDS GIELLTCVPR TVEEIEACMA GCDKAFTPFS GPK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
  • 基因功能参考文献：
    1. PEPD directly binds to p53 in the nucleus and cytoplasm and suppresses both transcription-dependent and transcription-independent activities of p53, which does not require PEPD enzymatic activity. PMID: 29233996
    2. Increased serum prolidase activity and decreased antioxidant levels are likely to be a results of increased of oxidative stress levels in obese subjects PMID: 28867357
    3. Serum prolidase activity is reduced in scleroderma patients, especially in diffuse form. PMID: 28534076
    4. Increased levels of serum prolidase activity are associated with the severity of developmental dysplasia of the hip. PMID: 27320745
    5. High resolution X-ray crystallographic models of substrate and product binding in the active site of human prolidase. PMID: 28677335
    6. We report here that by introducing either the A252R or P365R substitution mutation, the structural changes affecting catalytic turnover rate and substrate binding affinity are valuable in improving the catalytic activity of human prolidase towards toxic organophosphorus compound hydrolysis. PMID: 28462712
    7. In ankylosing spondylitis, serum prolidase level was successful in measuring disease activity PMID: 27443556
    8. there is an independent relationship of aortic prolidase activity and aortic prolidase immunostaining with aortic stiffness beta index in patients who underwent coronary artery bypass grafting PMID: 27387023
    9. Prolidase activity decreases in patients with chronic obstructive pulmonary disease. PMID: 26870880
    10. absence of prolidase activity in pterygium tissue indicates that there is no collagen turnover in this tissue PMID: 26509313
    11. Decreased serum prolidase activity may be associated with knee osteoarthritis PMID: 25994092
    12. The association between the PEPD genetic variant and the risk of T2D was modulated by n-3 fatty acids. Higher n-3 fatty acids may abolish the adverse effect of the risk allele at PEPD for T2D PMID: 26087900
    13. Prolidase was required for IFNAR1 maturation and accumulation, activation of IFNbeta-stimulated gene induction, and IFN-I-dependent viral control. PMID: 26159719
    14. Levels of both serum prolidase and AFP could contribute to the early diagnosing of hepatocellular carcinoma. PMID: 26078578
    15. Serum prolidase level was significantly higher in schizophrenia patients compared to the controls. PMID: 25842545
    16. There may be an ethnic- and/or sex-specific association of the PEPD rs731839 single nucleotide polymorphism and serum lipid levels in Mulao and Han populations PMID: 25120796
    17. Prolidase is required for early trafficking events during influenza A virus entry. PMID: 25031340
    18. High prolidase activity may indicate critical biological activities relevant to pathological events in Behcet's disease, and this activity may be a biological indicator of disease. PMID: 24225260
    19. Significantly lower prolidase activity in patients with benign joint hypermobility syndrome suggests that prolidase may affect the collagen metabolism and cause hyperlaxity. PMID: 24612543
    20. Serum C4b, FN, and PEPD are associated with the pathological changes of pulmonary tuberculosis. PMID: 24484408
    21. Suggest prolidase as a high affinity ligand of the ErbB2 receptor which can regulate ErbB2 signaling and cell growth. PMID: 24810047
    22. Patients with psoriasis exhibit higher serum prolidase activity independent of gender, BMI, disease severity or duration, type of treatments or nitric oxide level. PMID: 23553128
    23. Data suggest that elevated serum prolidase activity and oxidative stress may be associated with increased cardiovascular risk in polycystic ovary syndrome (PCOS) and/or menstrual irregularities associated with this syndrome. PMID: 23163753
    24. Prolidase dependent HIF1A expression in breast cancer cells is induced by prolidase substrate peptides. PMID: 23549681
    25. prolidase may share an undefined role in fibrosis in heart failure and may have a role in the diffuse fibrosis of heart failure. PMID: 24065222
    26. Serum PEPD activity appears to be higher in patients with diabetic foot ulcers when compared with patients without diabetic foot ulcers and healthy controls. PMID: 23242638
    27. kinetic and structural evidences on human prolidase pathological mutants PMID: 23516557
    28. Varicose venous wall prolidase enzyme activity could be an important factor in progression of azoospermia and infertility in patients with varicocele. PMID: 23171426
    29. PEPD is a ligand of EGFR and presents a novel mechanism of EGFR activation PMID: 23212918
    30. A diminished prolidase activity may contribute to alteration of collagen metabolism and should be considered a biomarker of myeloproliferative neoplasms progress. PMID: 23457135
    31. Although approximately 70 PEPD gene mutations and polymorphisms have been reported in various ethnic groups, we however report, for the first time, the identification of insertion mutation in human the PEPD gene. PMID: 23287645
    32. A Mn(II)-Mn(II) center in human prolidase. PMID: 22999980
    33. Assessed the serum prolidase enzyme level and the oxidative-antioxidative status in chronic hepatitis C infection. The prolidase was higher in the chronic hepatitis C group compared to the control group. PMID: 22811354
    34. Increased prolidase seems to be associated with increased NO levels and oxidative stress along with decreased antioxidant levels in bladder cancer. PMID: 22258852
    35. Relinoic acid regulates prolidase activity in human dermal cells via IGF receptor signaling in photoaged cells. PMID: 22245250
    36. elevated in submucosal uterine fibroids PMID: 22185542
    37. Serum prolidase activity is elevated in patients with pulmonary tuberculosis PMID: 21722016
    38. Prolidase activity was positively correlated erum prolidase activity is significantly associated with Legg-Calve-Perthes disease. PMID: 21304409
    39. In the early pregnancy loss group compared to the control group, serum levels of prolidase activity were significantly lower and placental tissue activity levels were significantly higher. PMID: 20437180
    40. investigation of role of prolidase in regulation of expression of TGF beta1 & TGF beta1 receptor in skin fibroblasts: down-regulation by prolidase inhibitors (Cbz-Pro & PEP); up-regulation by prolidase products (Pro & HyPro) PMID: 20868675
    41. Serum prolidase activity seems to be correlated with the level of fibrosis in patients with non-alcoholic steatohepatitis. PMID: 20486204
    42. found serum prolidase activity was significantly lower in both Idiopathic and ischemic dilated cardiomyopathies (DCM) groups relative to healthy volunteers and lower in ischemic DCM than idiopathic. PMID: 20626024
    43. Data suggest that the presence of ascending aortic aneurysms is associated with low serum prolidase activity. PMID: 20383038
    44. Data shows prolidase activity was significantly increased in patients with thalassemia major compared with controls. PMID: 20087956
    45. FAK-independent regulation of prolidase activity and collagen biosynthesis in MCF-7 cells. PMID: 11820613
    46. Carbobenzoxyproline is a potent inhibitor of prolidase in cultured fibroblasts from patients with prolidase deficiency. PMID: 15878628
    47. Significant correlation was observed between serum prolidase activity, and total antioxidant capacity, total oxidant status and oxidative stress index (p<0.01, r=-0.367; p<0.05, r=0.283; p<0.01, r=0.379; respectively) in H. pylori-positive subjects. PMID: 16999949
    48. oxidative stress and decreased prolidase activity may have a role in pathogenesis of knee osteoarthritis PMID: 17096092
    49. Prolidase is viable target for selective activation of melphalan prodrugs in melanoma cells. PMID: 17377743
    50. Prolidase deficiency and systemic lupus erythematosus share a number of common immunological features. PMID: 17570078

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  • 相关疾病：
    Prolidase deficiency (PD)
  • 蛋白家族：
    Peptidase M24B family, Eukaryotic-type prolidase subfamily
  • 数据库链接：

    HGNC: 8840

    OMIM: 170100

    KEGG: hsa:5184

    STRING: 9606.ENSP00000244137

    UniGene: Hs.36473