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  4. Recombinant Human Wnt inhibitory factor 1 (WIF1)

Recombinant Human Wnt inhibitory factor 1 (WIF1)

  • 货号：
    CSB-YP026113HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026113HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026113HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026113HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026113HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    WIF-1; wif1; WIF1_HUMAN; Wnt inhibitory factor 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-379
  • 氨基酸序列
    GP PQEESLYLWI DAHQARVLIG FEEDILIVSE GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQAAGQAEYF YEFLSLRSLD KGIMADPTVN VPLLGTVPHK ASVVQVGFPC LGKQDGVAAF EVDVIVMNSE GNTILQTPQN AIFFKTCQQA ECPGGCRNGG FCNERRICEC PDGFHGPHCE KALCTPRCMN GGLCVTPGFC ICPPGFYGVN CDKANCSTTC FNGGTCFYPG KCICPPGLEG EQCEISKCPQ PCRNGGKCIG KSKCKCSKGY QGDLCSKPVC EPGCGAHGTC HEPNKCQCQE GWHGRHCNKR YEASLIHALR PAGAQLRQHT PSLKKAEERR DPPESNYIW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
  • 基因功能参考文献：
    1. WIF1 methylation may serve as a potential prognostic marker for patients with chondrosarcoma. PMID: 28484252
    2. our findings indicate that HOXC6 plays an important role in the progress and prognosis of human glioma and promotes glioma U87 cell growth through the WIF-1/Wnt signaling pathway PMID: 30228024
    3. Data revealed that the upregulation of WIF1 induced autophagy in non-small cell lung cancer (NSCLC) cells. WIF1-mediated autophagy was demonstrated to inhibit Wnt/betacatenin signaling by downregulating Dvl2, which contributed to the inhibition of the proliferation and the promotion of the apoptosis of NSCLC cells. PMID: 29916529
    4. WIF-1 gene methylation is associated with pathogenesis of multiple human tumors. PMID: 29970693
    5. the expression of WIF-1 was low in GBC cells due to aberrant hypermethylation of its promoter region. Additionally, an alternative pathogenesis of GBC was indicated in which c-Jun causes hypermethylation of the WIF-1 promoter region, and represses the expression of WIF-1 through transcriptional regulation and interaction with DNMT1 as an early event in the tumorigenesis of GBC. PMID: 29693707
    6. miR-590-3p regulates colon cancer progression via WIF1 and DKK1, which suggests that miR-590-3p may be a promising candidate for therapeutic applications in colon cancer treatment PMID: 29164578
    7. We identify a loss-of-function mutation in WIF1 (NM_007191 p.W15*), which is involved in mesoderm segmentation, as the suspected cause of the Nail-Patella-like disorder observed in this family. PMID: 28383544
    8. the results of our study show that down-regulated INTS6 expression is associated with a poorer prognosis in hepatocellular carcinoma (HCC) patients. This newly identified INTS6/WIF-1 axis indicates the molecular mechanism of HCC and may represent a therapeutic target in HCC patients. PMID: 28899352
    9. the promoters of WIF1, NLK, and APC are highly methylated in the nasopharyngeal cancers (NPC) and gastric carcinoma (GC) cell lines, and the 3 genes are also regulated by miR-BART19-3p expressed by Epstein-Barr virus (EBV); expression of the WIF1, APC, and NLK genes is strongly affected by hypermethylation, and in EBV-associated tumors, the 3 genes are also affected by miR-BART19-3p PMID: 28543390
    10. Data show the frequency of WIF-1 hypermethylation significantly increased in non-small cell lung cancer (NSCLC) specimens compared with normal lung tissue. WIF-1 hypermethylation is predominant in squamous cell carcinoma (SCC), suggesting that WIF-1 methylation contributes to the development of NSCLC, especially SCC. [review] PMID: 27911280
    11. Study supported the hypothesis that Wnt inhibitory factor 1 (WIF1) is crucial as a negative regulator of the functions of endothelial cells in angiogenesis and that hypoxia plays an important role in controlling WIF1 expression and angiogenesis. PMID: 26994065
    12. Our data suggests that total cellular b-catenin levels decrease in the presence of secreted frizzled-related protein 1 and Wnt inhibitory factor 1, and a significant increase in cell death after tyrosine kinase inhibitor treatment is observed. On the contrary, when secreted frizzled-related protein 1 is suppressed, total b-catenin levels increase in the cell and the cells become resistant to tyrosine kinase inhibitors. PMID: 28468589
    13. Gallbladder cancer patients with hypermethylated WIF-1 exhibited worse overall survival than those with hypomethylated WIF-1. PMID: 28438695
    14. In astrocytoma specimens, tumor areas with numerous single cells were identified which strongly express Wif-1. PMID: 28040794
    15. Hypermethylation of WIF1 (WNT inhibitory factor 1) and NPY (neuropeptide Y) genes was significantly higher in tumor tissue compared to normal tissue, independently of tumor stage. PMID: 27251038
    16. the expression levels of WIF-1 were low in gallbladder cancer tumor tissues and the GBC-SD, SGC-996 and NOZ gallbladder cancer cell lines. This low expression was associated with the methylation status of the WIF-1 gene promotor. PMID: 27430608
    17. Promoter hypermethylation WIF1 play an important role in the carcinogenesis of lung cancer. PMID: 27930522
    18. HOTAIR can affect the radiosensitivity of pancreatic ductal adenocarcinoma (PDAC) cells partly via regulating the expression of WIF-1, and HOTAIR-WIF-1 axis is a potential target for PDAC radiotherapy. PMID: 26482614
    19. beta-catenin expression may also be a poor prognostic factor for cervical cancer (CC) while WIF1 could be a potential drug target for treatment of advanced CC. PMID: 27843945
    20. Reduced expression of Wif-1 and increased expression of beta-catenin may be involved in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). PMID: 27655540
    21. Results provide evidence that WIF1 is frequently methylated in oral squamous cell carcinoma (OSCC), but not in oral submucous fibrosis tissues, which results in reduced expression. This epigenetic biomarker can be used for the early detection of OSCC. PMID: 26352791
    22. WIF-1 down-regulation may promote tumor cells invasion and metastasis in salivary gland adenoid cystic carcinoma. PMID: 26405993
    23. Loss of WIF1 enhances the migratory potential of glioblastoma through WNT5A that activates the WNT/Ca(2+) pathway and MALAT1. PMID: 25772239
    24. WIF1 has a role in breast neoplasms: its inhibition significantly relieves the cancer stem cell-limiting effects of dietary compound isoliquiritigenin PMID: 25918249
    25. Data show that the WIF domain of Wnt Inhibitory Factor 1 (WIF1) is bound by C-terminal domains of Wnt proteins Wnt5a and Wnt7a at two sites. PMID: 26342861
    26. detection of methylated Wif-1 in either S or U has a higher performance accuracy compared to guaiac FOBT for advanced colorectal neoplasia screening PMID: 25025467
    27. miR-603 regulates glioma development via Wnt-beta-cateninn signaling pathway and its WIF1 and CTNNBIP1 targets. PMID: 25681036
    28. Further evaluation of the methylation of DACH1, DKK1, and WIF1 in a clinical patient group confirmed the frequent methylation of WIF1 and intermediate or low frequency of methylation of DACH1 or DKK1, respectively. PMID: 25487617
    29. Altered expression of genes associated with telomere maintenance and cell function of human vascular endothelial cell at elevated temperature. PMID: 25142166
    30. Aberrant methylation of APC gene was statistically significant associated with age over 50, DDK3 with male, SFRP4, WIF1, and WNT5a with increasing tumor stage SFRP4 and WIF1 with tumor differentiation and SFRP2 and SFRP5 with histological type PMID: 25107489
    31. Coexpression of SFRP1 and WIF1, rather than SFRP1 or WIF1 alone, could be used, together with low TNM stage, as a prognostic predictor of favorable outcomes in CRC. PMID: 24949429
    32. MicroRNA-181a promotes tumor growth and liver metastasis in colorectal cancer by targeting the tumor suppressor WIF-1. PMID: 24755295
    33. WIF-1 is frequently methylated and that Wnt5a acts as a tumor suppressor gene in colorectal carcinoma. PMID: 24833087
    34. genetic alterations of beta-catenin and epigenetics-related Wif-1 promoter hypermethylation may be important mechanisms underlying adrenocortical tumor formation PMID: 24755523
    35. WIF1 downregulation is a frequent event in salivary gland carcinoma ex-pleomorphic adenoma. PMID: 24853424
    36. melanocytes express WIF-1 constitutively in vivo and in vitro and that WIF-1 promotes melanogenesis in normal human melanocytes. PMID: 24131586
    37. WIF-1 is downregulated and functions as a tumor suppressor by antagonizing Wnt/beta-catenin signaling in neuroblastoma. PMID: 24561119
    38. Wif1 methylation showed an association with unfavorable prognosis of adenocarcinoma (AC) patients with EGFR mutation. PMID: 23686431
    39. Promoter methylation of WIF1, PENK and NPY is associated with colorectal adenocarcinoma (CRC) diagnosis and can be used to CRC from other cancers. PMID: 24289328
    40. HOTAIR directly decreased WIF-1 expression by promoting its histone H3K27 methylation in the promoter region and then activated the Wnt/beta-catenin signaling pathway. PMID: 24118380
    41. Significantly higher methylation was observed in genes NTKR1, GATA4 and WIF1 in the ovarian cancer group compared with the control group. PMID: 23746174
    42. WIF1 expression is downregulated by promoter methylation and is an important mechanism of aberrant WNT/beta-catenin pathway activation in astrocytoma pathogenesis PMID: 23328978
    43. The miR-29 family (miR-29a, -29b, and -29c) suppresses the Wnt signaling pathway through demethylation of WIF-1 in non-small-cell lung cancer. PMID: 23939044
    44. These in vivo data support the involvement of Wnt signaling in the development of aortic stenosis and suggest that circulating Wnt modulators such as WIF1 should be further investigated as risk markers in larger aortic stenosis populations. PMID: 23316316
    45. WIF1 is a marker of interfollicular keratinocyte stem cells and inhibits their cell cycle progression even with Wnt3 signaling. PMID: 23358094
    46. Loss of WIF-1 expression due to methylation is related to aggressiveness of sporadic breast cancer. PMID: 23417837
    47. It was shown that WIF-1 downregulation is involved in melasma development due to the stimulation of melanogenesis and melanosome transfer through upregulation of the canonical and the noncanonical Wnt signaling pathway. PMID: 22951732
    48. This study demonistrated that Wnt inhibitory factor-1 regulates glioblastoma cell cycle and proliferation. PMID: 22901505
    49. Epigenetic inactivation of Wnt inhibitory factor-1 is associated with esophageal squamous cell carcinoma. PMID: 23193918
    50. A Wnt-binding site of the WIF-domain of WIF1 was localized. Substitution of some residues of WIF resulted in an increased affinity for Wnt5a, but decreased affinity for Wnt3a, suggesting that these residues may define the specificity of WIF for Wnts. PMID: 22986341

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 18081

    OMIM: 605186

    KEGG: hsa:11197

    STRING: 9606.ENSP00000286574

    UniGene: Hs.284122