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  4. Recombinant Human WNT1-inducible-signaling pathway protein 1 (WISP1)

Recombinant Human WNT1-inducible-signaling pathway protein 1 (WISP1)

  • 货号：
    CSB-YP026119HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026119HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026119HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026119HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026119HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CCN4; WISP1; CCN family member 4; WNT1-inducible-signaling pathway protein 1; WISP-1; Wnt-1-induced secreted protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    23-367
  • 氨基酸序列
    TALSPAPT TMDFTPAPLE DTSSRPQFCK WPCECPPSPP RCPLGVSLIT DGCECCKMCA QQLGDNCTEA AICDPHRGLY CDYSGDRPRY AIGVCAQVVG VGCVLDGVRY NNGQSFQPNC KYNCTCIDGA VGCTPLCLRV RPPRLWCPHP RRVSIPGHCC EQWVCEDDAK RPRKTAPRDT GAFDAVGEVE AWHRNCIAYT SPWSPCSTSC GLGVSTRISN VNAQCWPEQE SRLCNLRPCD VDIHTLIKAG KKCLAVYQPE ASMNFTLAGC ISTRSYQPKY CGVCMDNRCC IPYKSKTIDV SFQCPDGLGF SRQVLWINAC FCNLSCRNPN DIFADLESYP DFSEIAN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
  • 基因功能参考文献：
    1. Urothelial cell carcinoma carrying WISP1 rs2977530 genetic variants (AG + GG) have a higher risk of developing a more invasive tumor stage and a large tumor. PMID: 29277583
    2. Study shows that WISP1 expression is significantly upregulated in glioblastoma tissue and cell lines. Also, WISP1 seems to be an important regulator of glioblastoma cell proliferation, apoptosis, migration, invasion, and TMZ drug resistance. WISP1 mediates these biological processes through regulating critical gene expression and pathways that are involved in cancer progression. PMID: 28902353
    3. WISP1 has a role in colon cancer apoptosis, invasion and poor prognosis PMID: 27409174
    4. Results indicate that SFRP1 rs7832767 C > T, CTNNB1 rs2293303 C > T, and WISP1 rs16893344 C > T were all strongly correlated with myocardial infarction (MI) susceptibility. PMID: 27391264
    5. There is a relationship between WISP1 and the metabolic parameters of gestational diabetes (GDM). And, WISP1 might be involved in the pathophysiology of GDM. PMID: 27267804
    6. Macrophage-derived IL-10 has a role in mediating mucosal repair by epithelial WISP-1 signaling PMID: 28783045
    7. results demonstrate that a joint effect of WISP1 rs2929970 with smoking as well as WISP1 rs16893344 with betel nut chewing causally contributes to the occurrence of OSCC. WISP1 polymorphism may serve as a marker or a therapeutic target in OSCC PMID: 28426731
    8. Taken together, our findings presented the first evidence that Wnt1-inducible signaling pathway protein-1 was upregulated in gastric cancer and acted as an oncogene by promoting proliferation, migration, and invasion in gastric cancer cells. PMID: 28618940
    9. Akt signaling pathway mediates WISP1-induced migration and proliferation of human VSMCs PMID: 27321870
    10. WISP1 is increased in IBD and contributes to the proinflammatory cascades in the gut. PMID: 27403031
    11. WISP1-induced IL-6 expression contributes to the pro-proliferative effect on fibroblasts. PMID: 26867691
    12. WISP1 plays an important role in regulating proliferation and apoptosis of Jurkat cells. PMID: 26823919
    13. Study showed that DNA methylation of the WISP1 promoter in the majority of the oral squamous cell carcinoma explains its expression regulation. PMID: 26391330
    14. CCN4 has a positive influence on chondrogenic differentiation by modulating the effects of TGF-beta3. PMID: 26555637
    15. Its role in tumor progression and clinical outcome makes WISP1 an emerging candidate for the detection and treatment of tumors. [review] PMID: 25794425
    16. WISP1 contributed to radioresistance primarily by repressing irradiation-induced DNA damage and activating PI3K kinase. PMID: 25749038
    17. WISP1 functions as an oncogene for human breast cancer. Ectopic expression of WISP1 in breast cancer cells promotes cell growth and metastasis. PMID: 25732125
    18. WISP1 polymorphisms (rs2929965, rs2929969, rs2929970, rs2929973 and rs754958) were related to the overall platinum-based chemotherapy toxicity of lung cancer in subgroup analyses. PMID: 25405734
    19. Our data indicate that WISP-1 plays an important role in the development of radioresistance in esophageal cancer cells during fractionated irradiation. PMID: 24728101
    20. CCN4 activates signalling pathways in osteoarthritis synovial fibroblasts leading to up-regulation of IL-6 production PMID: 23343403
    21. The data suggest that WISP1 may play a role in linking obesity to inflammation and insulin resistance and could be a novel therapeutic target for obesity. PMID: 25281430
    22. our findings indicate a regulatory role of miR-92a for WISP1 expression in pulmonary fibrosis. PMID: 24953558
    23. Wnt5a-induced Wnt1-inducible secreted protein-1 suppresses vascular smooth muscle cell apoptosis induced by oxidative stress. PMID: 25212236
    24. Data indicate that down-regulation of WNT-induced secreted protein 1 (WISP1/CCN4) in mesenchymal stromal cells resulted in cell death. PMID: 25200494
    25. WISP-1 enhances the migration of osteosarcoma cells by increasing MMP-2 and MMP-9 expression through the integrin receptor, Ras, Raf-1, MEK, ERK, and NF-kappaB signal transduction pathway. PMID: 24036215
    26. WISP-1 has a role in invasive breast cancer oncogenesis and reduced type 1 cell-mediated cytotoxic immunity PMID: 24426833
    27. WISP1/CCN4 expression in prostate cancer tissues was up-regulated in early stages of the disease. PMID: 23977121
    28. WISP1 is up-regulated by amyloid-beta-protein and can modulate its endogenous expression for the protection of microglia during apoptosis. PMID: 22873724
    29. Beta-catenin-dependent expression of WISP1 and Cyr61 is critical for epithelial repair. PMID: 23316072
    30. Prolonged exposure of kidney cells to ochratoxin A, expectable in human kidney due to a normal diet, leads to a marked ERK1/2-dependent upregulation of WISP1 gene expression, which, accompanied by increased ERK1/2- dependent TNF-alpha expression. PMID: 22778029
    31. CTGF and WISP-1 could play an important role in the progression of primary lung cancers by either individual gene itself or cointeraction. PMID: 19069651
    32. This study shows that constitutive activation of the Notch1 pathway confers fibroblasts with a suppressive phenotype to melanoma growth, partially through WISP-1. PMID: 21516124
    33. IL-18 induces signaling events that result in WISP1-mediated saphenous vein smooth muscle proliferation, survival and MMP induction that are key components of vein graft stenosis. This may be amplified by IL-18 and WISP1 autoregulation/cross-regulation. PMID: 21321938
    34. results indicated that WISP-1 enhances the migration of chondrosarcoma cells by increasing MMP-2 expression through the alpha5beta1 integrin receptor, FAK, MEK, ERK, p65 and NF-kappaB signal transduction pathway PMID: 21453685
    35. The expression of WISP-1 may play an important role in the progression of esophageal squamous cell carcinoma. PMID: 21498727
    36. WISP-1 expression in human breast cancer increases significantly and may play a key role in invasion and metastasis. PMID: 20506641
    37. WISP1 is mainly expressed during organ development and under diseased conditions, such as fibrosis or cancer. PMID: 21109017
    38. WISP-1 has a positive influence on bone cell differentiation and function and may work by enhancing the effects of BMP-2 PMID: 20684029
    39. WISP1 inhibits doxorubicin-induced p53 activation, p38 MAPK and JNK phosphorylation, Bax translocation to mitochondria, and cytochrome c release into cytoplasm. PMID: 20074638
    40. Upregulation of the WISP-1 is associated with lung cancer. PMID: 17579708
    41. The CCN4/WISP1 was upregulated in the plexiform neurofibromas. PMID: 20010302
    42. Elevated levels of connective tissue growth factor, WISP-1, and CYR61 in primary breast cancers associated with more advanced features. PMID: 11751417
    43. Overexpression of WISP1 is associated with breast cancer PMID: 11855747
    44. overexpression downregulates motility and invasion of lung cancer cells through inhibition of Rac activation PMID: 12529380
    45. WISP1v-mediated signaling is involved in the generation of invasive cellular properties and leads to progression of cholangiocarcinoma. PMID: 12717393
    46. Findings indicate that the WISP1v splicing variant plays a critical role in chondrocyte differentiation, whereas the HCS-2/8-specific splicing variant WISP1vx may be associated with the transformed phenotypes of chondrosarcomas. PMID: 17381509
    47. WISP-1 transcripts were found in lower levels in node-positive tumors compared with node negative tumors;were lower in patients with a moderate and poor Nottingham Prognostic Index prognosis compared with good prognostic groups. PMID: 17406949
    48. A genetic variation in the WISP1 gene locus is associated with spinal osteoarthritis. PMID: 17593496
    49. Aberrant levels of WISP-1 expression may play a role in rectal tumorigenesis. WISP-1 may be used as a specific clinical diagnosis and prognosis marker in rectal cancer. PMID: 17657846
    50. EpCAM is a Wnt-beta-catenin signaling target gene PMID: 18006828

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    CCN family
  • 组织特异性：
    Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer
  • 数据库链接：

    HGNC: 12769

    OMIM: 603398

    KEGG: hsa:8840

    STRING: 9606.ENSP00000250160

    UniGene: Hs.492974