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  4. Recombinant Human Vesicular acetylcholine transporter (SLC18A3), partial

Recombinant Human Vesicular acetylcholine transporter (SLC18A3), partial

  • 货号：
    CSB-YP613686HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP613686HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP613686HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP613686HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP613686HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    AChR; MGC12716; rVAT; Slc18a3; Solute carrier family 18 (vesicular acetylcholine) member 3; Solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; VAChT; VACHT_HUMAN; Vesicular acetylcholine transporter
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in acetylcholine transport into synaptic vesicles.
  • 基因功能参考文献：
    1. The results of this study suggest that the Gly360Arg mutant VAChT protein undergoes posttranslational degradation. PMID: 28188302
    2. PCMC expression of ADAM29, FLRT2, and SLC18A3 could be assessed as part of a routine screen to help identify individuals at risk of severe Obstructive sleep apnea in Asian populations PMID: 24732660
    3. Expression of VAChT is increased in neuronal cell lines following upregulation of Lhx8. PMID: 24316404
    4. alpha-Synuclein expression in axons to the distal gut correlates closely with expression of the cholinergic marker, VAChT. PMID: 22821666
    5. Data indicate that siRNA-mediated attenuation of vesicular acetylcholine transporter (VAChT, SLC18A3) reversed the apoptotic activity of vesamicol. PMID: 23222296
    6. Multiple abnormalities with intellectual and developmental disability result from recurrent deletions and reciprocal duplications of 10q11.21q11.23 including CHAT and SLC18A3. PMID: 21948486
    7. overexpressed ChAT enhanced transcription of the CHT1 gene but not the VACHT gene PMID: 21163949
    8. Mutations in the vesicular acetylcholine transporter demonstrate decreased affinity for acetylcholine and vesamicol. PMID: 20831599
    9. The colocalisation of CHT1 immunoreactivity with VAChT immunoreactivity in cholinergic enteric nerves in the human bowel thus suggests that CHT1 represents another marker of cholinergic nerves. PMID: 20490865
    10. Three non-coding SNPs were detected in SLC18A3. None demonstrated any reproducible association with late-onset AD in our samples. PMID: 12759818
    11. An 11.2 kb transgene (named hV11.2) that spanned about 5 kb upstream of the start of VACHT translation down to the first choline acetyltransferase coding exon gave variable expression in the medial habenular nucleus of transgenic mice PMID: 14622097
    12. presence of VAChT in cutaneous nerves and in both epidermal melanocytes and keratinocytes as well as in their nuclei PMID: 16763548
    13. Time-dependent dissociation of bound [3H]vesamicol is biphasic, but equilibrium saturation curves are not. The contrasting phasicity suggests that the pathway to and from the [3H]vesamicol binding site exists in open and at least partially closed states. PMID: 19685929

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  • 相关疾病：
    Myasthenic syndrome, congenital, 21, presynaptic (CMS21)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Vesicular transporter family
  • 组织特异性：
    Peripheral and central cholinergic nervous systems.
  • 数据库链接：

    HGNC: 10936

    OMIM: 600336

    KEGG: hsa:6572

    STRING: 9606.ENSP00000363229

    UniGene: Hs.654374