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  4. Recombinant Human Upstream stimulatory factor 1 (USF1)

Recombinant Human Upstream stimulatory factor 1 (USF1)

  • 货号：
    CSB-YP025681HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025681HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025681HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025681HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    USF1
  • Uniprot No.：
  • 别名：
    USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; bHLHb11; Major late transcription factor 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-310
  • 氨基酸序列
    MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG LEVVIKNDSN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
  • 基因功能参考文献：
    1. Study did not show any associations between rs2516839 and rs3737787 polymorphisms of the USF-1 gene and pediatric ischemic stroke. However, found an influence of specific genotypes on the outcome of stroke, including epilepsy, dysarthria, and a decrease in intellectual functioning. PMID: 29598907
    2. our study first demonstrated that USF1 could activate the transcription of MUC13, thereby enhancing the proliferation and self-renewal of glioblastoma stem cells PMID: 29441861
    3. miR-483 is a self-regulating microRNA and can activate its own expression via USF1 PMID: 27693430
    4. Findings suggest the underlying mechanism of upstream transcription factor 1 (USF1) in papillary thyroid cancer (PTC) development. PMID: 26052935
    5. SNP (single-nucleotide polymorphism) alleles that reduced USF1 mRNA expression also displayed a beneficial cardiometabolic profile, featuring improved insulin sensitivity, a favorable lipid profile, and reduced atherosclerosis PMID: 26819196
    6. USF1 gene can take part in basal transcription regulation of the human A3G gene in hepatocyte, and the identified E-box represented a binding site for the USF1. PMID: 26772882
    7. A trend of increasing triglycerides levels in relation to the C allele dose of rs2516839 SNP was observed. The synergistic effect of cigarette smoking and C allele carrier state on CAD risk was found. PMID: 26068452
    8. Rta autoregulates BRLF1 transcription via its interaction with USF1 on E1. PMID: 26297580
    9. Results show that the USF1 and APOA5 polymorphisms are associated with Familial combined hyperlipidemia and that the S19W SNP in the APOA5 gene is associated to the disease independently of total cholesterol, triglycerides and body mass index. PMID: 25308402
    10. The carriers of minor alleles of rs2516839 (C) and rs1556259 (G) of USF1 gene had decreased USF1 expression in atherosclerotic plaques as compared to non-carriers. PMID: 24722012
    11. Our results provided a new insight into susceptibility of USF1 variant with male infertility. rs11204546 of OR2W3 and rs11677854 of GTF2A1L might be additional valuable molecular predictive markers PMID: 25374392
    12. CK2 phosphorylation of USF1 modulates two functionally important properties of USF1, namely hetero-dimerization and transactivation. PMID: 25194820
    13. Further studies may be needed to validate our results and gain insights into the pathological mechanism of USF1 gene in the HCC tumorigenesis. PMID: 25480412
    14. A significantly elevated genotype frequency in certain USF1 genes was observed in hepatocellular carcinoma patients. PMID: 25367853
    15. Increased upstream stimulatory factor in HCC is associated with heparanase expression. USF might be an important factor in regulating HPSE expression and act as a novel marker of metastatic recurrence of HCC patients PMID: 25149140
    16. They underscore the new role of USF1 and give new clues of how p53 loss of function can occur in any cell type. PMID: 24831529
    17. USF1 binding to an E-box element may be critical for basal MVP promoter activation. PMID: 24173679
    18. Knockdown of USF-1 protected the HepG2 cells from oxygen and glucose deprivation (OGD)-induced apoptosis. PMID: 24661879
    19. The rs2516838 and rs2516839 polymorphisms of USF1 influence total unstable CPA in atherosclerotic stroke patients, which might be new markers to predict the risk of recurrence for this disease. PMID: 23271305
    20. USF1 SNP's distribution and genetic risk for cardiovascular disease in Azoreans (Portugal) PMID: 24042129
    21. our findings reveal that the guided-recruitment of the hSET1A histone methyltransferase complex and its H3K4 methyltransferase activity by transcription regulator USF1 safeguards hematopoietic transcription programs PMID: 23754954
    22. These results suggest USF1 has an independent but gender- and age-associated effect on Alzheimer Disease-related brain lesion development. PMID: 22390463
    23. USF1 and USF2 are important regulatory factors for Alx3 gene expression in different cell types PMID: 23181698
    24. Transcription factors upstream stimulatory factor 1 (USF-1) and USF-2 are indispensable for the transcription of RORgammaT in human lymphocytes. PMID: 22891280
    25. Identify transcriptional circuitry whereby USF-1 variants influence hepatic triglyceride secretion in familial combined hyperlipidemia and related disorders. PMID: 22460558
    26. the loss of USF-1 compromises DNA repair, which suggests that USF-1 plays an important role in maintaining genomic stability. PMID: 22291606
    27. the T-1514C polymorphism affects TBX21 gene expression and Th1 cytokine production by binding USF-1 to the SNP site PMID: 22258560
    28. The E-box binding factors Max/Mnt, MITF, and USF1 act coordinately with FoxO to regulate expression of proapoptotic and cell cycle control genes by phosphatidylinositol 3-kinase/Akt/glycogen synthase kinase 3 signaling. PMID: 21873430
    29. These data indicate that an E-box motif (RBE1) within the core promoter in the long terminal repeat of HIV-1 is a bona fide binding site for the RBF-2 transcription factor complex USF1, USF2, and TFII-I. PMID: 21813151
    30. This study demonstrated the involvement of USF1 and USF2 as molecular targets of Helicobacter pylori and the key role of DNA methylation in their regulation. PMID: 20180799
    31. USF1 is expressed in the nuclei of nicotine-stimulated odontoblasts, but in the cytoplasm of unstimulated odontoblasts. PMID: 18070436
    32. Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts. PMID: 18307922
    33. there is an indication of an interaction between APOA5 and USF1 on the risk for metabolic syndrome PMID: 20054229
    34. Over-expression of human USF1 in transgenic mice influenced metabolic traits. PMID: 19995791
    35. Defective gene induction of USF1 variant by insulin contributes to the risk of developing dyslipidemia. PMID: 20031629
    36. Reinforce the roles of APOA5 and USF1 variants on cardiovascular phenotypes and suggest that both genes contribute to lipid levels and aortic atherosclerosis individually and possibly through epistatic effects. PMID: 19910639
    37. requirement of carboxy ester lipase gene E-box for activation binds upstream regulatory factor 1 and 2 PMID: 11945176
    38. Cooperative E-box regulation of human GLI1 by TWIST and this protein PMID: 11948912
    39. demonstrated a novel mechanism for USF activation, which contributes to differential vasopressin expression in lung cancer. PMID: 12403649
    40. data indicated that upstream-stimulatory-factor (USF) might be involved in the basal transcriptional machinery of apolipoprotein E by binding to a non-canonical E-box motif within the proximal promoter PMID: 12444925
    41. transfection of expression vectors containing USF-1 or USF-2 cDNAs activated CYP1A2 reporter gene activity, while a dominant-negative USF-2 expression vector blocked such activity. PMID: 12663044
    42. we report the molecular cloning and functional characterization of a novel alternative splicing variant of human USF1 (hUSF1), termed USF1/BD. PMID: 12851711
    43. These findings suggest that increased protein levels and DNA binding of USF1 and USF2 mediate the inhibitory effects of hypoxia and of Mash-2 on CYP19 gene expression in human placenta PMID: 12917334
    44. USF1 transcription is regulated by cha, a basic helix-loop-helix transcription factor PMID: 12923186
    45. USF1/2 activates the hTERT promoter exclusively in hTERT-positive cells in a manner that is enhanced by the coactivator p300 and attenuated upon inhibiting p38-MAP kinase, a known modulator of USF activity. PMID: 12970752
    46. USF and Sp1 proteins interact and regulate human deoxycytidine kinase promoter activity PMID: 14514691
    47. transcription factors upstream stimulatory factor (USF) and c-Myc may exert opposing effects on PIGR promoter activity PMID: 14644095
    48. encodes a transcription factor known to regulate several genes of glucose and lipid metabolism PMID: 14991056
    49. role in regulating transcription of many genes involved in lipid and glucose homeostasis PMID: 15054483
    50. the influencing feature of both glucose and lipid homeostasis showing case-control heterogeneity PMID: 15175273

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  • 相关疾病：
    Hyperlipidemia combined 1 (HYPLIP1)
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 12593

    OMIM: 144250

    KEGG: hsa:7391

    STRING: 9606.ENSP00000356999

    UniGene: Hs.414880