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  4. Recombinant Human Ubiquitin-conjugating enzyme E2 N (UBE2N)

Recombinant Human Ubiquitin-conjugating enzyme E2 N (UBE2N)

  • 货号：
    CSB-YP025470HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025470HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025470HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025470HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025470HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    UBE2N
  • Uniprot No.：
  • 别名：
    Bendless like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; Epididymis secretory protein Li 71; HEL-S-71; Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds; MGC131857; MGC8489; UBC 13; Ubc13; UbCH ben; UbcH-ben; UbcH13; UBCHBEN; Ube 2N; Ube2n; UBE2N_HUMAN; Ubiquitin carrier protein N; Ubiquitin conjugating enzyme E2 N; Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13); Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast); Ubiquitin conjugating enzyme E2N; Ubiquitin protein ligase N; Ubiquitin-conjugating enzyme E2 N; Ubiquitin-protein ligase N; Yeast UBC13 homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-152
  • 氨基酸序列
    MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.
  • 基因功能参考文献：
    1. Uev1A-Ubc13 complex catalyzes lysine63-linked ubiquitination of RHBDF2 to promote TACE maturation. PMID: 29069608
    2. The malin-laforin complex interacts physically and functionally with the ubiquitin conjugating enzyme E2-N (UBE2N). PMID: 26546463
    3. together with UBE2L3 and UBE2D2, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
    4. MiR-205 inhibits DNA damage repair by targeting ZEB1 and Ubc13. PMID: 25476932
    5. Ubiquitin-conjugating enzyme complex Uev1A-Ubc13 promotes breast cancer metastasis through nuclear factor-small ka, CyrillicB mediated matrix metalloproteinase-1 gene regulation. PMID: 25022892
    6. Ubc13 was dispensable for transforming growth factor beta (TGFbeta)-induced SMAD activation but was required for activation of non-SMAD signaling via TGFbeta-activating kinase 1 (TAK1) and p38 PMID: 25189770
    7. UBE2N inhibition induces neuroblastoma apoptotic cell death via activation of p53 and JNK pathways. PMID: 24556694
    8. overexpression of hsa-miR-4516 downregulates STAT3, p-STAT3, CDK6, and UBE2N proteins that are consistently upregulated in psoriasis and induces apoptosis in HaCaT cells. PMID: 24610393
    9. crystal structure of the Shigella flexneri OspI-Ubc13 complex at 2.3 A resolution; study reveals the molecular basis of Ubc13 deamidation by OspI, as well as a convergence of E2 recognition by bacterial and host proteins PMID: 23633953
    10. The structure of the Shigella flexneri OspI and human Ubc13 complex revealed that the interacting surfaces between OspI and Ubc13 are a hydrophobic surface and a complementary charged surface. PMID: 23542009
    11. Data show RING finger (RNF) E3 ubiquitin ligase RNF8 dimerizes and binds to E2 ubiquitin-conjugating complex Ubc13/Mms2 with formation of Lys-63 ubiquitin chains, whereas the RNF168 RING domain is a monomer and does not catalyze Lys-6 ubiquitylation. PMID: 22589545
    12. the crystal structure of human OTUB1 in complex with human UBC13 and MMS2 PMID: 22679021
    13. Data show that growth hormone receptor (GHR) endocytosis requires both ubiquitin-conjugating enzyme Ubc13 and the ubiquitin ligase COOH terminus of Hsp70 interacting protein (CHIP). PMID: 22433856
    14. the interation between RAP80 and MDC1 is dependent upon the UBC13 mediated ubiquitination of MDC1 at K-1977 PMID: 21622030
    15. entire coding region and splice junctions of RNF8, UBC13 and MMS2 genes were screened for mutations in affected index cases from 123 Northern Finnish breast cancer families PMID: 21774837
    16. An NMR-based model of the ubiquitin-bound human complex and the structural basis of lysine 63 chain catalysis. PMID: 12569095
    17. Ubc13-Mms2 and Lys63-polyubiquitin chains are associated with signaling cellular stress PMID: 14562038
    18. The results in this study allowed identification of important residues of the Ubc13-Mms2 interface, determine a correlation between heterodimer formation and function, and conclude why Mms2 forms a specific complex with Ubc13 but not other Ubc proteins. PMID: 15749714
    19. ISGylation of Ubc13 disrupts its ability to form thioester bond with ubiquitin PMID: 16122702
    20. These data reveal a critical and unexpected role for vertebrate Ubc13 in the initiation of homologous recombination at the level of double-strand break processing. PMID: 17349954
    21. modification of proteins with Lys(63)-linked ubiquitin chains occurs through a UEV1A-independent substrate modification and UEV1A-dependent Lys(63)-linked ubiquitin chain synthesis mechanism PMID: 17709375
    22. Collectively, our results reveal that Ubc13 is essential for Tax ubiquitination, its interaction with NEMO, and Tax-mediated NF-kappaB activation. PMID: 17942533
    23. the human Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage PMID: 18077395
    24. RNF5 associates with JAMP in the ER membrane. This association results in Ubc13-dependent RNF5-mediated noncanonical ubiquitination of JAMP. PMID: 19269966
    25. crystal structures of TRAF6 and its complex with the ubiquitin-conjugating enzyme (E2) Ubc13 PMID: 19465916
    26. regulation of p53 multimerization that requires the concerted action of JNK and Ubc13 on polysome-bound p53. PMID: 19651615
    27. Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
    28. This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. PMID: 19034380
    29. This paper describes the function of the mouse protein, but suggests the function of the human protein, since both human and mouse proteins are 100% identical. PMID: 12039045

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 数据库链接：

    HGNC: 12492

    OMIM: 603679

    KEGG: hsa:7334

    STRING: 9606.ENSP00000316176

    UniGene: Hs.524630