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  4. Recombinant Human Tyrosine-protein kinase receptor UFO (AXL), partial

Recombinant Human Tyrosine-protein kinase receptor UFO (AXL), partial

  • 货号：
    CSB-YP326981HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP326981HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP326981HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP326981HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Adhesion related kinase; AI323647; Ark; Axl; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming gene; AXL transforming sequence/gene; EC 2.7.10.1; JTK11; Oncogene AXL; Tyro7; Tyrosine protein kinase receptor UFO; Tyrosine-protein kinase receptor UFO; UFO; UFO_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.; (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.; (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
  • 基因功能参考文献：
    1. identify the receptor tyrosine kinase Axl as a novel target of ZNF224 transcriptional repression activity. PMID: 30176265
    2. Findings suggest that METTL3 plays very important oncogenic roles in ovarian carcinoma development and/or aggressiveness by stimulating AXL translation and EMT. PMID: 30249526
    3. Co-expression of CDCP1 and AXL is often observed in EGFR-mutation-positive tumors, limiting the efficacy of EGFR TKIs. Co-treatment with EGFR TKI and TPX-0005 warrants testing. PMID: 29433983
    4. This study demonstrates that motility behavior of AXL-expressing tumor cells can be elicited by Gas6-bearing apoptotic bodies generated from tumor treatment with therapeutics that produce killing of a portion of the tumor cells present but not all, hence generating potentially problematic invasive and metastatic behavior of the surviving tumor cells PMID: 28923840
    5. Results show that average methylation in AXL at birth was associated with higher risk for asthma-related phenotypes in childhood, especially wheezing. The effects of average AXL methylation on wheezing symptoms were magnified in girls compared to boys. PMID: 29177020
    6. we identified YAP-driven AXL overexpression as a mechanism of resistance to EGFR TKIs in lung cancer cells. PMID: 29136529
    7. The anti-angiogenic effect of luteolin may be associated with the inhibition of the Gas6/Axl pathway and its downstream phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathways. PMID: 28627676
    8. AXL is influenced by p53 status and could cause the emergence of aggressive clones after exposure to chemotherapy in colon and breast cancer PMID: 28596680
    9. Taken together, these findings suggest that AXL most likely serves as an attachment factor for Zika virus on the cell surface, and that productive infection requires endocytosis and delivery of the virus to acidified intracellular compartments. PMID: 29574335
    10. serum Axl shows high diagnostic accuracy at early stage hepatocellular carcinoma as well as cirrhosis PMID: 28526812
    11. AXL is the only relevant Zika virus entry cofactor expressed on fetal endothelial cells, and that when produced in mammalian cells, only Zika virus, but not West Nile virus or dengue virus, can use AXL, because it more efficiently binds Gas6. PMID: 28167751
    12. Results indicate AXL receptor tyrosine kinase (AXL) as an important mediator of docetaxel resistance in prostate cancer. PMID: 28455956
    13. AXL promotes epithelial cell efferocytosis in a tyrosine kinase-dependent manner.AXL role in AKT-dependent drug resistance. PMID: 28184013
    14. The plasma concentrations of Gas6 and Axl are lowered in rheumatoid arthritis patients. PMID: 24702788
    15. The anti-AXL antibody 20G7-D9 represents a promising therapeutic strategy in triple-negative breast cancer with mesenchymal features by inhibiting AXL-dependent Epithelial-Mesenchymal Transition, tumor growth, and metastasis formation PMID: 27923843
    16. Suppression of AXL by shRNA and inhibitor prolonged survival of chronic myelogenous leukemia (CML) mice and reduced the growth of leukemia stem cells ( LSCs) in mice. Gas6/AXL ligation stabilizes beta-catenin in an AKT-dependent fashion in human CML CD34(+) cells. Our findings improve the understanding of LSC regulation and validate Gas6/AXL as a pair of therapeutic targets to eliminate CML LSCs PMID: 27852702
    17. our data point to a targetable Axl-PI3 kinase-PD-L1 axis that is highly associated with radiation resistance PMID: 28476872
    18. AXL+ and GAS6+ expression is relevant to a poor prognosis in resected lung adenocarcinoma (AD)patients at stage I. AXL/GAS6 might serve as crucial predictive and prognostic biomarkers and targets to identify individuals at high risk of post-operative death. PMID: 28440492
    19. report that Axl regulates FGFR signaling via complex formation with FGFR3 PMID: 26598018
    20. AZD7762 inhibits the proliferative/metastatic activity of breast cancer cells through the suppression of cellular AXL signaling events including anti-apoptosis, migration, invasion, and angiogenesis. PMID: 27829217
    21. BGB324 does not inhibit BCR-ABL1 and consequently inhibits chronic myeloid leukemia (CML)independent of BCR-ABL1 mutational status. Our data show that Axl inhibition has therapeutic potential in BCR-ABL TKI-sensitive as well as -resistant CML and support the need for clinical trials PMID: 27856601
    22. Results show that AXL is upregulated in endometrial cancer tissues and indicate that AXL promotes invasion and migration of endometrial cancer cells. PMID: 27764792
    23. these results suggest that HOTAIR promotes renal cell carcinoma (Rcc) tumorigenesis via miR-217/HIF-1alpha/AXL signaling, which may provide a new target for the diagnosis and therapy of Rcc disease. PMID: 28492542
    24. The expression of MerTK and AxlTK varied according to the deposition of immunoglobulin and complements on glomeruli. Both MerTK and AxlTK expressions were increased on glomeruli and varied according to pathological classifications. PMID: 28127639
    25. High expression level of AXL is associated with lung adenocarcinoma. PMID: 26942465
    26. AXL is a strong adverse prognostic factor for esophageal squamous cell carcinoma PMID: 27172793
    27. Our data demonstrate that AXL is a crucial therapeutic target of carvacrol-induced inhibition of NSCLC cell proliferation and migration. PMID: 29277784
    28. These results show that TYRO3, AXL and GAS6 are expressed at higher levels in LMS and expression of its ligands correlates to a worse PFS in LMS patients. PMID: 29024938
    29. miR-34a reconstitution in DMPM cells significantly inhibited proliferation and tumorigenicity, induced an apoptotic response, and declined invasion ability, mainly through the down-regulation of c-MET and AXL and the interference with the activation of downstream signaling. PMID: 28100259
    30. kinase AXL drives the mesenchymal gene signature and motility of ovarian tumor cells. PMID: 27703029
    31. The AXL inhibitor R428 attenuated RTK and ERK activation and reduced cell motility in Mes cells in culture and reduced tumor growth PMID: 27703030
    32. These results highlight the dual role of Axl during Zika virus infection of glial cells: promoting viral entry and modulating innate immune responses. PMID: 28076778
    33. The expression of AXL was positively associated with GAS6 expression (P < 0.001), and tumor differentiation (P = 0.014) in advanced NSCLC with metastases. AXL expression displayed no association with gender, age, smoking history, pathology, T stage, N stage, CEA, and LDH. PMID: 28551766
    34. Data indicate a role for AXL receptor tyrosine kinase (AXL) in regulating the nuclear translocation of epidermal growth factor receptor (EGFR) and suggest that AXL-mediated SRC family kinases (SFKs) and neuregulin-1 (NRG1) expression promote this process. PMID: 28049763
    35. Combination of high serum levels of soluble AXL and BNP had greater predictive value for heart failure than BNP alone. PMID: 27718443
    36. These results demonstrate that Gas6/Axl axis confers aggressiveness in breast cancer. PMID: 27279912
    37. a lower stability and greater dynamic nature of the Axl kinase domain PMID: 28724631
    38. Silencing AXL or the inhibition of AXL kinase activity significantly inhibits tumor cell migration and invasion in colorectal cancer. PMID: 28727830
    39. we identify PROS1 as a driver of Oral Squamous Cell Carcinoma tumor growth and a modulator of AXL expression PMID: 28118606
    40. Studies indicate that aberrant AXL receptor tyrosine kinase (AXL) signaling and development of an epithelial-to-mesenchymal transition (EMT) phenotype underlie resistance of anaplastic lymphoma kinase (ALK F1174L)-driven neuroblastoma (NB) cells to TAE684 and its derivatives. PMID: 26616860
    41. Studied axl kinase as a negative regulator of an alveolar epithelial cell phenotype; results found that inhibition of Axl kinase could form epithelial adhesion structure and augment surfactant protein production via the MET of human lung multi-potent cells. PMID: 28553934
    42. AXL is efficiently and sequentially cleaved by alpha- and gamma-secretases in various types of cancer cell lines. The AXL intracellular domain cleavage product translocates into the nucleus via a nuclear localization sequence that harbored a basic HRRKK motif. PMID: 28034848
    43. a relationship between AXL and the cellular response to DNA damage whereby abrogation of AXL signaling leads to accumulation of the DNA-damage markers gammaH2AX, 53BP1, and RAD51. PMID: 27893463
    44. Small molecule and antibody inhibitors of AXL and MER have recently been described, and some of these have already entered clinical trials. The optimal design of treatment strategies to maximize the clinical benefit of these AXL and MER targeting agents are discussed in relation to the different cancer types and the types of resistance encountered. PMID: 28251492
    45. our results demonstrate that AR can promote melanoma metastasis via altering the miRNA-539-3p/USP13/MITF/AXL signal and targeting this newly identified signal with AR degradation enhancer ASC-J9 may help us to better suppress the melanoma metastasis. PMID: 27869170
    46. AXL can be an effective therapeutic target in combination with targeted therapy such as PARP inhibitors in several different malignancies. PMID: 27671334
    47. AXL/TAZ/YAP expression is associated with poor prognosis in male breast cancer patients. PMID: 27987320
    48. ablation of AXL has no effect on ZIKA virus (ZIKV) entry or ZIKV-mediated cell death in human induced pluripotent stem cell (iPSC)-derived NPCs or cerebral organoids. PMID: 27912091
    49. the candidate viral entry receptor AXL is highly expressed by human radial glial cells, astrocytes, endothelial cells, and microglia in developing human cortex and by progenitor cells in developing retina. PMID: 27038591
    50. Coexistence of vimentin-positive and Axl-high expression is a poor prognostic factor for primary breast cancer. Vimentin and Axl expression might contribute to the aggressive phenotype in breast cancer. PMID: 27506606

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  • 相关疾病：
    AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thyroid tumorigenesis. Overexpression of AXL and its ligand was also detected in many other cancers such as myeloproliferative disorders, prostatic carcinoma cells, or breast cancer.
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily
  • 组织特异性：
    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 数据库链接：

    HGNC: 905

    OMIM: 109135

    KEGG: hsa:558

    STRING: 9606.ENSP00000301178

    UniGene: Hs.590970