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  4. Recombinant Human Tyrosine-protein kinase CSK (CSK)

Recombinant Human Tyrosine-protein kinase CSK (CSK)

  • 货号：
    CSB-YP006056HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006056HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006056HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006056HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006056HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CSK
  • Uniprot No.：
  • 别名：
    C SRC; C SRC kinase; C src Tyrosine Kinase; C-SRC kinase; c-src tyrosine kinase; Csk A; CSK; CSK_HUMAN; CYTOPLASMIC TYROSINE KINASE; EC 2.7.10.2; MGC112926; MGC117393; MGC154049; P60 Src; Protein tyrosine kinase CYL; Protein-tyrosine kinase CYL; Proto oncogene tyrosine protein kinase; Tyrosine protein kinase CSK; Tyrosine-protein kinase CSK; zgc:154049
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-450
  • 氨基酸序列
    SAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV SCDGKVEHYR IMYHASKLSI DEEVYFENLM QLVEHYTSDA DGLCTRLIKP KVMEGTVAAQ DEFYRSGWAL NMKELKLLQT IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM TQLRHSNLVQ LLGVIVEEKG GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQL EHIKTHELHL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
  • 基因功能参考文献：
    1. Study indicated that MTHFR rs1801133, FGF5 rs16998073 and CSK rs1378942 were associated with increased the risk of obesity in the Chinese children. PMID: 30217759
    2. These results identified a novel role for Csk as a host tyrosine kinase involved in dengue virus replication. PMID: 27457684
    3. Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling PMID: 27815825
    4. Data suggest that myristoylation of Src kinase is essential to facilitate Src-induced and high-fat diet-accelerated prostatic tumor progression; targeting Src kinase myristoylation, which is required for Src kinase association at cellular membrane, blocks dietary fat-accelerated tumorigenesis. PMID: 28939770
    5. Linear mixed-effect regressions showed significant association between rs1378942 in CSK and systolic blood pressure (beta = 0.98+/-0.46, P = 3.4x10-2). The child genotype scores for diastolic and systolic blood pressure were not associated in children. PMID: 29045471
    6. H2O2-induced Csk translocation to the plasma membrane leads to phosphorylation of Src at the tyrosine 530 residue resulting in a reduction of ERK1/2 phosphorylation. PMID: 26234813
    7. Data suggest that as a natural inhibitor of c-Src kinase, naringin could be an effective candidate for the treatment of melanoma. PMID: 26476533
    8. deregulation of the Pragmin-Csk axis may induce aberrant cell migration that contributes to tumor invasion and metastasis PMID: 27116701
    9. Our results do not support association between PTPN22/CSK and Henoch-Schonlein purpura PMID: 26458874
    10. Results identify CSK downregulation as a principal driver of SRC activation and castration resistance. PMID: 26091350
    11. Variant rs1378942 near CSK was nominally associated with systolic blood pressure in a Chinese population. PMID: 25618516
    12. CSK is the kinase responsible for C-terminal phosphorylation of SRC, but not BRK. PMID: 25897081
    13. Data indicate that the potent c-Src inhibitors pyrazolo[3,4-d]pyrimidines showed in vivo activity in neuroblastoma xenograft model. PMID: 25469771
    14. Haemophilus ducreyi LspA1 protein inhibits phagocytosis by activation of host CSK. PMID: 24902122
    15. In Chinese children, no association of CSK rs1378942, MTHFR rs1801133, CYP17A1 rs1004467, STK39 rs3754777 and FGF5 rs16998073 with BP/risk of hypertension. PMID: 23759979
    16. the activity of SIRT2 is regulated by c-Src through post-translational modifications. PMID: 24996174
    17. Data indicate that the accumulation of the cleaved C-terminal small fragment of eukaryotic elongation factor 2 (eEF2) in the nucleus, and C-terminal Src kinase (Csk) could enhance the proteolytic cleavage of eEF2. PMID: 24648518
    18. alpha6beta4 integrin and c-Src activation is important early signaling events to lead mTOR activation and cap-dependent translation of VEGF. PMID: 24180592
    19. Induction of stem cell differentiation alters regulation of FAK and Src activities. PMID: 24015220
    20. JAM-A recruits Csk to the integrin-c-Src complex, where Csk negatively regulates c-Src activation, thereby suppressing the initiation of outside-in signaling. PMID: 24300854
    21. In summary, the free-energy landscape calculations demonstrate that phosphorylation of tyrosine 416 in the A-loop essentially "locks" the kinase into its catalytically competent conformation. PMID: 24103328
    22. An appreciable amount of Y416 was phosphorylated when c-Src was in a closed, repressed conformation under conditions by which c-Src was unable to phosphorylate substrate STAT3. PMID: 23923048
    23. We also unveiled a possible mechanistic link between ASPP2 and Csk/Src signaling pathway, implicating the multiple cellular functions of ASPP2. PMID: 23671128
    24. rs1378942 and rs34933034 variants were not significantly associated with giant cell arteritis. PMID: 23946333
    25. High C-terminal Src kinase expression is associated with colorectal cancer. PMID: 23824743
    26. Ca2+ sparklet activity is promoted by the action of c-Src on tyrosine2122 residue of the Cav1.2c COOH terminus. PMID: 23804206
    27. detailed study of LYP/CSK interaction, here presented, showed that LYP/CSK interaction was inducible upon TCR stimulation, and involved LYP P1 and P2 motifs, and CSK SH3 and SH2 do PMID: 23359562
    28. p140Cap phosphorylation sites and binding to Csk and Abl kinase PMID: 23383002
    29. No significant association was observed for CSK rs1378942 or MTHFR rs17367504 and hypertension in East Asians. PMID: 22959498
    30. This suggests that the Lyp-Csk complex increases susceptibility to lupus at multiple maturation and activation points in B cells. PMID: 23042117
    31. these results indicate that thrombin might activate c-Src to induce JAK2 activation, which in turn, causes STAT3 activation, and finally induces CCN2 expression in human lung fibroblasts. PMID: 23108098
    32. Data provide evidence for replication and overall genome-wide significance for systemic sclerosis (SSc) genetic risk locus: tyrosine-protein kinase CSK with P-value = 5.04 x 10(-12), odds ratio (OR) = 1.20. PMID: 22407130
    33. the association between Furin and its substrate MT1-MMP is regulated by PDGFR stimulation and c-Src. PMID: 22038627
    34. These findings suggest that 25-methoxyhispidol A-mediated inhibitory activity of human breast cancer cell growth might be related with the cell cycle arrest and modulation of signal transduction pathways. PMID: 21782877
    35. c-Src regulates nuclear factor kappa B signaling by targeting NEMO PMID: 21538482
    36. PTPIP51 is phosphorylated by Lyn and c-Src kinases lacking dephosphorylation by PTP1B in acute myeloid leukemia. PMID: 21513978
    37. c-Src signals through protein kinase Calpha, phospholipase Cgamma, and LKB1 to AMPK. AMPK controls internal ribosome entry site (IRES) dependent translation in these cells PMID: 21245141
    38. The ROS-c-Src kinase-mTOR pathway played a central role in the signaling pathway that linked albumin to epithelial mesenchynal transition and endoplasmic reticular stress. PMID: 21367918
    39. Results indicate that Cbp is required for the Csk-mediated inactivation of c-Src and may control the promotion of malignancy in NSCLC tumors that are characterized by c-Src upregulation. PMID: 21156787
    40. Data demonstrate the prognostic role of phospho-Src family kinase in tongue cancer in an inverse correlation between phospho-Src family kinase and patient survival. PMID: 19565264
    41. Cloning of the complete coding sequence of the cDNA of the Csk and Yes tyrosine kinases genes of the human lymphocytes and retina have been carried out. PMID: 19757166
    42. Consistent genetic factors for ATP2B1, CSK, ARSG and CSMD1 were present, which have been shown to be associated with high blood pressure and hypertension in two Korean cohorts. PMID: 19960030
    43. the SH2-domain-mediated activation of Csk is dependent on the binding of the beta3-alphaC loop Ala228 to the hydrophobic pocket formed by the side chains of Tyr116, Tyr133, Leu138, and Leu149 on the surface of the SH2 domain PMID: 20434462
    44. A sublethal dose of myoglobin (100 microg/ml) induced VCAM-1 expression via activation of AP-1 and NF-kappaB, which was mediated through activation of c-Src kinase PMID: 19887846
    45. Csk mis-localization defines a novel mechanism for SFK oncogenic activation in CRC cells. PMID: 20010872
    46. tyrosine kinase Csk dimerizes through Its SH3 domain PMID: 19888460
    47. NMR and site-directed mutagenesis reveal novel mechanism of enzyme regulation PMID: 11724538
    48. Regulation of an ERG K+ current by Src tyrosine kinase. PMID: 11834728
    49. Tyrosine kinase c-Src constitutes a bridge between cystic fibrosis transmembrane regulator channel failure and MUC1 overexpression in cystic fibrosis PMID: 11872746
    50. activation by adaptor protein Shc PMID: 12048194

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  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSK subfamily
  • 组织特异性：
    Expressed in lung and macrophages.
  • 数据库链接：

    HGNC: 2444

    OMIM: 124095

    KEGG: hsa:1445

    STRING: 9606.ENSP00000220003

    UniGene: Hs.77793