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  4. Recombinant Human Tyrosine-protein kinase Blk (BLK)

Recombinant Human Tyrosine-protein kinase Blk (BLK)

  • 货号：
    CSB-YP002714HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002714HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002714HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002714HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002714HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BLK
  • Uniprot No.：
  • 别名：
    B lymphocyte kinase; B lymphoid tyrosine kinase; Blk; BLK nonreceptor tyrosine kinase ; BLK_HUMAN; EC 2.7.10.2; MGC 10442; MGC10442; MODY 11; MODY11; P55 BLK; p55-BLK; Tyrosine kinase B lymphocyte specific ; Tyrosine protein kinase ; Tyrosine-protein kinase BLK
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-505
  • 氨基酸序列
    GLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol.
  • 基因功能参考文献：
    1. our results indicated that the BLK rs13277113 polymorphism was involved in the genetic background of RA in Chinese population and the association of BANK1 rs3733197 polymorphism with RA was dependent on the genotype of BLK rs13277113 polymorphism, highlighting B-cell response implicated in the pathogenesis of RA. PMID: 28925718
    2. present study suggests a novel association between specific TNFSF4 and BLK gene polymorphisms and allergic rhinitis risk. PMID: 28713926
    3. This meta-analysis confirms that polymorphisms in the BLK alleles rs13277113 A/G, rs2736340 T/C, and rs2248932 T/C are associated with susceptibility to SLE in Caucasian and Asian populations. PMID: 27067206
    4. Confirm the association of rs548234/ATG5, rs2736340/BLK and rs10516487/BANK1 with systemic lupus erythematosus in Chinese Han and reinforced our hypothesis of their epistasis effect in regulating B-cell signaling in SLE. PMID: 26420661
    5. ur study provides evidence that human BLK is a true proto-oncogene capable of inducing tumors, and we demonstrate a novel BLK activity-dependent tumor model suitable for studies of BLK-driven lymphomagenesis and screening of novel BLK inhibitors in vivo. PMID: 28670978
    6. rs13277113 GA genotype of BLK is more frequent in Systemic Lupus Erythematosus patients and may have a role in low gene expression and increased flares PMID: 27864698
    7. current meta-analysis suggested that FAM167A-BLK rs2736340 polymorphism is associated with several autoimmune diseases PMID: 27105348
    8. the SNPs in TNFSF4 and FAM167A-BLK may be involved in asthma and allergic rhinitis gene risk in the Han Chinese cohort. PMID: 27088737
    9. The systemic lupus erythematosus variant Ala71Thr of BLK severely decreases protein abundance and binding to BANK1 through impairment of the SH3 domain function. PMID: 26821283
    10. Report a novel BLK gene variant in common variable immunodeficiency-patients that causes suppressed B-cell proliferation and reduced ability of B-cells to elicit antigen-specific CD4(+) T-cell responses. PMID: 25926555
    11. A major mechanism underlying the BLK association with autoimmune disease involves lowered thresholds for basal B cell receptor signaling, enhanced B cell-T cell interactions, and altered patterns of isotype switching. PMID: 26246128
    12. our study reveals a previously unappreciated role of reduced BLK expression on extraperitoneal accumulation of B1a cells in mice, as well as the presence of IgG autoantibodies and B1-like cells in humans. PMID: 25972485
    13. Results support previous findings that vaiants in the RHOB and FAM167A-BLK genes may be associated with susceptibility to systemic sclerosis. PMID: 25470816
    14. These results place Blk upstream of the p190RhoGAP-RhoA pathway in Galpha13-activated cells, overall representing an opposing signaling module during CXCL12-triggered invasion. PMID: 25025568
    15. Report role of BLK genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
    16. The observations suggested that C8orf13-BLK, in combination with STAT4, plays a pivotal role in creating genetic susceptibility to polymyositis/dermatomyositis in Japanese individuals. PMID: 24632671
    17. B-lymphoid tyrosine kinase (Blk) is an oncogene and a potential target for therapy with dasatinib in cutaneous T-cell lymphoma PMID: 24919804
    18. results demonstrated that both lupus-associated functional variants contribute to the autoimmune disease association by modulating transcription of BLK in B cells and thus potentially altering immune responses PMID: 24702955
    19. Our study confirms evidence for epistasis between BLK and BANK1 in systemic lupus erythematosis from a Chinese population for the first time. PMID: 24085759
    20. The BLK region was significantly associated with Kawasaki disease susceptibility in populations of Korean and European descent. PMID: 24023612
    21. BANK1 and BLK have roles in B-cell signaling through phospholipase C gamma 2 PMID: 23555801
    22. SNPs of the FAM167A-BLK region, but not the BANK1 SNPs, were associated with the development of primary Sjogren's syndrome in Han Chinese. PMID: 23899688
    23. This study confirms BANK1 as an RA susceptibility gene and for the first time provides evidence for epistasis between BANK1 and BLK in RA. PMID: 23646104
    24. study demonstrated that the loss-of-function BLK-p.A71T mutation is very unlikely to cause MODY; instead, it may modestly influence type 2 diabetes risk through an interaction with obesity PMID: 23224494
    25. the functional SNP BLK rs2248932 T/C variant allele was associated with rheumatoid arthritis development PMID: 22740142
    26. Blk allele expression differences at the protein level are restricted to early B cells. PMID: 22678060
    27. The genetic variants in the promoter region of BLK may cause dysregulation of BLK expression, which might finally contribute to the initiation and progression of systemic lupus erythematosus. PMID: 22313735
    28. BCR-ABL downregulates the Blk gene (encoding B-lymphoid kinase) through c-Myc in leukemic stem cells in chronic myeloid leukemia PMID: 22797726
    29. Expression of RUNX1 isoforms and its target gene BLK in childhood acute lymphoblastic leukemia. PMID: 22748822
    30. Rare and common regulatory variants in BLK are involved in disease susceptibility in systemic lupus erythematosus. PMID: 22696686
    31. Single nucleotide polymorphism in BLK gene is associated with Kawasaki disease. PMID: 22446961
    32. allelic variation in Blk does not play a major role in determining multifocal motor neuropathy susceptibility. PMID: 22003931
    33. This study shows a genetic interaction between BANK1 and BLK, and demonstrates that these molecules interact physically. PMID: 21978998
    34. Moderate evidence exists for an association between the BLK rs13277113, rs2248932 variants and systemic lupus erythematosus. PMID: 21152986
    35. study evaluated SNP rs2248932 from BLK and further defined its role in systemic lupus erythematosus (SLE) risk; its association with SLE was confirmed in Chinese Han population PMID: 20130895
    36. studies found that IRF5, STAT4 and BLK are associated not only with systemic lupus erythematosus, but also rheumatoid arthritis and systemic sclerosis [review] PMID: 20453440
    37. our results do not support a major implication of the C8orf13-BLK gene region in susceptibility to Giant cell arteritis PMID: 20156505
    38. Results indicate the FAM167A-BLK region may be a shared genetic factor for a number of autoimmune diseases in multiple populations, but the genetic contribution may be grater in Asian populations. PMID: 19740902
    39. genetic polymorphism is associated with systemic sclerosis in North-American and European populations PMID: 19796918
    40. Our findings indicate that the rs13277113A allele is associated not only with SLE but also with SSc and that the FAM167A-BLK region is a common genetic risk factor for both SLE and SSc. PMID: 20131239
    41. transcription of the B cell-specific blk gene is regulated by NERF/ELF-2 and AMl1 PMID: 14970218
    42. identified and confirmed through replication two new genetic loci for SLE: a promoter-region allele associated with reduced expression of BLK and increased expression of C8orf13 and variants in the ITGAM-ITGAX region PMID: 18204098
    43. The association of the C8orf13-BLK region with systemic lupus erythematosus was replicated in a Japanese population. PMID: 19180478
    44. Our data confirmed association of STAT4 (rs7574865, odds ratio (OR) =1.71, P=3.55 x 10(-23)) and BLK (rs13277113, OR=0.77, P=1.34 x 10(-5)) with SLE PMID: 19225526
    45. Blk is constitutively tyrosine phosphorylated in malignant cutaneous T-cell lymphoma cell lines and spontaneously active in kinase assays PMID: 19351960
    46. STAT4 and BLK displayed a strong genetic association with primary antiphospholipid syndrome. PMID: 19644876
    47. Findings point to BLK as a previously unrecognized modulator of beta-cell function, the deficit of which may lead to the development of diabetes. PMID: 19667185
    48. Observational study and genome-wide association study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 19503088

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  • 相关疾病：
    Maturity-onset diabetes of the young 11 (MODY11)
  • 亚细胞定位：
    Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
  • 组织特异性：
    Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
  • 数据库链接：

    HGNC: 1057

    OMIM: 191305

    KEGG: hsa:640

    STRING: 9606.ENSP00000259089

    UniGene: Hs.146591